Alterations in size, shape and osmotic behaviour of red cells after splenectomy: a study of their age dependence

Haan, Lydia de; Werre, J. M.; Ruben, A. M. Th.; Huls, H. A.; Gier, J. De; Staal, Gerard E.J.

doi:10.1111/j.1365-2141.1988.tb07605.x

Cited by 13 publications

(12 citation statements)

References 21 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In older erythrocytes absolute increases of HbA1c and HbA1e2 are measured. Also, an increase in total hemoglobin content and an increase in surface area relative to those of control old cells could be demonstrated [33,34]. Taken together, these observations point towards the retention of vesicles especially in erythrocytes containing older hemoglobin.…”

Section: The Role Of Vesiculation During Erythrocyte Agingsupporting

confidence: 62%

Erythrocyte Aging: A More than Superficial Resemblance to Apoptosis?

Bosman

Willekens

Werre

2005

Cell Physiol Biochem

211

151

View full text Add to dashboard Cite

In physiological circumstances, erythrocyte aging leads to binding of autologous IgG followed by recognition and removal through phagocytosis, mainly by Kupffer cells in the liver. This process is triggered by the appearance of a senescent erythrocyte-specific antigen. The functional and structural characteristics of senescent erythrocytes strongly suggest that this antigen originates on band 3, probably by calcium-induced proteolysis. Generation of vesicles enriched in denatured hemoglobin is an integral part of the erythrocyte aging process. These versicles are also removed by Kupffer cells, with a major role for exposure of phosphatidylserine. Moreover, senescent erythrocyte-specific antigens are present on vesicles. Thus, vesicles and senescent erythrocytes may be recognized and removed through the same signals. These and other, recent data support the theory that erythrocyte aging is a form of apoptosis that is concentrated in the cell membrane, and provide the context for future studies on initation and regulation of the erythrocyte aging process. Insight into the normal aging mechanism is essential for understanding the fate of erythrocytes in pathological circumstances and the survival of donor erythrocytes after transfusion.

show abstract

Section: The Role Of Vesiculation During Erythrocyte Agingsupporting

confidence: 62%

Erythrocyte Aging: A More than Superficial Resemblance to Apoptosis?

Bosman

Willekens

Werre

2005

Cell Physiol Biochem

211

151

View full text Add to dashboard Cite

show abstract

“…24 25 Another possible use of this flow cytometry technique is the assessment of the evolution of the RBC shape after splenectomy. 26 Using flow cytometry, we observed that the more spherical shape of the RBCs in patients with diabetes was not correlated with blood sugar or the severity of the disease as assessed by the HbA 1C . These RBC alterations could increase the risk of peripheral ischaemia in these patients, especially if blood viscosity is increased.…”

Section: Discussionmentioning

confidence: 84%

Assessment of erythrocyte shape by flow cytometry techniques

Piagnerelli¹,

Boudjeltia²,

Brohee³

et al. 2006

J Clin Pathol

View full text Add to dashboard Cite

show abstract

“…Splenectomy prolongs survival of HS red cells and slows down the decrease in deformability compared to patients that have not undergone splenectomy [20]. The effect of splenectomy is less in HS caused by band 3 deficiency [21].…”

Section: Discussionmentioning

confidence: 99%

Partial pyruvate kinase deficiency aggravates the phenotypic expression of band 3 deficiency in a family with hereditary spherocytosis

et al. 2014

View full text Add to dashboard Cite

In a family with mild dominant spherocytosis, affected members showed partial band 3 deficiency. The index patient showed more severe clinical symptoms than his relatives, and his red blood cells displayed concomitant low pyruvate kinase activity. We investigated the contribution of partial PK deficiency to the phenotypic expression of mutant band 3 in this family. Pyruvate kinase deficiency and band 3 deficiency were characterized by DNA analysis. Results of red cell osmotic fragility testing, the results of cell deformability obtained by the Automated Rheoscope and Cell Analyzer and the results obtained by Osmotic Gradient Ektacytometry, which is a combination of these tests, were related to the red cell ATP content. Spherocytosis in this family was due to a novel heterozygous mutation in SLC4A1, the gene for band 3. Reduced PK activity of the index patient was attributed to a novel mutation in PKLR inherited from his mother, who was without clinical symptoms. Partial PK deficiency was associated with decreased red cell ATP content and markedly increased osmotic fragility. This suggests an aggravating effect of low ATP levels on the phenotypic expression of band 3 deficiency.Am. J. Hematol. 90:E35-E39,

show abstract

Alterations in size, shape and osmotic behaviour of red cells after splenectomy: a study of their age dependence

Cited by 13 publications

References 21 publications

Erythrocyte Aging: A More than Superficial Resemblance to Apoptosis?

Erythrocyte Aging: A More than Superficial Resemblance to Apoptosis?

Assessment of erythrocyte shape by flow cytometry techniques

Partial pyruvate kinase deficiency aggravates the phenotypic expression of band 3 deficiency in a family with hereditary spherocytosis

Contact Info

Product

Resources

About