Alterations in Serum Immunoglobulin G Subclasses in Patients With-Ulcerative Colitis and Crohn's Disease

MacDermott, Richard P.; Nash, Geoffrey S.; Auer, I. O.; Shlien, R.; Lewis, B.S.; Madassery, J.; Nahm, Moon H.

doi:10.1016/s0016-5085(89)80074-5

Cited by 16 publications

(24 citation statements)

References 20 publications

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“…Furthermore, serum from CTT with spontaneous colitis had circulating antibodies against the 7E|2H|2-reactive protein, whereas normal CTT did not [24]. A disease-specific amplification of IgGl response in UC ha;i been shown both by immunocylochemical analysis in ihe coioiiic mucosa [25] and from circulating antibodies [26]. Using triple-colour immunofluorescence technique.…”

Section: Discussionmentioning

confidence: 97%

Expression of a unique protein on colon cancer cells that reacts with a novel monoclonal antibody and ulcerative colitis serum

Hassan

Kanisawa

Meyers

et al. 1995

Clinical and Experimental Immunology

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SUMMARYWe earlier developed a MoAb, 7E|2H[2 (IgM isotype), against a protein present in normal colonic epithelial cells. To examine if 7E,3Hi2-reactive protein is expressed in colon cancer cells and is recognized by ulcerative colitis (UC}-associated autoantibody. we investigated several colon cancer cell lines. 7E12H12 reactivity against the cells was examined by indirect immunofluorescence assay and whole cell HLISA against six colon cancer cell lines HT-29, LoVo, COLO 205, DLD-1. LS 180 and SW 1116, A competitive ELISA was developed using 7E|.H|. MoAb and patients' serum to examine the cross-reactive antibodies in the serum. Among the six colon cancer cell lines only LS 130, DLD-! and SW 1116 reacted with 7E12H1. MoAb. while others did not. The mean (is.e.m.) inhibition of the binding or7Ei^H]2 MoAb to LS 180 cells by UC serum (n=5l) was 42±2iyo, whereas in normal subjects («=I7) it was I4±2-6%, in Crohn's disease (/T=19) it was 15-3±2-5%. in infectious diarrhoea (n^IO) it was 11%±3%. and in systemic lupus erythematosus («=10) it was 2% ±0-6%. The inhibition by the UC group was significantly (/'<0 OOI-

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Section: Discussionmentioning

confidence: 97%

Expression of a unique protein on colon cancer cells that reacts with a novel monoclonal antibody and ulcerative colitis serum

Hassan

Kanisawa

Meyers

et al. 1995

Clinical and Experimental Immunology

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“…In this regard, increased leveis of IgG (IgGl and lgG3 in UC. IgG I and IgG2 in CD) have been described in IBD [55]. Enhanced production of IL-6 may contribute lo increased IgG synihesis and secretion in the lamina propria, and coupled with subsequent complement activation, could result in mucosal injury and inflammation.…”

Section: Discussionmentioning

confidence: 99%

Enhand secretion of tumour necrosis factor-alpha, IL-6, and IL-1β by isolated lamina ropria monouclear cells from patients with ulcretive cilitis and Crohn's disease

Reinecker

Steffen

Witthoeft

et al. 1993

Clinical and Experimental Immunology

804

343

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The perpetuation of inflammation in ulcerative colitis and Crohn's disease may be regulated in part by an increased secretion of proinflammatory cytokines due to either an appropriate response to initial stimulating agents, and/or due to an impaired down-regulation of cytokine secretion. The aim of this study was to determine the secretion patterns of the proinflammatory cytokines tumour necrosis factor-alpha (TNF-alpha), IL-6 and IL-1 beta, from isolated lamina propria mononuclear cells (LPMNC) isolated from colonic biopsies from patients with untreated ulcerative colitis or Crohn's disease. LPMNC isolated from involved inflammatory bowel disease (IBD) mucosa spontaneously produced increased amounts of TNF-alpha, and IL-6, and IL-1 beta. The TNF-alpha secretion from IBD LPMNC could be further enhanced by pokeweed mitogen stimulation. The secretion patterns of TNF-alpha and IL-1 beta by LPMNC from patients with either ulcerative colitis or Crohn's disease demonstrated a close correlation with the degree of tissue involvement and mucosal inflammation. LPMNC from non-involved ulcerative colitis mucosa secreted markedly increased levels of IL-6 compared with non-involved Crohn's disease mucosa or control mucosa. The heightened IL-6 secretion from LPMNC from non-involved ulcerative colitis mucosa without visible or microscopic signs of inflammation indicates that the pathophysiologic mechanisms involved in the initiation of inflammation may differ between ulcerative colitis and Crohn's disease. The determination of proinflammatory cytokine secretion by isolated LPMNC from colonoscopic biopsies may be a sensitive method for monitoring the severity of mucosal inflammation in IBD patients.

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“…This finding is partly in accordance with the results of Scott et al [17], who examined the spontaneous secretion of IgG subclasses by intestinal mononuclear cells from patients with UC, and found a mark edly increased production of both IgGl and IgG3. Recently they reported similar altera tions in the serum IgG subclasses in patients with UC [22], The elevation of the number of IgG3 cells may be an overcompensation of increased losses of this subtype of immuno globulin. IgG3 has a long hinge region that is easily accessible to proteases [9]; protease activity is increased in the mucosa of inflam matory bowel disease [2].…”

Section: Discussionmentioning

confidence: 77%

IgG3 and IgG4 Cells Are Increased in Active Ulcerative Colitis

Arató

Savilahti

1990

Digestion

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Cells containing various subclasses of IgG and IgA were counted in the rectal and colonic mucosae from 14 pediatric patients with ulcerative colitis (UC) and in rectal biopsy specimens from 10 control subjects using monoclonal antibodies and the peroxidase-staining method. In both the patients and controls, the IgG1 cells predominated. The numbers of IgG1, IgG2, IgG3 and IgG4 cells were highest in the colonic specimens of patients with UC. The numbers of IgG3- and IgG4-containing cells were increased in the rectal mucosa of untreated UC patients compared to controls. In the rectal mucosa of patients with UC, the percentage of IgG2 cells was decreased compared to the controls (20 vs. 28%; p < 0.05). In the great majority (37 out of 40) of specimens the number of IgA1 cells was higher than that of IgA2. The median number of IgA1 cells in the rectal specimens of untreated patients was slightly higher than that in the rectal specimens of the controls, for IgA2 the numbers were similar. Accentuation of colorectal IgG3 and IgG4 responses may be characteristic early changes in UC.

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Alterations in Serum Immunoglobulin G Subclasses in Patients With-Ulcerative Colitis and Crohn's Disease

Cited by 16 publications

References 20 publications

Expression of a unique protein on colon cancer cells that reacts with a novel monoclonal antibody and ulcerative colitis serum

Expression of a unique protein on colon cancer cells that reacts with a novel monoclonal antibody and ulcerative colitis serum

Enhand secretion of tumour necrosis factor-alpha, IL-6, and IL-1β by isolated lamina ropria monouclear cells from patients with ulcretive cilitis and Crohn's disease

IgG3 and IgG4 Cells Are Increased in Active Ulcerative Colitis

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