Alteration of Gills and Liver Histological Structure of Cyprinus carpio L. Exposed to Leachate

Abstract

One of the main problems in the waste management in Indonesia is the treatment of leachate, which mostly dumped to the river This research is aimed to obtain information of histological alteration in gills and liver of C. carpio L. exposed to leachate. Measurements on the water quality parameters comprised water temperature, pH, and dissolved oxygen (DO). This research was conducted by exposing leachate to C. carpio for 96 hours. The concentration of leachate were 0 ppm, 80 ppm, and 100 ppm. Histological preparation were made on the gills and liver using 10% fixative Neutral Buffered Formalin and Ehrlich Hematoxylin-Eosin staining with qualitative observation descriptive analyses for discussion. The result showed that increasing water temperature is directly proportional to the leachate concentration in the aquaria, while the value of pH and DO inversely proportional to the leachate concentration. Damages on the gills with 80 ppm leachate concentrasion were identified as follows: fusion of secondary gill filaments and hyperplasia of epithelial cell, along with karyorrhexis and hydropic degeneration on the liver. Damages on the gills of fishes exposed to leachate with 100 ppm concentrasion were identified as follows: fusion of secondary gill filaments, hyperplasia of epithelial cell, congestion, and edema along with karyorrhexis, hydropic degeneration and melanomacrophage centre (MMC) found on the liver. The results of this study can be used as an overview of the impact of an environmental pollution by leachate as indicated from histological damage to the gills and liver of C. carpio, thus contribute significan information to aquaculture sector and endorse better waste management

Physiological and bacteriological profile of the fish Clarias gariepinus (Siluriformes: Clariidae) chronically exposed to different concentrations of municipal waste leachate in Nigeria

“…This result supports several observations which reported that fish exposed to Cd, Cu, Fe, Pb, Mn and Zn and other heavy metals and contaminants resulted in histopathological changes (Randi, Monserrat, Rodriguez, & Romano, 1996). This result is also similar to the report of Pribadi, Syahidah, Harjanti, and Malini (2017) on Cyprinus carpio exposed to leachate. Severe necrosis observed at higher concentration of leachate might have resulted from the effort to get rid of the toxicant and the failed attempt to regenerate new cells.…”

Histological Alteration of Green Mussel Perna viridis Organs Exposed to Microplastics

“…Lamellar fusion is likely caused by lamella hyperplasia (Carvalho et al, 2020). This is also assumed to indicate cell degeneration and eventually a sign of early necrosis (Pribadi et al, 2017). Hyperplasia is characterized by enlargement of the epithelium due to an increasing number of cells that cause dilation on the lamellae; therefore, lamellar fusions were occurred (Figure 2).…”