Inter-clutch egg differences and androgenesis in rainbow trout (<i>Oncorhynchus mykiss</i>, Walbaum 1792)

Polonis, Marcin; Błaszczyk, Agata; Jagiełło, Krzysztof; Panasiak, Ligia; Dobosz, Stefan; Ocalewicz, Konrad

doi:10.2478/oandhs-2021-0015

Cited by 3 publications

(2 citation statements)

References 35 publications

(50 reference statements)

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“…Androgenetic doubled haploids (DHs) and their normal heterozygous siblings studied in this research were produced and maintained using gamete donors from the spring spawning broodstock of rainbow trout from Rutki strain reared in the Department of Salmonid Research (DSR), Inland Fisheries Institute in Olsztyn (IFI), Rutki, Poland (Polonis et al 2021 ). Shortly, batches of eggs were divided in to two groups; one designated for irradiation and induction of androgenesis and the second batch to serve as control (normal fertilization).…”

Section: Methodsmentioning

confidence: 99%

Telomere length variation does not correspond with the growth disturbances in the rainbow trout (Oncorhynchus mykiss)

et al. 2021

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Somatic growth is considered to affect pace of the telomere attrition in vertebrates. As normally developed and dwarf fish differ in the body size we have decided to compare telomere length in the rainbow trout (Oncorhynchus mykiss) with normal growth and with growth reduced due to the dwarf condition. Examined 1-year-old fish with normal and dwarf appearance were siblings originated from androgenetic fully homozygous doubled haploid (DH) line of rainbow trout. Particular dwarf individuals had body deformities such as humpback, kyphosis, and lordosis. Somatic cells of examined rainbow trout had an average telomere length between 17 and 20 kb, comparable in females and males. Dwarf rainbow trout exhibited significantly lower body length and weight than their normally developed siblings even though no differences in the telomere length were found between these fishes. Statistical analysis did not exhibit any correlation between body size and the telomere length. Equal length of telomeres observed in the studied normal and dwarf rainbow trout suggests morphological and physiological differences in fish with different growth rates do not affect dynamics of telomeric DNA. Or any variation in the telomere length might have been levelled by telomerase that in rainbow trout is active in all tissues irrespective of the individual developmental stage.

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Section: Methodsmentioning

confidence: 99%

Telomere length variation does not correspond with the growth disturbances in the rainbow trout (Oncorhynchus mykiss)

et al. 2021

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show abstract

“…Androgenetic Doubled Haploids (DHs) and their normal heterozygous siblings studied in this research were produced and maintained using gamete donors from the spring spawning broodstock of rainbow trout from Rutki strain reared in the Department of Salmonid Research (DSR), Inland Fisheries Institute in Olsztyn (IFI), Rutki, Poland (Polonis et al 2021). Shortly, batches of eggs were divided in to two groups; one designated for irradiation and induction of androgenesis and the second batch to serve as control (normal fertilization).…”

Section: Fish Origin and Breeding Conditionsmentioning

confidence: 99%

Telomere length variation does not correspond with the growth disturbances in the rainbow trout (Oncorhynchus mykiss)

Ocalewicz

Panasiak

Szubert

et al. 2021

Preprint

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Somatic growth is considered to affect pace of the telomere attrition in vertebrates. As normally developed and dwarf fish differ in the body size we have decided to compare telomere length in the rainbow trout (Oncorhynchus mykiss) with normal growth and with growth reduced due to the dwarf condition. Examined on-year old fish with normal and dwarf appearance were siblings originated from androgenetic fully homozygous doubled haploid (DH) line of rainbow trout. Particular dwarf individuals had body deformities such as humpback, kyphosis and lordosis. Somatic cells of examined rainbow trout had an average telomere length between 17 and 20 kb, comparable in females and males. Dwarf rainbow trout exhibited significantly lower body length and weight than their normally developed siblings even though, no differences in the telomere length were found between these fishes. Statistical analysis did not exhibit any correlation between body size and the telomere length. Equal length of telomeres observed in the studied normal and dwarf rainbow trout suggests morphological and physiological differences in fish with different growth rates do not affect dynamics of telomeric DNA. Or any variation in the telomere length might have been levelled by telomerase that in rainbow trout is active in all tissues irrespective of the individual developmental stage.

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Quality of fish eggs and production of androgenetic and gynogenetic doubled haploids (DHs)

Ocalewicz¹

2023

Fish Physiol Biochem

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Induced development of haploid embryos (H) with only paternal (androgenesis) or maternal (gynogenesis) chromosomes requires irradiation of eggs before fertilization or activation of eggs with irradiated spermatozoa, respectively. To provide doubled haploids (DHs), androgenetic and gynogenetic haploid zygotes need to be subjected to the thermal or high hydrostatic pressure (HHP) shock to suppress the first mitotic cleavage and to double paternal or maternal haploid set of chromosomes. Androgenesis and mitotic gynogenesis (mito-gynogenesis) result in the generation of fully homozygous individuals in a single generation. DHs have been utilized in selective breeding programs, in studies concerning the phenotypic consequences of recessive alleles and to evaluate the impact of sex chromosomes on the early ontogeny. Moreover, the use of DHs for the NGS approach radically improves de novo the assembly of the genomes. However, reduced survival of the doubled haploids limits the wide application of androgenotes and gynogenotes. The high mortality of DHs may be only partly explained by the expression of recessive traits. Observed inter-clutch variation in the survival of DHs developing in eggs originating from different females make it necessary to take a closer look at the quality of the eggs used during induced androgenesis and gynogenesis. Moreover, the developmental competence of eggs that are subjected to irradiation before fertilization in order to deactivate maternal chromosomes when undergoing induced androgenesis and exposed to the physical shock after fertilization that leads to the duplication of the zygotes in both mito-gynogenesis and androgenesis may be also altered as irradiation and sublethal values of temperatures and hydrostatic pressure are considered as harmful for the cell organelles and biomolecules. Here, recently provided results concerning the morphological, biochemical, genomic, and transcriptomic characteristics of fish eggs showing high and low competence for androgenesis and mito-gynogenesis are reviewed.

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Inter-clutch egg differences and androgenesis in rainbow trout (Oncorhynchus mykiss, Walbaum 1792)

Cited by 3 publications

References 35 publications

Telomere length variation does not correspond with the growth disturbances in the rainbow trout (Oncorhynchus mykiss)

Telomere length variation does not correspond with the growth disturbances in the rainbow trout (Oncorhynchus mykiss)

Telomere length variation does not correspond with the growth disturbances in the rainbow trout (Oncorhynchus mykiss)

Quality of fish eggs and production of androgenetic and gynogenetic doubled haploids (DHs)

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