Alteration of contractile and regulatory proteins in estrogen-induced hypertrophy of female rabbit bladder

Lin, Alpha Dian-Yu; Levin, Robert M.; Kogan, Barry A.; Whitbeck, Catherine; Leggett, Robert E.; Kearns, Cristin; Mannikarottu, Anita

doi:10.1016/j.urology.2006.08.1094

Cited by 23 publications

(23 citation statements)

References 19 publications

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“…This is in agreement with previous work wherein identification of these proteins has also been demonstrated [25,27,30,31,37,45]. In addition, recent reports demonstrate that ROKβ is partly responsible for the high degree of force maintenance in the detrusor smooth muscle in overactive bladders [30][31][32].…”

Section: Discussionsupporting

confidence: 93%

Comparative pharmacological analysis of Rho-kinase inhibitors and identification of molecular components of Ca2+ sensitization in the rat lower urinary tract

Teixeira

Jin

Priviero

et al. 2007

Biochemical Pharmacology

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We aimed to compare the expression and function of molecular components of the RhoA/Rho-kinase signaling pathway in the contractile responses of detrusor, trigonal and urethral smooth muscle, using selective Rho-kinase inhibitors. Contractility studies and molecular approaches were employed to demonstrate the expression patterns and functional activity of the RhoA/Rho-kinase signaling pathway in the lower urinary tract. Frequency-response curves (1-32 Hz) and concentration-response curves (CRC) to carbachol (CCh, 0.01-30 μM), phenylephrine (PE, 0.01-300 μM) and endothelin-1 (ET-1, 0.01-100 nM) were significantly attenuated (p<0.01) following incubation with the Rhokinase inhibitors H-1152 (0

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Section: Discussionsupporting

confidence: 93%

Comparative pharmacological analysis of Rho-kinase inhibitors and identification of molecular components of Ca2+ sensitization in the rat lower urinary tract

Teixeira

Jin

Priviero

et al. 2007

Biochemical Pharmacology

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“…2 vP and uP in ovx control animals are considerably lower than in E2 treated animals which confirms our previous observation. The increased bladder and urethral pressure are most likely due to increased muscular activity as a higher muscle mass of the detrusor and sphincter and higher blood supply was reported in ovx-E2 in comparison to untreated animals (Aikawa et al, 2003;Lin et al, 2006a;Lin et al, 2006b;Lin et al, 2006c). It appears that the 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 6 phytoestrogens have similar, though weaker effects in the detrusor than in the sphincter apparatus.…”

Section: Discussionmentioning

confidence: 96%

Comparison of urodynamic effects of phytoestrogens equol, puerarin and genistein with these of estradiol 17β in ovariectomized rats

Thielemann

Wuttke

et al. 2010

Experimental Gerontology

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Comparison of urodynamic effects of phytoestrogens equol, puerarin and genistein with these of estradiol 17β in ovariectomized rats. Experimental Gerontology, Elsevier, 2010, 45 (2) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ACCEPTED MANUSCRIPT AbstractWhether urinary incontinence in the postmenopause can be prevented or delayed by estrogens is currently controversially debated. Ovariectomized (ovx) rats have been successfully used as models in the past years but plant derived substances with estrogenic effects in the lower urinary tract have not been studied so far. Therefore we compared the effects of a 3 months lasting oral administration of estradiol 17ß (E2) with those of the phytoestrogens equol, genistein and puerarin. They were then anaesthetized and catheterized with a biluminal catheter having one outlet in the bladder and another in the urethra at the level of the urethral sphincter. Urethral and bladder pressure were recorded during a 240 sec period of retrograde bladder filling (2 x 0.5 ml within 30 s with 1 min filling intermission). Bladder and urethra pressures were highest in the E2>puerarin>equol>genistein treated animals. Phytoestrogen and E2 treatment resulted in consistently higher urethral than the bladder pressures during the filling period and in the filled status whereas bladder often exceeded urethral pressures in ovx controls. In summary, we demonstrate significant improvement of urethral closure mechanism under E2 and phytoestrogen administration that can be assumed to be beneficial for prevention or therapy of postmenopausal urge incontinence.

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“…Chez le lapin, après ovariectomie, l'étude du calendrier mictionnel montre une pollakiurie [30]. Les études en bains d'organes isolés de vessie de lapin et de rat révèlent une hypocontractilité vésicale à toutes les formes de stimulation (stimulation électrique, ATP, carbachol, KCl) [33,37,44,51,52]. Cette hypocontractilité se corrige après traitement par estrogène.…”

Section: Introductionunclassified

“…Inversement, les lapins et rats traités par estrogènes après ovariectomie, ont une contractilité augmentée [33,37,44,51,52]. Parallèlement, il a été rapporté que les lapins traités par estrogènes avaient une augmentation de la relaxation médiée par les récepteurs b2-et b3-adrénergiques, qui pourrait être liée à une augmentation de l'AMPc dans les cellules musculaires lisses du détrusor [53][54][55].…”

Section: Introductionunclassified

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Estrogènes et fonction vésicale

et al. 2007

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L'objectif de cette étude est de décrire l'impact des estrogènes sur la fonction vésicale. Les récepteurs aux estrogènes sont exprimés au niveau du dôme et du trigone vésical et au niveau du système nerveux central et périphérique innervant la vessie.En cas de déprivation estrogénique, il apparaît, au niveau de la paroi vésicale, une atrophie musculaire lisse, une augmentation du collagène et une augmentation du ratio collagène/muscle lisse. L'estrogénothérapie entraîne une hypertrophie musculaire, une augmentation du poids de la vessie, une néo-angiogenèse et une modification de la densité des récepteurs a-adrénergi-ques, muscariniques et purinergiques.Au niveau fonctionnel, ces modifications de structure sont responsables lors d'une déprivation estrogénique d'une hypocontractilité vésicale, et en cas de traitement estrogé-nique, d'une augmentation de la contractilité vésicale.Au niveau de l'innervation vésicale, les estrogènes semblent avoir une action prédominante au niveau de l'innervation afférente et en particulier au niveau des neurones vanilloïdes.Abstract: The objective of this study was to describe the role of estrogens in bladder function. Estrogen receptors have been shown to be expressed in the dome and trigone of the bladder and in the central and peripheral nervous systems.Estrogen deprivation is associated with smooth muscle atrophy of the bladder wall and an increase in collagen levels and the collagen-to-smooth muscle ratio.Estrogens induce muscular hypertrophy, increased bladder weight, angiogenesis and changes in muscarinic, a-adrenergic and purinergic receptor densities.Consequently, these structural changes, induce bladder hypocontractility in the case of estrogen deprivation; estrogen replacement improves bladder contractility. Concerning neurological control, estrogens seem to impact predominantly afferent nerve pathways, especially capsaicin-sensitive afferent fibres.

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Alteration of contractile and regulatory proteins in estrogen-induced hypertrophy of female rabbit bladder

Cited by 23 publications

References 19 publications

Comparative pharmacological analysis of Rho-kinase inhibitors and identification of molecular components of Ca2+ sensitization in the rat lower urinary tract

Comparative pharmacological analysis of Rho-kinase inhibitors and identification of molecular components of Ca2+ sensitization in the rat lower urinary tract

Comparison of urodynamic effects of phytoestrogens equol, puerarin and genistein with these of estradiol 17β in ovariectomized rats

Estrogènes et fonction vésicale

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