Alteration of the miRNA expression profile in male porcine anterior pituitary cells in response to GHRH and CST and analysis of the potential roles for miRNAs in regulating GH

Qi, Qien; Xi, Qianyun; Ye, Rui-Song; Chen, Ting; Cheng, Xiao; Li, Chao-Yun; Zhu, Xiaotong; Shu, Gang; Wang, Lina; Jiang, Qingyan; Zhang, Yongliang

doi:10.1016/j.ghir.2014.12.002

Cited by 20 publications

(16 citation statements)

References 44 publications

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“…A total of 416 miRNAs were obtained from samples, 343 of which are known miRNAs and 73 are newly predicted miRNAs. Our research group has nished revealing the expression of miRNAs in porcine anterior pituitary cells and found that miRNAs could regulate the hormone secretion from the anterior pituitary [43,108,109]. Interestingly, we found most of top 20 miRNAs like miR-7, miR-375, let-7a, let-7c, miR-26a, miR-30a, let-7g, miR-30d, miR-127, miR-151, miR-21, miR-149, miR-99a, miR-143 in exosome were also highly expressed in the porcine pituitary [110].…”

Section: Discussionmentioning

confidence: 99%

An exploration of non-coding RNA in exosomes delivered by swine anterior pituitary

Xiong

Zhang

Zeng

et al. 2020

Preprint

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Background: The anterior pituitary is a key endocrine organ both in animal and human being drawing much concern. Exosomes are extracellular secretory vesicles carrying proteins, lipids and small RNAs. Previous studies have demonstrated that they had regulatory function both physiologically and pathologically. However, information on exosomes from anterior pituitary remains unknown.Results: In this study, we separated and identified exosomes from anterior pituitary of Duroc swine model for the first time. Total RNA was extracted and RNA-seq was performed, followed by a comprehensive analysis of miRNAs, lncRNAs and circRNAs. Resultantly, we obtained 343 known miRNAs and 73 novel miRNAs, 15545 lncRNAs and 494 circRNAs. Furthermore, GO and KEGG enrichment analysis showed that the ncRNAs in exosomes may participate in regulating intracellular signal transduction, cellular component organization or biogenesis, small molecule binding, transferase activity. The cross-talk between them also suggested that they may play an important role in signaling process and the biological regulation.Conclusions: This work firstly provides ncRNAs data in anterior pituitary exosomes from duroc swine breed. These results may serve as a fundamental resource for exploring the detailed functions of exosomes from anterior pituitary.

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Section: Discussionmentioning

confidence: 99%

An exploration of non-coding RNA in exosomes delivered by swine anterior pituitary

Xiong

Zhang

Zeng

et al. 2020

Preprint

Self Cite

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“…More recently, miRNA expression profiles were analyzed in the rat pituitary from postnatal development throughout the entire lifespan showing that specific mi-RNAs are differentially expressed during pituitary development [43]. In addition, there is evidence that GH synthesis and release are also regulated by miRNAs in mammals [44], as documented below.…”

Section: Mirnas Regulating the Hypothalamus-pituitary And Gh/igf-i Axesmentioning

confidence: 93%

“…Among these miRNAs, miR-361-5p and miR-127 were regulated in opposite ways under the two treatments, suggesting a potential role in GH regulation which still needs further investigation. Interestingly, let-7c, which was upregulated under CST treatment, negatively regulated GH production and release, directly targeting GH1 and GHRH [44] (Table 1). DOI: 10.1159/000504669…”

Section: Mirnas Gh and Ghrmentioning

confidence: 99%

The Role of MicroRNAs in Influencing Body Growth and Development

et al. 2020

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Body growth and development are regulated among others by genetic and epigenetic factors. MicroRNAs (miRNAs) are epigenetic regulators of gene expression that act at the post-transcriptional level, thereby exerting a strong influence on regulatory gene networks. Increasing studies suggest the importance of miRNAs in the regulation of the growth plate and growth hormone (GH)-insulin-like growth factor (IGF) axis during the life course in a broad spectrum of animal species, contributing to longitudinal growth. This review summarizes the role of miRNAs in regulating growth in different in vitro and in vivo models acting on GH, GH receptor (GHR), IGFs, and IGF1R genes besides current knowledge in humans, and highlights that this regulatory system is of importance for growth.

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“…miRs are evolutionarily conserved, small single-stranded molecules (20-24 nucleotides), that have been postulated to regulate the expression of approximately 50% of the genes in a cell at the post-transcriptional level [62,63]. In contrast to other endogenous small RNAs, miRNAs derive from transcripts forming distinctive hairpin structures [64].…”

Section: Micrornas and Their Biological Rolesmentioning

confidence: 99%