Alterations in tissue concentration and gene expression of adrenomedullin in spontaneously hypertensive rats

Shimokubo, Toru; Sakata, Junichiro; Kïtamura, Kazuo; Kangawa, Kenji; Matsuo, Hisayuki; Eto, Tanenao

doi:10.1016/0928-4680(95)00038-1

Cited by 9 publications

(5 citation statements)

References 19 publications

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“…Shimokubo et al [23] performed both radioimmunoassay and RNA blot analysis of a range of tissues in spontaneously hypertensive (SHR) and normotensive [Wistar-Kyoto (WKY)] rats. Immunoreactive ADM was higher in the adrenals of SHR and also in cardiac ventricle, kidney and spleen without a difference being seen in other tissues.…”

Section: Plasma and Tissue Adm In Health And Diseasementioning

confidence: 99%

Adrenomedullin

et al. 1996

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Section: Plasma and Tissue Adm In Health And Diseasementioning

confidence: 99%

Adrenomedullin

et al. 1996

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“…[25][26][27] However, in the gastrointestinal tract, the functional role of AM remains obscure. In the present study, the physiological change provoked by 24-h fasting in the rat induced a remarkable increase in the tissue concentration and gene expression level of gastric AM.…”

Section: Discussionmentioning

confidence: 99%

Adrenomedullin in the gastrointestinal tract. Distribution and gene expression in rat and augmented gastric adrenomedullin after fasting

Sakata

Asada

Shimokubo

et al. 1998

Journal of Gastroenterology

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The aim of this study was to investigate the regional distribution, molecular forms, and gene expression of adrenomedullin in the rat gastrointestinal tract and to examine physiological changes in gastric adrenomedullin after 24-h fasting. The tissue concentration was measured by radioimmunoassay. The molecular forms were analyzed by high performance liquid chromatography. mRNA levels were quantified by Northern blotting and cells positive for adrenomedullin immunoreactivity were localized by immunohistochemistry. A high concentration of adrenomedullin was found in stomach, cecum, and colon (450-520 fmol/g wet tissue). Adrenomedullin immunoreactivity was also detected in duodenum, jejunum, and ileum (200-250 fmol/g wet tissue). Transcripts of the adrenomedullin gene were widely expressed throughout the gastrointestinal tract. The major form of adrenomedullin immunoreactivity in stomach and colon corresponded precisely with authentic adrenomedullin peptide. Adrenomedullin immunoreactive cells were present in the gastrointestinal endocrine system. The concentration and mRNA level of gastric adrenomedullin after fasting were significantly increased compared with findings in controls. Adrenomedullin is ubiquitous in the gastrointestinal tract, and may be produced by endocrine cells. The results suggest that adrenomedullin, through its potent vasodilating activity, may play some role, in the stomach including the regulation of the mucosal blood flow.

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“…(3)). In kidneys of SHR and Dahl salt-sensitive rats, neither the peptide nor mRNA expression level of AM was increased in comparison with the respective controls [84,85]. In the meantime, Nishikimi et al reported that both AM content and mRNA expression were augmented in renal medulla of SHR given deoxycorticosterone acetate and salt (DOCA salt SHR), a model of malignant hypertension, concomitantly with elevated plasma AM levels and with increased urinary excretion of AM [87].…”

Section: Plasma Am Level and Production Of Am In Animal Models Of Hypmentioning

confidence: 93%

“…Substantial efforts have so far been made to clarify the pathophysiological role of AM in hypertension by measuring AM concentrations of plasma or tissues and AM mRNA expression in various rat models of hypertension [56,[84][85][86][87]. Shimokubo et al reported that both AM peptide and gene expression levels in the cardiac atrium and ventricle were higher in spontaneously hypertensive rats (SHR) than the control Wistar-Kyoto rats (WKY) [84].…”

Section: Plasma Am Level and Production Of Am In Animal Models Of Hypmentioning

confidence: 99%

“…Shimokubo et al reported that both AM peptide and gene expression levels in the cardiac atrium and ventricle were higher in spontaneously hypertensive rats (SHR) than the control Wistar-Kyoto rats (WKY) [84]. The Dahl saltsensitive rat, a model of volume-dependent hypertension, was examined for AM by at least two groups including ours.…”

Section: Plasma Am Level and Production Of Am In Animal Models Of Hypmentioning

confidence: 99%

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Roles of Adrenomedullin in Hypertension and Hypertensive Organ Damage

Kato¹,

Kïtamura²,

Eto³

2006

CHYR

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Adrenomedullin (AM) is a bioactive peptide having a wide range of biological actions such as vasodilatation, natriuresis, diuresis and inhibition of aldosterone secretion. Various organs and tissues, including the myocardium, vascular wall and kidneys, produce AM, and AM is also present in the bloodstream. Plasma levels of AM were elevated in patients with essential or secondary hypertension as compared with normotensive controls. When infused in a relatively short period of time, AM reduced blood pressure in humans and experimental animals largely through vasodilatation. The blood pressure of transgenic mice overexpressing AM was lower than that of their wild-type littermates; while heterozygotes of AM knockout mice showed higher blood pressure, suggesting a role for endogenous AM in the regulation of blood pressure. Studies with cultured cardiac cells suggest a role for AM in inhibiting hypertrophy or fibrosis of the heart as an autocrine or paracrine factor. Animal experiment studies showed that either prolonged infusion or virally mediated overexpression of AM ameliorated the cardiovascular and renal damage associated with hypertension. Thus, a body of evidence accumulated in this field suggests that AM functions to counteract the elevation in blood pressure and progression of hypertensive organ damage.

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Alterations in tissue concentration and gene expression of adrenomedullin in spontaneously hypertensive rats

Cited by 9 publications

References 19 publications

Adrenomedullin

Adrenomedullin

Adrenomedullin in the gastrointestinal tract. Distribution and gene expression in rat and augmented gastric adrenomedullin after fasting

Roles of Adrenomedullin in Hypertension and Hypertensive Organ Damage

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