ALTERATION OF GLUCOSINOLATE CONTENT, PATTERN, AND MUTAGENICITY OF CABBAGE (BRASSICA OLERACEA) GROWN ON MUNICIPAL SEWAGE SLUDGE—AMENDED SOIL

Abstract

Cabbage (Brassica oleracea var. capitata) was grown on soil (control) or m u n i c i p l sewage sludge-treated plots. The cabbage was freeze-dried and analyzed for glucosinolate content and pattern. Results showed that cabbage grown o n sludge-amended soil contained only half the glucosinolate content of cabbage grown o n the control plot. The pattern of individual glucosinolates present was also altered. The sludge-grown cabbage glucosinolate extract was shown to be mutagenic in the nonactivated test system of S. t y p h i m u r i u m TA 100. Eztracts of both sludgegrown and control cabbage were shown to enhance the mutagenicity on a dose-response basis of aJEatoxin B,, benzo(a)pyrene, and captan. tion of this sludge to cropland (Furr et al. 1976;Babish et al. 1979Babish et al. ,1981Boyd et al. 1982a;Lisk et al. 1982;Telford et al. 1982).

Processing ofcrambe abyssinica seed in commercial extraction facilities

“…The intensity of color developed in the TesTape ~ gave an indication of the glucosinolate content of the sample. Where no color developed (enzyme inactive), addition of 10 mg of purified mustard mynosinase (13,21) to the meal slurry gave a quick check on residual glucosinolate as glucose was released. Frequent in-plant checks using this technique provided insight into the fate of epi-progoitrin (I) as processing advanced from stage to stage.…”