Alterations in follicular fluid estradiol, progesterone and insulin concentrations during ovarian acyclicity in water buffalo (Bubalus bubalis)

Khan, Firdous Ahmad; Das, G. C.; Pande, Megha; Sarkar, Mihir; Mahapatra, R. K.; Shankar, Uma

doi:10.1016/j.anireprosci.2011.12.020

Cited by 18 publications

(8 citation statements)

References 39 publications

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“…Furthermore, the decreased levels of Cyp1b1, a rate-limiting enzyme in estrogen metabolism (31), may also be responsible for increasing the level of E 2 production. Previous studies demonstrated that E 2 and P 4 are indispensable for maintaining normal physiological function in the ovary (19). Thus we infer that ATF6 may be involved in the process of folliculogenesis, follicular growth, and ovulation by regulating the P 4 and E 2 production in granulosa cells.…”

Section: Discussionmentioning

confidence: 63%

ATF6 knockdown decreases apoptosis, arrests the S phase of the cell cycle, and increases steroid hormone production in mouse granulosa cells

Xiong

Chen

Lin

et al. 2017

American Journal of Physiology-Cell Physiology

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Activating transcription factor 6 (ATF6), a sensor protein located in the endoplasmic reticulum (ER) membrane, is an important factor in the ER stress signaling pathway. ER stress is known to be involved in folliculogenesis, follicular growth, and ovulation; however, the physiological function of ATF6 in mouse granulosa cells remains largely unknown. The aim of this study was to assess the role of ATF6 in mouse granulosa cells with respect to apoptosis, the cell cycle, and steroid hormone production, as well as several key genes related to follicular development, via RNA interference, immunohistochemical staining, real-time quantitative PCR, Western blotting, flow cytometry, terminal deoxynucleotidyltransferase-mediated deoxy-UTP nick end labeling (TUNEL) assay, and ELISA. Immunohistochemical staining revealed that ATF6 was extensively distributed in the granulosa cells of various ovarian follicles and oocytes in adult female mice. FSH or LH treatment significantly increased ATF6 protein levels in mouse granulosa cells. In the meantime, a recombinant plasmid was used to deplete ATF6 successfully using short hairpin RNA-mediated interference technology, which was verified at both the mRNA and protein levels. Flow cytometry and TUNEL assay analysis indicated that ATF6 depletion decreased apoptosis and arrested the S phase of the cell cycle in mouse granulosa cells. Consistent with these results, ,, ,, ,, and mRNA expression decreased, whereas and mRNA expression increased. Interestingly, ATF6 knockdown obviously increased progesterone and estradiol production in mouse granulosa cells. () mRNA levels were downregulated, whereas ,, and mRNA levels were upregulated, in keeping with the changes in steroid hormones. Furthermore, ATF6 disruption remarkably increased () expression and decreased (), (), and () expression in mouse granulosa cells, which are proteins crucial for follicular development. But, after treating with tunicamycin, the levels of ,, and increased relatively, whereas expression decreased. Collectively, these results imply that ATF6, as a key player in ER stress signaling, may regulate apoptosis, the cell cycle, steroid hormone synthesis, and other modulators related to folliculogenesis in mouse granulosa cells, which may indirectly be involved in the development, ovulation, and atresia of ovarian follicles by affecting the physiological function of granulosa cells. The present study extends our understanding and provides new insights into the physiological significance of ATF6, a key signal transducer of ER stress, in ovarian granulosa cells.

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Section: Discussionmentioning

confidence: 63%

ATF6 knockdown decreases apoptosis, arrests the S phase of the cell cycle, and increases steroid hormone production in mouse granulosa cells

Xiong

Chen

Lin

et al. 2017

American Journal of Physiology-Cell Physiology

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“…Moreover, there is no information about the FF composition of Mediterranean water buffalo farmed in Italy. The existing studies on FF composition in buffalo reported variability in relation to the phase of ovarian cycle [12] reproductive acyclicity [8,14] and follicles size [2]. Ovaries of buffalo cows were collected at slaughterhouse during the breeding season.…”

Section: Discussionmentioning

confidence: 99%

“…In buffalo, FF composition has been demonstrated to be influences by the phase of the ovarian cycle [12], though in another study no differences were found between FF collected in luteal and follicular phase [13]. Factors affecting FF composition are: reproductive acyclicity [8,14] and above all follicular dimension. Nandi et al reported that glucose and lactate concentration in FF are affected by follicles size [2].…”

Section: Introductionmentioning

confidence: 93%

Biochemical and Fatty Acids Composition of Water Buffalo (Bubalus Bubalis) Follicular Fluid

et al. 2014

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Aim of this study was to characterize the biochemical and fatty acids composition of follicular fluid collected from follicles of different sizes and in different phases of ovarian cycle in water buffalo farmed in Italy. Ovaries were collected at slaughterhouse during the breeding season; follicular fluid was aspirated dividing samples in small and large follicles (< 6 mm and > 6 mm respectively) and in luteal and follicular phase. Biochemical analysis and gaschromatography were performed. Biochemical and fatty acids composition were greatly influenced by both follicular dimension and phase of ovarian cycle. Biochemical composition and its variations were in agreement with previously study conducted in buffalo and other species. This is the first report of the fatty acids composition of buffalo follicular fluid. Twenty-two fatty acids were identified in follicular fluid; nine were saturated fatty acids, six monounsatured fatty acids and seven polyunsatured fatty acids. The most dominant fatty acids were linoleic acid, oleic acid, palmitic acid, stearic acid and arachidonic acid. All the identified fatty acids concentrations vary at least because of follicle dimension or phase, with the exception of -linoleic acid and arachidonic acid which concentrations remain stable in all classes.

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“…It has been proposed that attaining a particular diameter is not the sole determinant of ovulation, whereas, acquisition of both morphological and functional dominance is important in buffalo (Khan and Das ; Khan et al. ). Therefore, in dairy buffalo, the assessment of amount of blood flow of POF can be a reliable indicator of its functional dominance.…”

Section: Discussionmentioning

confidence: 99%

Correlation of Blood Flow of the Preovulatory Follicle to its Diameter and Endocrine Profile in Dairy Buffalo

Varughese

Brar

Honparkhe

et al. 2013

Reprod Domestic Animals

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Blood flow of the preovulatory follicle (POF) wall can be used as a predictor of the quality of POF. Our aim was to determine the correlation of blood flow of POF with the POF diameter, and intra-follicular and plasma concentrations of Insulin-like Growth Factor-I (IGF-1) and oestradiol in dairy buffalo. Nine Murrah buffalo subjected to an ovulation synchronization protocol (Ovsynch) were assessed on day 10 of the protocol for diameter and blood flow of POF, followed by the aspiration of follicle fluid. Prior to follicular aspiration, blood samples were obtained from jugular vein for estimation of IGF-1 and oestradiol. The vascularity of POF was determined (Range: 250-967 pixel(2) ) along with intra-follicular and plasma concentration of IGF-1 (Range: 9.3-31.8 ng/ml and 14.7-29.7 ng/ml respectively) and oestradiol (Range: 124.2-447.9 ng/ml and 0.25-1.05 ng/ml respectively). Diameter of the POF was weakly correlated (r = 0.21, p < 0.01) with blood flow to it. As compared to POF diameter, the blood flow of POF had greater positive correlation with intra-follicular and plasma concentrations of hormones (IGF-1 and oestradiol). A strong positive correlation was recorded between intra-follicular IGF-1 and oestradiol. Also, plasma concentrations of oestradiol and progesterone were negatively correlated In brief, assessment of the blood flow of the POF is a non-invasive and reliable indicator of its functional competence as compared to the POF diameter.

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Alterations in follicular fluid estradiol, progesterone and insulin concentrations during ovarian acyclicity in water buffalo (Bubalus bubalis)

Cited by 18 publications

References 39 publications

ATF6 knockdown decreases apoptosis, arrests the S phase of the cell cycle, and increases steroid hormone production in mouse granulosa cells

ATF6 knockdown decreases apoptosis, arrests the S phase of the cell cycle, and increases steroid hormone production in mouse granulosa cells

Biochemical and Fatty Acids Composition of Water Buffalo (Bubalus Bubalis) Follicular Fluid

Correlation of Blood Flow of the Preovulatory Follicle to its Diameter and Endocrine Profile in Dairy Buffalo

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