Alteration of bacterial adhesion induced by the substrate stiffness

Guégan, C.; Gardères, Johan; Pennec, Gaël Le; Gaillard, Fanny; Faÿ, Fabienne; Linossier, Isabelle; Herry, Jean‐Marie; Fontaine, M.; Réhel, Karine

doi:10.1016/j.colsurfb.2013.10.010

Cited by 72 publications

(69 citation statements)

References 30 publications

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“…D41 occurred on agarose hydrogels that had a storage modulus of 110 kPa than on soft agarose hydrogels that had a storage modulus of 6.6 kPa. 23 Additionally, proteomic profiling indicated that significant phenotypic changes (i.e., differential regulation of key metabolic pathways and an increased synthesis of outer membrane proteins) occurred to the bacteria that adhered to the stiffer versus the softer agarose hydrogels. These studies definitively show that bacteria respond to substrate stiffness or polymer concentration and exhibit phenotypic changes in response to surface association.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Adhesion Is Affected by the Thickness and Stiffness of Poly(ethylene glycol) Hydrogels

Kolewe

Zhu

Mako

et al. 2018

ACS Appl. Mater. Interfaces

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Despite lacking visual, auditory, and olfactory perception, bacteria sense and attach to surfaces. Many factors including, the chemistry, topography, and mechanical properties of a surface, are known to alter bacterial attachment, and in this study, using a library of nine protein-resistant poly(ethylene glycol) (PEG) hydrogels immobilized on glass slides, we demonstrate that the thickness or amount of polymer concentration also matters. Hydrated atomic force microscopy and rheological measurements corroborated that thin (15 μm), medium (40 μm), and thick (150 μm) PEG hydrogels possessed Young’s moduli in three distinct regimes, soft (20 kPa), intermediate (300 kPa), and stiff (1000 kPa). The attachment of two diverse bacteria, flagellated gram-negative Escherichia coli and non-motile gram-positive Staphylococcus aureus was assessed after a 24 h incubation on the nine PEG hydrogels. On the thickest PEG hydrogels (150 μm), E. coli and S. aureus attachment increased with increasing hydrogel stiffness. However, when hydrogel’s thickness was reduced to 15 μm, a substantially greater adhesion of E. coli and S. aureus was observed. Twelve times fewer S. aureus and eight times fewer E. coli adhered to thin-soft hydrogels than to thick-soft hydrogels. Though a full mechanism to explain this behavior is beyond the scope of this paper, we suggest that because the Young’s moduli of thin-soft and thick-soft hydrogels were statistically equivalent, potentially, the very stiff underlying glass slide was causing the thin-soft hydrogels to feel stiffer to the bacteria. These findings suggest a key takeaway design rule; to optimize fouling-resistance, hydrogel coatings should be thick and soft.

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Section: Introductionmentioning

confidence: 99%

Bacterial Adhesion Is Affected by the Thickness and Stiffness of Poly(ethylene glycol) Hydrogels

Kolewe

Zhu

Mako

et al. 2018

ACS Appl. Mater. Interfaces

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“…This acidification causes the initially glassy CH layer to hydrate and swell so that the TOB stored in the PAA layer is able to diffuse into the biofilm and kill bacteria as represented in Figure 1B. Because bacterial adhesion may depend on modulus, 73 the viscous CH layer can further impair bacterial adherence and biofilm formation. In this manuscript, we characterize, the pH-responsive bilayer swelling and collapse, TOB uptake, storage, and release, and antibacterial efficacy of the CH/PAA bilayer.…”

Section: Introductionmentioning

confidence: 99%

Targeted Release of Tobramycin from a pH-Responsive Grafted Bilayer Challenged with S. aureus

Lee¹,

Dastgheyb

Hickok

et al. 2015

Biomacromolecules

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A stimuli-responsive, controlled release bilayer for the prevention of bacterial infection on biomaterials is presented. Drug release is locally controlled by the pH-responsiveness of the bilayer, comprised of an inner poly(acrylic acid) (PAA) monolayer grafted to a biomaterial and cross-linked with an outer chitosan (CH) brush. Tobramycin (TOB) is loaded in the inner PAA in part to minimize bacteria resistance. Because biofilm formation causes a decrease in local pH, TOB is released from PAA and permeates through the CH which is in contact with the biofilm. Antibiotic capacity is controlled by the PAA thickness which depends on PAA brush length and the extent of cross-linking between CH and PAA at the bilayer interface. This TOB-loaded, pH responsive bilayer exhibits significantly enhanced antibacterial activity relative to controls.

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“…The formation and composition of biofilm appear to vary on different surfaces (Aroonsang et al, 2014) and effects of material/surface properties, such as surface charge, hydrophobicity, roughness, topography, and chemistry on bacterial adhesion and biofilm formation have been investigated for many years (Anselme et al, 2010; Badihi Hauslich et al, 2013; Guégan et al, 2014; Perera-Costa et al, 2014; Song & Ren, 2014). These factors may be interrelated, which may explain the inhibition of biofilm formation found on irradiated enamel.…”

Section: Discussionmentioning

confidence: 99%

Effects of CO₂laser irradiation on matrix-rich biofilm development formation–an in vitro study

Zancopé

Dainezi

Nobre‐dos‐Santos

et al. 2016

PeerJ

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BackgroundA carbon dioxide (CO2) laser has been used to morphologically and chemically modify the dental enamel surface as well as to make it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of a CO2 laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2 laser (λ = 10.6 µM).MethodsFor this in vitro study, 96 specimens of bovine enamel were employed, which were divided into two groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by one, three and five days under intermittent cariogenic condition in the irradiated and non-irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and, in fifth day, were evaluated by polysaccharides analysis, quantitative real time Polymerase Chain Reaction (PCR) as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test.ResultsThe mean of log CFU/mL obtained for the one-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to the CO2 laser, CFU/mL and CFU/dry weight in three day was reduced significantly compared with control group. The difference in the genes expression (Glucosyltransferases (gtfB) and Glucan-binding protein (gbpB)) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with the CO2 laser. Similar morphology was also visible with both treatments; however, the irradiated group revealed evidence of melting and fusion in the specimens.ConclusionIn conclusion, CO2 laser irradiation modifies the energy surface and disrupts the initial biofilm formation.

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Alteration of bacterial adhesion induced by the substrate stiffness

Cited by 72 publications

References 30 publications

Bacterial Adhesion Is Affected by the Thickness and Stiffness of Poly(ethylene glycol) Hydrogels

Bacterial Adhesion Is Affected by the Thickness and Stiffness of Poly(ethylene glycol) Hydrogels

Targeted Release of Tobramycin from a pH-Responsive Grafted Bilayer Challenged with S. aureus

Effects of CO₂laser irradiation on matrix-rich biofilm development formation–an in vitro study

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Alteration of bacterial adhesion induced by the substrate stiffness

Cited by 72 publications

References 30 publications

Bacterial Adhesion Is Affected by the Thickness and Stiffness of Poly(ethylene glycol) Hydrogels

Bacterial Adhesion Is Affected by the Thickness and Stiffness of Poly(ethylene glycol) Hydrogels

Targeted Release of Tobramycin from a pH-Responsive Grafted Bilayer Challenged with S. aureus

Effects of CO2laser irradiation on matrix-rich biofilm development formation–an in vitro study

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Effects of CO₂laser irradiation on matrix-rich biofilm development formation–an in vitro study