Biochemical and Fatty Acids Composition of Water Buffalo (Bubalus Bubalis) Follicular Fluid

Vencato, J.; Badon, Tamara; Bedin, Silvia; Cogo, Paola; Simonato, Manuela; Stelletta, Calogero

doi:10.6000/1927-520x.2014.03.03.3

“…The Glu concentration increased as the follicle size increased in both the breeding periods in concurrence with the observations of Arshad et al [22]. In the present study, the Glu concentration was higher in all the three follicular categories in LB than HB period.…”

Section: Discussionsupporting

confidence: 80%

Effect of Physico-Biochemical Characteristics of Follicles on Quality and In Vitro Maturation of Bubaline Oocytes

Satheshkumar¹,

Priya²

2016

JFIV Reprod Med Genet

0

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The physico-biochemical properties of follicles and its plausible effect on quality and maturational competency of oocytes during the different breeding periods of the year was studied in buffaloes (Bubalus bubalis). Ovaries were collected from sexually mature buffaloes slaughtered at abattoir during high breeding (December-February; HB) and low breeding (April-July; LB) periods of the year. The surface antral follicles were categorized as small (SF; < 4 mm), medium (MF; 4-9 mm) and large (LF; >9 mm) follicles. The follicular fluid (FF) was aspirated from follicles of different categories and the recovered oocytes were assessed for their quality. The good quality oocytes were cultured in vitro to assess the maturational competency. The glucose (Glu), total proteins (TP) and triglycerides (Tgl) concentrations of FF were analysed and correlated with the oocyte quality and maturation capacity. Among the follicular categories, significantly (P<0.01) more percentage of good quality oocytes with high maturation rate was recovered from MFs and vice versa for oocytes from LFs in both the periods. The mean percentage of good quality oocytes and the mean percentage of oocytes that reached the MII stage was significantly (P<0.01) higher during HB than LB period. The Glu concentration increased as the follicle size increased, while the TP concentration remains similar in all follicular categories but the Tgl concentrations were significantly higher in SFs. In general, the concentrations of these three biochemical components, with significance to Tgl, were found to be increased in the FF during the LB period correlating with the deteriorated oocyte quality and developmental properties. This might mean that metabolic activities were less intensive resulting in lower consumption of these components by the follicular cells and oocytes during the LB than HB period leading to fall in fertility levels during the former period.

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“…Buffaloes have low reproductive performances caused by silent estrus, seasonal anestrous, delayed puberty, late post-partum conception, and a long calving interval. Buffalo oocytes collected from large antral follicles during the luteal phase are less competent due to the presence of different fatty acids in follicular fluid [2]. Due to low response to multiple ovulation and embryo transfer (MOET), in vitro embryo production (IVEP) technology is important in buffaloes.…”

Section: Introductionmentioning

confidence: 99%

Effect of Polyvinylpyrrolidone on Vitrification of Buffalo (Bubalus bubalis) Oocytes

Bari¹,

Islam²,

Alam³

et al. 2020

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Vitrification, a method of rapid cooling, is an alternate cryopreservation method of oocytes and embryos. The present study was aimed to examine the effect of polyvinylpyrrolidone (PVP) on vitrification of buffalo oocytes. Cumulus oocyte complexes (COCs) with fully grown oocytes (120-130 µm in diameter) were aspirated from slaughtered buffalo ovaries for vitrification. COCs were treated with equilibration solution at room temperature for 5 min and then transferred to a vitrification solution for 1 min. Then the COCs were submerged into liquid nitrogen (-196C) for a while using cryotops. The COCs were thawed, diluted, and washed in a washing solution for 5 min, respectively. Vitrified oocytes were incubated for in vitro maturation (IVM) at 38.5C under an atmosphere of 5% CO2 in the air for 24 hrs. Cumulus cells surrounding the oocytes were removed mechanically, oocytes were fixed in acetic acid and ethanol, and stained with aceto-orcein to examine the meiotic stages of oocytes. The numbers of morphologically normal oocytes after vitrification were higher in 5% PVP than 0 and 10% PVP groups. A proportion of oocytes treated with 5% PVP reached the metaphase II (MII) stage while none of the oocytes from 0% and 10% PVP groupsdeveloped beyond anaphase I and metaphase I (MI) stages, respectively. These results show that PVP can be used as a cryoprotectant for the vitrification of buffalo oocytes.

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Effect of Polyvinylpyrrolidone on Vitrification of Buffalo (Bubalus bubalis) Oocytes

Bari¹,

Islam²,

Alam³

et al. 2020

View full text Add to dashboard Cite

Vitrification, a method of rapid cooling, is an alternate cryopreservation method of oocytes and embryos. The present study was aimed to examine the effect of polyvinylpyrrolidone (PVP) on vitrification of buffalo oocytes. Cumulus oocyte complexes (COCs) with fully grown oocytes (120-130 µm in diameter) were aspirated from slaughtered buffalo ovaries for vitrification. COCs were treated with equilibration solution at room temperature for 5 min and then transferred to a vitrification solution for 1 min. Then the COCs were submerged into liquid nitrogen (-196C) for a while using cryotops. The COCs were thawed, diluted, and washed in a washing solution for 5 min, respectively. Vitrified oocytes were incubated for in vitro maturation (IVM) at 38.5C under an atmosphere of 5% CO2 in the air for 24 hrs. Cumulus cells surrounding the oocytes were removed mechanically, oocytes were fixed in acetic acid and ethanol, and stained with aceto-orcein to examine the meiotic stages of oocytes. The numbers of morphologically normal oocytes after vitrification were higher in 5% PVP than 0 and 10% PVP groups. A proportion of oocytes treated with 5% PVP reached the metaphase II (MII) stage while none of the oocytes from 0% and 10% PVP groupsdeveloped beyond anaphase I and metaphase I (MI) stages, respectively. These results show that PVP can be used as a cryoprotectant for the vitrification of buffalo oocytes.

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Biochemical and Fatty Acids Composition of Water Buffalo (Bubalus Bubalis) Follicular Fluid

Cited by 3 publications

References 25 publications

Effect of Physico-Biochemical Characteristics of Follicles on Quality and In Vitro Maturation of Bubaline Oocytes

Effect of Physico-Biochemical Characteristics of Follicles on Quality and In Vitro Maturation of Bubaline Oocytes

Effect of Polyvinylpyrrolidone on Vitrification of Buffalo (Bubalus bubalis) Oocytes

Effect of Polyvinylpyrrolidone on Vitrification of Buffalo (Bubalus bubalis) Oocytes

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