Alterations of Expression and Distribution of the Ca2+-storing proteins in Endo/Sarcoplasmic Reticulum During Differentiation of Rat Cardiomyocytes

Imanaka‐Yoshida, Kyoko; Amitani, Asaka; Ioshii, Sérgio Ossamu; Koyabu, Sukenari; Yamakado, Tetsu; Yoshida, Toshimichi

doi:10.1006/jmcc.1996.0051

Cited by 33 publications

(18 citation statements)

References 2 publications

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“…However, a similar upregulation does not occur for calreticulin, another ER calcium-binding protein that is highly expressed in developing cardiomyocytes. 21 Such a finding not only indicates that the dedifferentiation process occurs only partially in fibrillating atrial myocytes but reveals GRP94 as the only SR calcium-binding protein that is increased during chronic AF, because no change was reported for calsequestrin. 7 Mechanisms responsible for the GRP94 increase in fibrillating atria remain speculative.…”

Section: Discussionmentioning

confidence: 94%

Increased Myocardial GRP94 Amounts During Sustained Atrial Fibrillation

et al. 2001

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Background-Structural and phenotypic changes of cardiomyocytes characterize atrial fibrillation. We investigated whether changes in the glucose-regulated protein GRP94, which is essential for cell viability, occur in the presence of chronic atrial fibrillation. Methods and Results-Samples of fibrillating atrial myocardium obtained from both goat and human hearts were analyzed for GRP94 expression by an immunologic approach. In goats, atrial fibrillation was induced and maintained for 2, 4, 8, and 16 weeks. After 16 weeks of atrial fibrillation, cardioversion was applied and followed by 8 weeks of sinus rhythm. GRP94 levels doubled in goat atrial myocytes after 4 to 16 weeks of fibrillation with respect to normal atria and returned to control levels in atrial myocardium of cardioverted goats. Immunohistochemical analyses confirm that GRP94 increase occurred within cardiomyocytes. Significantly, increased levels of GRP94 were also observed in samples from human fibrillating atria. In the absence of signs of myocyte irreversible damage, the GRP94 increase in fibrillating atria is comparable to GRP94 levels observed in perinatal goat myocardium. However, calreticulin, another endoplasmic reticulum protein highly expressed in perinatal hearts, does not increase in fibrillating atria, whereas inducible HSP70, a cytoplasm stress protein that is expressed in perinatal goat hearts at levels comparable to those observed in the adult heart, shows a significant increase in chronic fibrillating atria. Conclusions-Our data demonstrate a large, reversible increase in GRP94 in fibrillating atrial myocytes, which may be related to the appearance of a protective phenotype.

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Section: Discussionmentioning

confidence: 94%

Increased Myocardial GRP94 Amounts During Sustained Atrial Fibrillation

et al. 2001

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“…Adult rat cardiomyocytes were isolated from the hearts of 200-250-g male Wistar rats by retrograde perfusion with collagenase as previously described [LoRusso et al, 1992;Imanaka-Yoshida et al, 1993, 1996b. The cells were suspended in Eagle's minimum essential medium supplemented with 10% fetal calf serum and plated onto coverslips (24 ϫ 18 mm) or silicone rubber substrata coated with 20 µg of laminin (Gibco-BRL, Gaithersburg, MD) or 10 cm culture dishes (Falcon, Franklin Lakes, NJ) coated with 100 µg of laminin.…”

Section: Cell Culturesmentioning

confidence: 99%

Vinculin, talin, integrin ?6?1 and laminin can serve as components of attachment complex mediating contraction force transmission from cardiomyocytes to extracellular matrix

Imanaka‐Yoshida

Enomoto‐Iwamoto

Yoshida

et al. 1999

Cell Motil. Cytoskeleton

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“…Structural organization is an important step for cardiac muscle differentiation, and it is independent of muscle-specific protein expression [Imanaka-Yoshida et al, 1996a]. To form and Contract grant sponsors: Japan Heart Foundation; IBM Japan; Japan Foundation of Cardiovascular Research; Ministry of Education, Science and Culture of Japan.…”

Section: Introductionmentioning

confidence: 99%