Analysis of oil and meal from<i>Lesquerella fendleri</i> seed

Carlson, K. Douglas; Chaudhry, A.; Bagby, M. O.

doi:10.1007/bf02638957

Cited by 44 publications

(23 citation statements)

References 8 publications

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“…Another member of the Brassicaceae, Lesquerella fendleri (Gray) S. Wats., commonly referred to as desert mustard, produces seed oil high in hydroxy fatty acids (Carlson et al, 1990;Skarjinskaia 2003 ). Oils high in hydroxy fatty acids can replace castor oil, which is used extensively in industrial applications including cosmetics, plastics and coatings (Redd et al, 1997;Dykinga 1999) The quality of plant oils and their industrial value are determined by fatty acid content and composition (Scarth and Tang 2006).…”

Section: Introductionmentioning

confidence: 99%

Abscisic acid metabolism and lipid accumulation of a cell suspension culture of Lesquerella fendleri

Kharenko

Zaharia

Giblin

et al. 2010

Plant Cell Tiss Organ Cult

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Lesquerella fendleri (commonly known as "Fendler's bladderpod" or "yellowtop") is a member of the Brassicaceae and is an important seed oil-producing plant. The lipid profile of L. fendleri seed indicates potential for producing a high quality replacement for castor oil. In this work, characterization of the lipid content of a suspension cell culture, derived from seedlings of L. fendleri, is provided. Under the described suspension cell culture conditions, 16:0, 18:1 9, 18:2 9, 12 and 18:3 9, 12, 15 fatty acids were found to accumulate in the cells, while 16:0, 26:0 and 28:0 fatty acids were predominant in the culture medium. Subsequently, the effect of application of abscisic acid (ABA), which modulates lipid accumulation, was assessed.

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Section: Introductionmentioning

confidence: 99%

Abscisic acid metabolism and lipid accumulation of a cell suspension culture of Lesquerella fendleri

Kharenko

Zaharia

Giblin

et al. 2010

Plant Cell Tiss Organ Cult

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“…The seed also contains valuable polysaccharide gums (2) and 22% protein with favorable amino acid composition (3). The status of lesquerella as an industrial crop was reviewed (4).…”

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Lesquerella fendleri protein fractionation and characterization

Hojilla-Evangelista

2005

J Americ Oil Chem Soc

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Lesquerella fendleri is a promising new crop whose seed contains hydroxy FA TG with potential industrial uses as well as substantial amounts of valuable gums. The defatted L. fendleri seeds also contain more than 30% protein. The objective of this study is to process and characterize this protein component for possible future uses in food. Hexane-defatted seed has more than 30% protein content. Defatted lesquerella meal was extracted sequentially with 0.5 M sodium chloride (2×), water, 70% ethanol, and 0.1 N sodium hydroxide (2×). Each sodium chloride extract was dialyzed against deionized water and centrifuged to separate the water-soluble fraction (albumin) from the salt-soluble fraction (globulin) before freeze-drying. The ethanol extract and the neutralized sodium hydroxide extracts (glutelin) were dialyzed against water and freeze-dried. Albumin had the highest proportion of lysine and sulfur amino acids per 16 g nitrogen among all the fractions analyzed. Glutelin and globulin accounted for the highest amount of protein nitrogen. SDS-PAGE of the reduced albumin, globulin, and glutelin showed the presence of several protein bands with M.W. ranging from 7 to 98 kDa. Nitrogen solubility of defatted lesquerella meal from pH 2 to 12 indicated a solubility minimum of 15% around pH 4.2 and a solubility of 75% at pH 11.5. Nonprotein nitrogen of defatted meal was 12% of total nitrogen. Defatted lesquerella meal has the potential for food use based on good nitrogen solubility and good amino acid composition.Paper no. J10936 in JAOCS 82, 53-56 (January 2005). KEY WORDS:Amino acid composition, Lesquerella, lesquerella protein, nitrogen solubility, nonprotein nitrogen, protein fractionation, SDS-PAGE.Lesquerella fendleri is a promising new crop whose seed contains hydroxy FA TG with potential uses in cosmetics, paint, coatings, lubricants, and grease (1). The seed also contains valuable polysaccharide gums (2) and 22% protein with favorable amino acid composition (3). The status of lesquerella as an industrial crop was reviewed (4). Forty acres (16.2 ha) of lesquerella was grown in the southwestern United States in 2003. In present practice, the lesquerella seed is being processed for oil, and it is essential to find an economic outlet for the defatted meal. There is increasing interest among vegetarians and health-conscious people to consume protein-enriched foods from plant sources, which have no cholesterol and a low saturated fat content. Protein accounts for 30% of defatted lesquerella meal (3), but no information is available on the individual proteins of lesquerella. The historical way to fractionate plant protein is to separate protein into water-soluble albumin, salt-soluble globulin, ethanol-soluble prolamin, and alkali-soluble glutelin. More basic information on lesquerella protein fractions is necessary to better utilize the protein. The objective of this paper is to study the fractionation of protein into albumin, globulin, and glutelin fractions and to determine nitrogen solubility and nonprotein nitrogen o...

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“…Along with revenue from the HFAs, co-products of seed oil production in Lesquerella have the potential to significantly increase the return generated from the crop, which could lead to greater utilization. For example, profitable outlets exist for the seed meal of Lesquerella because it has favorable nutritional qualities (i.e., it is high in protein and has a good amino acid balance with high levels of lysine) that make it desirable for livestock and poultry feed (Miller et al, 1962;Carlson et al, 1990;Roetheli et al, 1991). Gums from the seed coats of several species of Lesquerella are potentially as valuable as the oil, and could be used as thickening or gelling agents in edible and nonedible products, especially in the pharmaceutical industry (Holser et al, 2000).…”

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Evaluation of new Lesquerella and Physaria (Brassicaceae) oilseed germplasm

Salywon

Dierig

Rebman

et al. 2005

American J of Botany

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The seed oil of Lesquerella and the closely related genus Physaria (Brassicaceae) is rich in hydroxy fatty acids (HFAs). HFAs and their derivatives are used to produce a variety of industrial products including lubricants, nylon-11, plastics, drying agents, protective coatings, surfactants, cosmetics, and pharmaceuticals. Lesquerella fendleri is being developed as a new crop for arid regions of the southwestern United States as an alternative source of HFAs. Between 1995 and 2001, 66 accessions from 28 species of Lesquerella were collected in the United States, 33 accessions from four species were collected in Mexico, and 41 accessions from 15 species of Physaria were collected from the southwestern United States. Mean seed mass ranged from 0.54 to 2.30 mg for Lesquerella compared to 1.70 to 5.80 mg for Physaria. Seed oil content ranged from a high of 32.2% in Lesquerella to a high of 35.4% in Physaria. The fatty acid profile of all species of Physaria and most of the lesquerolic-acid-rich species of Lesquerella contained from 30 to 55% lesquerolic acid, although several species contained Ͼ60%. These collections of wild germplasm provide a diverse gene pool that should enhance our breeding program in developing a domestic source of HFAs.

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Analysis of oil and meal fromLesquerella fendleri seed

Cited by 44 publications

References 8 publications

Abscisic acid metabolism and lipid accumulation of a cell suspension culture of Lesquerella fendleri

Abscisic acid metabolism and lipid accumulation of a cell suspension culture of Lesquerella fendleri

Lesquerella fendleri protein fractionation and characterization

Evaluation of new Lesquerella and Physaria (Brassicaceae) oilseed germplasm

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