Alterations in the electrical properties of T and B lymphocyte membranes induced by mitogenic stimulation. Activation monitored by electro-rotation of single cells

Hu, Xun; Arnold, W.M.; Zimmermann, U.

doi:10.1016/0005-2736(90)90033-k

Cited by 87 publications

(62 citation statements)

References 24 publications

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“…Specific capacitance of the plasma membrane reflects membrane morphological features, including microvilli, membrane folds, and blebbing (12,16,17). Thus, the large differences between MDA231 cells, lymphocytes, and erythrocytes found here almost certainly arise from membrane morphological differences.…”

Section: Discussionmentioning

confidence: 93%

“…The frequency dependencies of the magnitude and direction of these forces are functions of the intrinsic electrical properties of the particle that depend on the particle constitution and structural organization (13)(14)(15). For living cells, these characteristics are defined by composition, morphology, and phenotype (16,17). Thus, DEP and ROT have been used to study bacteria (18), yeasts (11,19), plant cells (10), and mammalian cells (20,21) and to investigate cellular alterations accompanying physiological changes such as mitotic stimulation (16) and induced differentiation (7,17,21).…”

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confidence: 99%

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Separation of human breast cancer cells from blood by differential dielectric affinity.

Becker

Wang

Huang

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

644

508

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Electrorotation measurements were used to demonstrate that the dielectric properties of the metastatic human breast cancer cell line MDA231 were significantly different from those of erythrocytes and T lymphocytes. These dielectric differences were exploited to separate the cancer cells from normal blood cells by appropriately balancing the hydrodynamic and dielectrophoretic forces acting on the cells within a dielectric affinity column containing a microelectrode array. The operational criteria for successful particle separation in such a column are analyzed and our findings indicate that the dielectric affinity technique may prove useful in a wide variety of cell separation and characterization applications.Cell separation has numerous applications in medicine, biotechnology, and research and in environmental settings. For example, the use of autologous bone marrow transplants in the remediation of advanced cancers requires the removal of cancer cells from the patient's marrow (1), the study of signaling between blood cells requires purified cell subpopulations (2), and the purification of contaminated water supplies necessitates elimination of parasites such as Giardia and Cryptosporidium (3,4). Current sorting technologies usually exploit differences in cell density, immunologic targets, or receptor-ligand interactions. These techniques are often inadequate, producing insufficiently pure cell populations, being too slow, or being too limited in the spectrum of target cells. Identification of novel properties by which different cell types may be discerned and of new ways for their selective manipulation are clearly fundamental components for improving sorting methodologies.A particle suspended in a medium of different dielectric characteristics becomes electrically polarized when subjected to an alternating electrical field. Interaction between this induced polarization and the field gives rise to various electrokinetic effects. For example, a spatially inhomogeneous field will exert a lateral dielectrophoretic (DEP) force on the particle, directing it toward the minimum of dielectric potential (5-9), while a rotating field will induce particle electrorotation (ROT; refs. 10-12). The frequency dependencies of the magnitude and direction of these forces are functions of the intrinsic electrical properties of the particle that depend on the particle constitution and structural organization (13-15). For living cells, these characteristics are defined by composition, morphology, and phenotype (16, 17). Thus, DEP and ROT have been used to study bacteria (18), yeasts (11,19), plant cells (10), and mammalian cells (20,21) and to investigate cellular alterations accompanying physiological changes such as mitotic stimulation (16) and induced differentiation (7,17,21 MATERIALS AND METHODS Cells. Peripheral blood was collected by venipuncture into 90 parts Ca2+/Mg2+-free phosphate-buffered saline containing 5 mM hemisodium EDTA to prevent clotting. T lymphocytes were obtained from (human immunodeficiency virusa...

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Section: Discussionmentioning

confidence: 93%

mentioning

confidence: 99%

Separation of human breast cancer cells from blood by differential dielectric affinity.

Becker

Wang

Huang

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

644

508

View full text Add to dashboard Cite

show abstract

“…30,43 DEP is frequently used as a label-free method in particle separation and isolation since DEP events are specifically dictated by inherent particle and media properties. 38,[44][45][46] For biological cells, the DEP-induced behavior is governed by surface morphology, extracellular proteins, and intracellular events. This DEP property has been leveraged to separate and enrich cancer cells from other types of cells.…”

Section: Potential Application In Cancer Research and Diagnosticsmentioning

confidence: 99%

“…Selective isolation of a specific particle can be achieved by applying an additional non-electrical force into the system. In the low frequency region, the conductivity terms compose what is referred to as the Claussius-Mossotti factor, K(x) 38 …”

Section: Introductionmentioning

confidence: 99%

Separation of tumor cells with dielectrophoresis-based microfluidic chip

et al. 2013

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The present work demonstrates the use of a dielectrophoretic lab-on-a-chip device in effectively separating different cancer cells of epithelial origin for application in circulating tumor cell (CTC) identification. This study uses dielectrophoresis (DEP) to distinguish and separate MCF-7 human breast cancer cells from HCT-116 colorectal cancer cells. The DEP responses for each cell type were measured against AC electrical frequency changes in solutions of varying conductivities. Increasing the conductivity of the suspension directly correlated with an increasing frequency value for the first cross-over (no DEP force) point in the DEP spectra. Differences in the cross-over frequency for each cell type were leveraged to determine a frequency at which the two types of cell could be separated through DEP forces. Under a particular medium conductivity, different types of cells could have different DEP behaviors in a very narrow AC frequency band, demonstrating a high specificity of DEP. Using a microfluidic DEP sorter with optically transparent electrodes, MCF-7 and HCT-116 cells were successfully separated from each other under a 3.2 MHz frequency in a 0.1X PBS solution. Further experiments were conducted to characterize the separation efficiency (enrichment factor) by changing experimental parameters (AC frequency, voltage, and flow rate). This work has shown the high specificity of the described DEP cell sorter for distinguishing cells with similar characteristics for potential diagnostic applications through CTC enrichment. V C 2013 American Institute of Physics. [http://dx

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“…This work has been continued recently by some of the same authors [48]. ED has also been reported, on several occasions, by U. Zimmermann and co-workers, among their very extensive electro-manipulation literature, which includes such topics as: DEP and ER [89], the effects of ED on EP [38], and the effects of ultra-short pulses [90]. They have also reported ED of non-RBC mammalian (murine Sp2 myeloma) cells [46].…”

Section: Electro-deformation (Ed) Of Cellsmentioning

confidence: 78%