Alterations in cecal microbiota and intestinal barrier function of laying hens fed on fluoride supplemented diets

Miao, Liping; Gong, Ye; Li, Huaiyu; Xie, Chang; Xu, Qianqian; Dong, Xin; Elwan, Hamada A. M.; Zou, Xiaoting

doi:10.1016/j.ecoenv.2020.110372

Cited by 30 publications

(15 citation statements)

References 44 publications

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“…The detailed processes of total RNA extraction and complementary DNA (cDNA) synthesis are as described previously (Miao et al 2020(Miao et al, 2020 ). The gene expressions of ZO-1, ZO-2, claudin-1, claudin-4, and occludin in jejunum and ileum were determined on a real-time polymerase chain reaction (PCR) system (ABI 7500; Applied Biosystems, Foster City, CA, USA) following the protocol of SYBR qPCR Master Mix kit (Vazyme Biotech Co., Ltd, Nanjing, Jiangsu, China).…”

Section: Real-time Pcrmentioning

confidence: 99%

Effects of coated sodium butyrate on production performance, egg quality, serum biochemistry, digestive enzyme activity, and intestinal health of laying hens

Miao

Zhou

et al. 2021

Italian Journal of Animal Science

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The present study was conducted to investigate the effects of coated sodium butyrate (CSB) on production performance, egg quality, serum biochemistry, digestive enzyme activity, intestinal health of laying hens. 720 hens at 52 weeks were assigned in five groups including basal diet (control) and a basal diet supplemented with 250, 500, 750, and 1000 mg/kg CSB for 8 weeks. The results showed that the 500 mg/kg CSB group increased laying rate but 500 and 750 mg/kg groups decreased feed conversion ratio (p < .05). At the fourth week, dietary CSB at more than or equal to 500 mg/kg level increased the Haugh unit (p < .01); At the eighth week, 500 and 1000 mg/kg CSB groups improved the eggshell strength, Haught unit and albumen height (p < .05). Dietary CSB administration except for the 250 mg/kg group increased the serum albumin and calcium and decreased the triglyceride content (p < .05). 500 and 750 mg/kg CSB groups increased the activities of trypsin and amylase in the pancreas and duodenum (p < .05). The groups of 500 and 750 mg/kg increased the villus height and villus height to crypt depth ratio (V/C) in the jejunum and ileum (p < .05). In the jejunum, at more than or equal to 500 mg/ kg CSB groups increased the expressions of ZO-1 and Occludin (p < .05); In the ileum, 500 and 750 mg/kg CSB groups increased the expressions of claudin-1 and occludin and ZO-2 (p < .05). Conclusively, dietary supplementation of CSB can improve egg production, egg quality, digestive enzyme activity and present a positive effect on improving villi and intestinal mechanical barrier function. HIGHLIGHTSA significant improvement in production performance and egg quality was observed in birds fed diet with coated sodium butyrate. Administration of coated sodium butyrate enhanced protein and mineral availability and lipid metabolism of serum. Supplementation of coated sodium butyrate presented a positive impact on regulating intestinal health.

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Section: Real-time Pcrmentioning

confidence: 99%

Effects of coated sodium butyrate on production performance, egg quality, serum biochemistry, digestive enzyme activity, and intestinal health of laying hens

Miao

Zhou

et al. 2021

Italian Journal of Animal Science

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“…12 The symbiotic microbes in the intestine constitute the intestinal microbial barrier, which not only restrains the proliferation of pathogens by competing for limited nutrients and releasing antimicrobial productions, but also supplies the host with several physiological processes. 13 Metabolomics is an emerging investigative tool that uses highthroughput techniques to quantitate small-molecule metabolites in biological samples. 14 The identification and comprehensive analysis of these metabolites can provide information about early biochemical changes in animals, allowing the opportunity to create biomarkers that could result in interventions or give significant information about the components of various clinical conditions.…”

Section: Introductionmentioning

confidence: 99%

Rhamnolipids enhance growth performance by improving the immunity, intestinal barrier function, and metabolome composition in broilers

Zhang¹,

Zhang²,

Liu

et al. 2021

J Sci Food Agric

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BACKGROUND: Rhamnolipids (RLS), well known as glycolipid biosurfactants, display low toxicity, high biodegradability, and strong antibacterial properties. This study was carried out to evaluate the use of RLS supplementation as a substitute for antibiotics, and particularly to evaluate its effects on growth performance, immunity, intestinal barrier function, and metabolome composition in broilers. RESULTS:The RLS treatment improved the growth performance, immunity, and intestinal barrier function in broilers. The 16S rRNA sequencing revealed that the genus Alistipes was the dominant genus in broilers treated by RLS. An ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based metabolomic analysis indicated that the sphingolipid metabolism, glycine, serine, and threonine metabolism, the gycerophospholipid metabolism, and the tryptophan metabolism were changed in broilers that were treated with RLS.CONCLUSION: L-Tryptophan may be the medium for RLS to regulate the growth and physiological metabolism. Rhamnolipids can be used as a potential alternative to antibiotics, with similar functions to antibiotics in the diet of broilers. The optimal level of supplemented RLS in the diet was 1000 mg kg −1 .

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“…Intestinal flora have heterogeneous microbial communities in different segments of the gut in animals and the caecum, an ideal habitat for a variety of microorganisms, and is the most abundant part of the intestinal flora [ 22 ]. In clinical studies, by comparing the contents of the human caecum with faeces, Marteau et al found a significant difference in the composition of bacteria [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Involvement of mucosal flora and enterochromaffin cells of the caecum and descending colon in diarrhoea-predominant irritable bowel syndrome

et al. 2021

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Background Accumulating evidence supports the pivotal role of intestinal flora in irritable bowel syndrome (IBS). Serotonin synthesis by enterochromaffin (EC) cells is influenced by the gut microbiota and has been reported to have an interaction with IBS. The comparison between the microbiota of the caecal and colonic mucosa in IBS has rarely been studied. The aim of this study was to investigate the relationship between the gut microbiota, EC cells in caecum and descending colon, and diarrhoea-predominant IBS (IBS-D) symptoms. Results A total of 22 IBS-D patients and 22 healthy controls (HCs) were enrolled in our study. Hamilton anxiety (HAM-A) and Hamilton depression (HAM-D) grades increased significantly in IBS-D patients. In addition, the frequency of defecation in IBS-D patients was higher than that in HCs. Among the preponderant bacterial genera, the relative abundance of the Ruminococcus_torques_ group increased in IBS-D patients in caecum samples while Raoultella and Fusobacterium were less abundant. In the descending colon, the abundance of the Ruminococcus_torques_group and Dorea increased in IBS-D patients and Fusobacterium decreased. No difference was observed between the descending colon and caecum in regards to the mucosal-associated microbiota. The number of EC cells in the caecum of IBS-D patients was higher than in HCs and the expression of TPH1 was higher in IBS-D patients both in the caecum and in the descending colon both at the mRNA and protein level. Correlation analysis showed that the Ruminococcus_torques_group was positively associated with HAM-A, HAM-D, EC cell number, IBS-SSS, degree of abdominal pain, frequency of abdominal pain and frequency of defecation. The abundance of Dorea was positively associated with EC cell number, IBS-SSS, HAM-A, HAM-D and frequency of abdominal pain. Conclusions EC cell numbers increased in IBS-D patients and the expression of TPH1 was higher than in HCs. The Ruminococcus torques group and Dorea furthermore seem like promising targets for future research into the treatment of IBS-D patients.

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Alterations in cecal microbiota and intestinal barrier function of laying hens fed on fluoride supplemented diets

Cited by 30 publications

References 44 publications

Effects of coated sodium butyrate on production performance, egg quality, serum biochemistry, digestive enzyme activity, and intestinal health of laying hens

Effects of coated sodium butyrate on production performance, egg quality, serum biochemistry, digestive enzyme activity, and intestinal health of laying hens

Rhamnolipids enhance growth performance by improving the immunity, intestinal barrier function, and metabolome composition in broilers

Involvement of mucosal flora and enterochromaffin cells of the caecum and descending colon in diarrhoea-predominant irritable bowel syndrome

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