Alterations in the rainbow trout (<i>Oncorhynchus mykiss</i>) eggs exposed to ionizing radiation during induced androgenesis

Polonis, Marcin; Jagiełło, Krzysztof; Dobosz, Stefan; Rożyński, Rafał; Kondraciuk, Paulina; Gurgul, Artur; Szmatoła, Tomasz; Ocalewicz, Konrad

doi:10.1111/rda.13413

Cited by 6 publications

(5 citation statements)

References 37 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In C. elegans , as in many species, egg quality and embryogenesis are conditioned by the activity of vitellogenins [ 56 , 61 , 62 , 63 ] and were previously shown to be linked with LD size and their coalescence in the rainbow trout after IR [ 64 ]. In our study we found an increase in LD size and C17Δ decrease with an upregulation of vitellogenins in irradiated worms (SC3).…”

Section: Discussionmentioning

confidence: 99%

Deciphering Differential Life Stage Radioinduced Reproductive Decline in Caenorhabditis elegans through Lipid Analysis

Dufourcq‐Sekatcheff

Cuiné

Li‐Beisson

et al. 2021

IJMS

View full text Add to dashboard Cite

Wildlife is chronically exposed to various sources of ionizing radiations, both environmental or anthropic, due to nuclear energy use, which can induce several defects in organisms. In invertebrates, reproduction, which directly impacts population dynamics, has been found to be the most radiosensitive endpoint. Understanding the underlying molecular pathways inducing this reproduction decrease can help in predicting the effects at larger scales (i.e., population). In this study, we used a life stage dependent approach in order to better understand the molecular determinants of reproduction decrease in the roundworm C. elegans. Worms were chronically exposed to 50 mGy·h−1 external gamma ionizing radiations throughout different developmental periods (namely embryogenesis, gametogenesis, and full development). Then, in addition to reproduction parameters, we performed a wide analysis of lipids (different class and fatty acid via FAMES), which are both important signaling molecules for reproduction and molecular targets of oxidative stress. Our results showed that reproductive defects are life stage dependent, that lipids are differently misregulated according to the considered exposure (e.g., upon embryogenesis and full development) and do not fully explain radiation induced reproductive defects. Finally, our results enable us to propose a conceptual model of lipid signaling after radiation stress in which both the soma and the germline participate.

show abstract

Section: Discussionmentioning

confidence: 99%

Deciphering Differential Life Stage Radioinduced Reproductive Decline in Caenorhabditis elegans through Lipid Analysis

Dufourcq‐Sekatcheff

Cuiné

Li‐Beisson

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…40 min) to the Clinic of Oncology and Radiotherapy, University Clinical Center, Medical University of Gdańsk for irradiation. Irradiation of eggs was performed with a dose of 350 Gy under the same conditions as previously described (Polonis et al 2019). After the treatment, eggs were returned under the same conditions to the hatchery to continue the experiment.…”

Section: Induction Of Androgenesismentioning

confidence: 99%

“…Microsatellite DNA analysis was performed to confirm homozygosity and exclusively paternal inheritance in androgenetic fish. The conditions of DNA extraction and amplification were the same as those described previously (Polonis et al 2019). Three microsatellite markers (loci; OMM: 1107, 1075.…”

Section: Molecular Verification Of Androgenesismentioning

confidence: 99%

“…In rainbow trout, when eggs are not collected within a maximum of 5-7 days after ovulation, biochemical, histological, cellular and molecular changes in eggs may result in their reduced developmental competence (Aegerter & Jalabert 2004). Although hatching and malformation rates (Aegerter & Jalabert 2004;Jagiełło et al 2018;Polonis et al 2019) are considered the most effective indicators of fish egg quality, other biomarkers, including the distribution of lipid droplets (Mansour et al 2007;Ciereszko et al 2009;Polonis et al 2019), pH of the ovarian fluid (Aegerter & Jalabert 2004) and quality and quantity of maternal transcripts (Sullivan et al 2015;Ocalewicz et al 2020) have been proposed for use in assessing the developmental competence of salmonid fish eggs.…”

Section: Introductionmentioning

confidence: 99%

“…The drastic mortality of androgenotes appears to be mainly due to the expression of lethal alleles. Furthermore, side effects of irradiation and exposure to a physical or chemical shock may also affect the survival of embryos developing in such treated eggs (Komen & Thorgaard 2007;Polonis et al 2019).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Inter-clutch egg differences and androgenesis in rainbow trout (Oncorhynchus mykiss, Walbaum 1792)

Polonis

Błaszczyk

Jagiełło

et al. 2021

Oceanological and Hydrobiological Studies

Self Cite

View full text Add to dashboard Cite

Ionizing radiation (IR) is applied to inactivate the nuclear genome in rainbow trout eggs during induced androgenetic development. However, IR-generated reactive oxygen species (ROS) may affect developmental potential of eggs and reduce the effectiveness of androgenesis. To verify this assumption, androgenetic development of rainbow trout was induced in eggs irradiated with 350 Gy of X-rays. Survival rates, pH of the ovarian fluid and activity of antioxidant enzymes, including SOD, CAT and GPx, were examined in non-irradiated and irradiated eggs originating from four females. Survival rates of androgenetic embryos developing in eggs produced by different females varied from 1% to 57% and these inter-clutch differences were significant. Eggs from female F4, which showed the highest developmental competence for androgenesis, also showed increased activities of SOD, CAT and GPx enzymes. The pH value of the ovarian fluid of each female was over 8 before and after irradiation, therefore it seems that radiation did not affect the ovarian fluid pH. Considering the above-mentioned inter-clutch differences, a strong maternal effect on the effectiveness of androgenesis can be assumed. Eggs with increased activity of antioxidant enzymes before irradiation should be expected to show increased developmental competence for androgenesis.

show abstract

Quality of fish eggs and production of androgenetic and gynogenetic doubled haploids (DHs)

Ocalewicz¹

2023

Fish Physiol Biochem

View full text Add to dashboard Cite

Induced development of haploid embryos (H) with only paternal (androgenesis) or maternal (gynogenesis) chromosomes requires irradiation of eggs before fertilization or activation of eggs with irradiated spermatozoa, respectively. To provide doubled haploids (DHs), androgenetic and gynogenetic haploid zygotes need to be subjected to the thermal or high hydrostatic pressure (HHP) shock to suppress the first mitotic cleavage and to double paternal or maternal haploid set of chromosomes. Androgenesis and mitotic gynogenesis (mito-gynogenesis) result in the generation of fully homozygous individuals in a single generation. DHs have been utilized in selective breeding programs, in studies concerning the phenotypic consequences of recessive alleles and to evaluate the impact of sex chromosomes on the early ontogeny. Moreover, the use of DHs for the NGS approach radically improves de novo the assembly of the genomes. However, reduced survival of the doubled haploids limits the wide application of androgenotes and gynogenotes. The high mortality of DHs may be only partly explained by the expression of recessive traits. Observed inter-clutch variation in the survival of DHs developing in eggs originating from different females make it necessary to take a closer look at the quality of the eggs used during induced androgenesis and gynogenesis. Moreover, the developmental competence of eggs that are subjected to irradiation before fertilization in order to deactivate maternal chromosomes when undergoing induced androgenesis and exposed to the physical shock after fertilization that leads to the duplication of the zygotes in both mito-gynogenesis and androgenesis may be also altered as irradiation and sublethal values of temperatures and hydrostatic pressure are considered as harmful for the cell organelles and biomolecules. Here, recently provided results concerning the morphological, biochemical, genomic, and transcriptomic characteristics of fish eggs showing high and low competence for androgenesis and mito-gynogenesis are reviewed.

show abstract

Alterations in the rainbow trout (Oncorhynchus mykiss) eggs exposed to ionizing radiation during induced androgenesis

Cited by 6 publications

References 37 publications

Deciphering Differential Life Stage Radioinduced Reproductive Decline in Caenorhabditis elegans through Lipid Analysis

Deciphering Differential Life Stage Radioinduced Reproductive Decline in Caenorhabditis elegans through Lipid Analysis

Inter-clutch egg differences and androgenesis in rainbow trout (Oncorhynchus mykiss, Walbaum 1792)

Quality of fish eggs and production of androgenetic and gynogenetic doubled haploids (DHs)

Contact Info

Product

Resources

About