Alterations in macromolecular synthesis and cellular growth in mouse embryo fibroblasts infected with friend leukemia virus

Gabelman, Norman; Scher, William; Friend, Charlotte

doi:10.1002/ijc.2910130310

Cited by 7 publications

(1 citation statement)

References 15 publications

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“…For example, the survival times of the hematopoietic stem cells of FLV-P-infected murine bone marrow cultures were extended and granulopoiesis was stimulated (6,29). In addition, FLV affected the proliferative capacity of mouse embryo fibroblasts (30), and continuous lines have been isolated from such infected cultures (31). In vitro infection with FLV-P or FLV-A (19) led to the development of immortalized cell lines.…”

Section: Methodsmentioning

confidence: 99%

In vitro transformation of mouse bone marrow cells by the polycythemic strain of Friend leukemia virus.

Revoltella¹,

Bertolini²,

Friend³

1979

Proc. Natl. Acad. Sci. U.S.A.

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Strains of Friend leukemia virus (FLV) that are associated with polycythemia contain the defective spleen focus-forming virus (SFFV). To determine whether the transforming ability of FLV was affected by the presence of this second agent, DBA/2J mouse bone marrow cells were infected in vitro. Criteria for transformation were the establishment of permanent lines, growth on semisolid agarose, and the production of tumors at the site of inoculation in syngeneic hosts. (FLV) causes erythroleukemia in susceptible strains of adult mice after a short latent period (1, 2). This model resembles Di Guglielmo disease in humans in that it is characterized by a rapid proliferation of primitive hematopoietic cells and is associated with an erythropoietic response. Although FLV has been shown to influence the activity of other types of blood cells (3-6), the primary target for FLV in vivo is an erythroid precursor (7-9). While the prototype virus (FLV-A) induces erythroleukemia accompanied by anemia (1, 2), some strains, designated FLV-P, induce the same type of erythroleukemia but associated with polycythemia (10). Antiserum to either strain neutralizes the infectivity of the other, although FLV-P is thought to contain a complex of viruses, among which is the defective spleen focus-forming virus (SFFV) (11). The polycythemia is attributed to its activity. Removal of SFFV from FLV-P preparations eliminates the polycythemia-inducing factor without altering the leukemogenic activity (12, 13). Thus, the role of SFFV in the pathogenesis of FLV-induced erythroleukemia in vio is not clear. SFFV is expressed only in the presence of a "helper," which may be supplied by a variety of viruses and nonviral agents (14-18).We have compared the ability of the two FLV strains to transform hematopoietic cells in vitro in an effort to clarify the role that SFFV may play in the pathogenesis of the leukemia in vivo. The preceding report (19) described the transformation of mouse fetal liver cells infected in vitro with FLV-A, which lacks SFFV activity. The results of parallel studies on the transformation of mouse bone marrow cells infected with FLV-P in vitro is the subject of this communication.The criteria for transformation were altered morphology, establishment of cells in continuous culture, growth on soft agar, and tumorigenicity in syngeneic hosts. Two permanent lines of malignant hematopoietic cells originated from cultures of DBA/2 mouse bone marrow cells infected in vitro with FLV-P. Each grows in suspension, can be cloned on soft agar, and produces tumors in syngeneic mice. The erythroid nature of the transformed cells was verified by stimulation of differentiation after treatment with dimethyl sulfoxide (Me2SO) (9) and hexamethylene bisacetamide (HMBA) (20). MATERIALS AND METHODSVirus. Stock FLV-P virus was prepared from filtrates of leukemic spleens of polycythemic DBA/2J male mice (Jackson Laboratory), as described (1), and stored at -180°C. The LD5o (mean lethal dose) of the virus titered for leukemogenicity in 6-to 8-wee...

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Section: Methodsmentioning

confidence: 99%

In vitro transformation of mouse bone marrow cells by the polycythemic strain of Friend leukemia virus.

Revoltella¹,

Bertolini²,

Friend³

1979

Proc. Natl. Acad. Sci. U.S.A.

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Appearance of C‐type virus‐like particles after co‐cultivation of a human tumor‐cell line with rat (XC) cells

Gabelman

Waxman

Smith

et al. 1975

Intl Journal of Cancer

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A serially progagated cell line (L104) was established by co-cultivation of alung adenocarcinoma (L-1) from a patient with concurrent chronic lymphocytic leukemia and XC, a non-producer rat line, known to carry the Rous sarcoma virus (RSV) genome. Karyotype of the L104 cultures revealed predominantly rat-like patterns; however, about 5% of the cells reacted with HLA antibodies and demonstrated human isozyme patterns. Electron microscopy of L104 cells revealed the presence of C-type particles budding from the cell membranes and in cytoplasmic vacuoles. Virus was not detected in any of the other normal lung, lung tumor or XC cells examined after co-cultivation with XC cells. The particles isolated from tissue culture fluids had the biochemical and biophysical characteristics common to other known mammalian C-type particles and were serologically related to the woolly monkey virus (WMV)/gibbon ape leukemia virus (GaLV) complex. Cross-hybridization between viral 3H-DNA transcripts and cellular RNAs from virus-infected cells clearly show the presence of sequences in the L104 cellular RNA related to both the GaLV/WMV group of viruses and rat viruses. Hydroxyapatite chromatography reveals however that the primate-related sequences in the viral RNA are indistinguishable from WMV in thermal elution profile. The host range of L104 virus appears to vary greatly from WMV in being xenotropic and, in the cell lines thus far tested appears, to infect only rat cells. The virus gave positive KC but negative XC assays. Inoculation of whole cells or cell-free supernatants into weaning hamster did not result in either solid tumors or leukemia. Co-cultivation of appropriate cell lines may represent an approach to the detection of latent viruses in human neoplasia.

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Xenotropic Type C Viruses

Levy

1978

Modern Aspects of Electrochemistry

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Alterations in macromolecular synthesis and cellular growth in mouse embryo fibroblasts infected with friend leukemia virus

Cited by 7 publications

References 15 publications

In vitro transformation of mouse bone marrow cells by the polycythemic strain of Friend leukemia virus.

In vitro transformation of mouse bone marrow cells by the polycythemic strain of Friend leukemia virus.

Appearance of C‐type virus‐like particles after co‐cultivation of a human tumor‐cell line with rat (XC) cells

Xenotropic Type C Viruses

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