Alterations in cyclin D1 expression in esophageal squamous cell carcinoma in the Indian population

Abstract

Alterations in at least one of the components of the p16/cyclin D1/pRb pathway in majority of the 70 patients analysed herein, and concomitant alterations in all the three proteins in 19 patients (35%) underscore the critical role of this pathway in esophageal tumourigenesis. The results of the present study taken together with our previous findings on p16 and pRb alterations in ESCCs suggest that these alterations are not mutually exclusive and may cooperatively provide greater tumour growth advantage. The prognostic significance of alterations in the expression of these components cyclin D1, p16, and pRb remains to be established in a larger cohort.

Epidermal growth factor receptor and cyclin D1 are independently amplified and overexpressed in esophageal squamous cell carcinoma

“…The gene copy numbers were dichotomized as low (FISH patterns BD+BT) and high (FISH patterns BP+GA) copy numbers per cell. The protein expression was dichotomized in low level (IHC negative for HER-2 and CCND1; score £ 300 for EGFR) and high level of expression (IHC positive for HER-2 and CCND1; score >300 for EGFR) by various techniques ranged from 12 to 21% Mathew et al 2001). EGFR protein was commonly expressed for a high level (49%) in this series and this incidence rate falls within the wide range of 19-83% of ESCC overexpressing patients reported previously (Wang et al 1999;Torzewski et al 1997).…”

“…Score 0 is a negative result with no staining or Low level of protein expression=IHC negative for HER-2 and CCND1, and score 0-300 for EGFR; high level of expression=IHC positive for HER-2 and CCND1, and score >300 for EGFR <10% of cells with nuclear staining; score 1 represents a positive-low tumor with 10-20% stained cells; score 2 is a positive-intermediate tumor with 20-40% stained cells; and score 3 is a positive-high tumor with >40% stained cells. Scores 1-3 represented CCND1 protein overexpression (Mathew et al 2001). …”

“…Only scores 2+ and 3+ were considered to represent protein overexpression. For CCND1, a four-tiered scale following previous publications was applied (Anonymous 2001;Mathew et al 2001). Score 0 is a negative result with no staining or Low level of protein expression=IHC negative for HER-2 and CCND1, and score 0-300 for EGFR; high level of expression=IHC positive for HER-2 and CCND1, and score >300 for EGFR <10% of cells with nuclear staining; score 1 represents a positive-low tumor with 10-20% stained cells; score 2 is a positive-intermediate tumor with 20-40% stained cells; and score 3 is a positive-high tumor with >40% stained cells.…”

Clinicopathological significance of pRb2/p130 expression in squamous cell carcinoma of the esophagus

“…Some cell-cycle related proteins have been suggested as being possible biological markers for differentiation in SCCs of the esophagus (Ohashi et al1997;Hu et al 2001;Mathew et al 2001;Shibata et al 2001). In addition, in the current study, pRb2/p130 expression was significantly more frequent in well-differentiated SCCs than in moderately or poorly differentiated SCCs of the esophagus, which was consistent with the previous investigation referring to the significant correlation of pRb2/p130 expression with frequent incidence of well-differentiated SCC of the head and neck.…”

Cyclin D1 and P16 expression in recurrent nasopharyngeal carcinoma

“…Several investigators reported association between over-expression of the proto-oncogene cyclin D1 with worse prognosis in terms of shorter survival and increased incidence of recurrence [14][15][16]. This poor prognosis in tumors over-expressing cyclin D1 was found to be independent of other clinicopathological parameters such as tumor stage, nodal or distant metastasis, or degree of differentiation [14][15][16]. Other studies however either failed to show this association between over-expression of cyclin D1 and poor clinical outcome [17] or found a reverse association between over-expression of cyclin D1 and poor prognosis.…”