Alteration in the expression of the renin-angiotensin system in the myocardium of mice conceived by in vitro fertilization†

Wang, Qijing; Zhang, Yue; Fang, Le; Wang, Ning; Zhang, Fan; Luo, Yuqin; Lou, Yiyun; Hu, Minhao; Wang, Liya; Thurston, L.M.; Xu, Xiangrong; Jin, Fan

doi:10.1093/biolre/ioy158

Cited by 12 publications

(12 citation statements)

References 81 publications

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“…Epidemiological studies have described that any defects during epigenetic reprogramming, including the imprinting process, may increase the risk of genetic and epigenetic disorders [ 44 , 45 ]. Mouse models have been used to study and observe embryonic abnormalities and offspring diseases [ 46 , 47 ], but the precise molecular mechanisms underlying these malformations remain unclear. It is a limit that congenital birth defects were based on telephone interviews; besides, the birth defects may need further genetic testing and phenotypic analysis.…”

Section: Discussionmentioning

confidence: 99%

Fertilization and neonatal outcomes after early rescue intracytoplasmic sperm injection: a retrospective analysis of 16,769 patients

Zeng

Yao

Zhang

et al. 2022

Arch Gynecol Obstet

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Purpose To evaluate the efficacy and safety of short-term insemination and early-rescue intracytoplasmic sperm injection (ICSI), an approach that rescued oocytes with unclear second polar body 6 h after initial insemination by ICSI (early R-ICSI) to avoid total or near-total fertilization failure in conventional in vitro fertilization (IVF). Methods We performed a retrospective study in 16,769 patients (short-term IVF, n = 12,094; ICSI, n = 3452; early R-ICSI, n = 1223) who received IVF/ICSI treatment in our hospital from January 2009 to October 2018. Fertilization and clinical outcomes were compared among those three groups. Results When considering the R-ICSI embryos in the early R-ICSI group independently, the rates of fertilization and day-3 cleaved embryos in 2PN oocytes were comparable, the rates of fertilization (2PN) and high-quality embryos were lower, whereas the multi-PN fertilization rate (3.27%) was significantly higher than the ICSI group (1.26%). The difference of clinical pregnancy rate between the part of transferred R-ICSI embryos (40.81%) and the ICSI group (44.73%) remained nonsignificant. Furthermore, the rate of congenital birth defects in the early R-ICSI group (0.99%) was not significantly different from those in the short-term IVF (0.76%) and ICSI groups (1.07%). Conclusion Despite the multi-PN fertilization rate, our study highlights early R-ICSI as a safe and effective alternative in assisted reproduction to decrease complete IVF fertilization failure and reduce ICSI utilization. Additional large amount and long-term follow-up studies are needed to further validate the use of early R-ICSI.

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Section: Discussionmentioning

confidence: 99%

Fertilization and neonatal outcomes after early rescue intracytoplasmic sperm injection: a retrospective analysis of 16,769 patients

Zeng

Yao

Zhang

et al. 2022

Arch Gynecol Obstet

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“…Other groups have found endothelial dysfunction and hypertension in IVF-produced mice offspring [ 44 , 45 ], which could be associated with risk of premature cardiovascular morbidity. Moreover, ART-produced female offspring showed altered expression of the renin-angiotensin system, which regulates blood pressure and is known to play a role in the pathogenesis of CVD [ 46 ]. Several experimental studies in mouse have demonstrated that ART can alter glucose metabolism, from simple hyperglycaemia to impaired glucose tolerance, which can lead to hyperinsulinemia and in some cases, to insulin resistance [ 36 , 47–49 ].…”

Section: Art and Metabolic Alterationsmentioning

confidence: 99%

“…It is evident that ART procedures affect metabolic health and disease in a sex-specific manner. Female ART-conceived offspring are more susceptible to cardiovascular alterations and obesity at a young age than their counterpart male offspring [ 42 , 46 ]. However, in male offspring, the alterations become more evident later in life, suggesting the possible risk of further disease development [ 36 , 42 ].…”

Section: Phenotypical Plasticitymentioning

confidence: 99%

The effects of assisted reproduction technologies on metabolic health and disease†

Heber

Ptak

2020

Biology of Reproduction

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The increasing prevalence of metabolic diseases places a substantial burden on human health throughout the world. It is believed that predisposition to metabolic disease starts early in life, a period of great susceptibility to epigenetic reprogramming due to environmental insults. Assisted reproductive technologies (ART), i.e., treatments for infertility, may affect embryo development, resulting in multiple adverse health outcomes in postnatal life. The most frequently observed alteration in ART pregnancies is impaired placental nutrient transfer. Moreover, consequent intrauterine growth restriction and low birth weight followed by catch-up growth can all predict future obesity, insulin resistance, and chronic metabolic diseases. In this review, we have focused on evidence of adverse metabolic alterations associated with ART, which can contribute to the development of chronic adult-onset diseases, such as metabolic syndrome, type 2 diabetes, and cardiovascular disease. Due to high phenotypic plasticity, ART pregnancies can produce both offspring with adverse health outcomes, as well as healthy individuals. We further discuss the sex-specific and age-dependent metabolic alterations reflected in ART offspring, and how the degree of interference of a given ART procedure (from mild to more severe manipulation of the egg) affects the occurrence and degree of offspring alterations. Over the last few years, studies have reported signs of cardiometabolic alterations in ART offspring that are detectable at a young age but that do not appear to constitute a high risk of disease and morbidity per se. These abnormal phenotypes could be early indicators of the development of chronic diseases, including metabolic syndrome, in adulthood. The early detection of metabolic alterations could contribute to preventing the onset of disease in adulthood. Such early interventions may counteract the risk factors and improve the long-term health of the individual.

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“…Recently, the effect of ART on the angiotensin II receptors (AT1R and AT2R) has gained attention. Wang and colleagues found increased cardiac AT1R mRNA expression in ART mice starting the third week after birth and persisting for as long as 1.5 years [ 9 ]. Moreover, Zhang and colleagues reported partial hypomethylation of the At1bR gene in the umbilical vein of human ART [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Increased Arterial Responsiveness to Angiotensin II in Mice Conceived by Assisted Reproductive Technologies

Meister

Soria

Dogar

et al. 2022

IJMS

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Since the first report in 1978, the number of individuals conceived by Assisted Reproductive Technologies (ART) has grown incessantly. In parallel, with the recent emergence of possible underlying mechanisms of ART-induced epigenetic changes in the renin-angiotensin system, the cardiovascular repercussions of ART in mice and human offspring (including arterial hypertension, vascular dysfunction, and cardiac remodeling) have become increasingly recognized. Here, we hypothesized that ART may increase arterial responsiveness to angiotensin II (ANG II) by epigenetically modifying the expression of its receptors. To test this hypothesis, we assessed the vasoconstrictor responsiveness to ANG II in isolated aortas from ART and control mice. We also examined ANG II receptor (ATR) type 1 and 2 expression and the promoter methylation of the At1aR, At1bR and At2R genes. We found that the vasoconstrictor response to ANG II was markedly increased in ART mice compared to controls. This exaggerated vasoconstrictor responsiveness in ART mice correlated with a significant increase in the ANG II receptor (ATR) type 1 to ATR type 2 protein expression ratio in the aorta; this was mainly driven by an increase in AT1R expression, and by hypomethylation of two CpG sites located in the At1bR gene promoter leading to increased transcription of the gene. We conclude that in mice, ART increase the vasoconstrictor response to ANG II in the aorta by epigenetically causing an imbalance between the expression of vasoconstrictor (AT1R) and vasodilator (AT2R) ANG II receptors. Unbalanced expression of AT1R and AT2R receptors seems to be a novel mechanism contributing to ART-induced arterial hypertension in mice.

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Alteration in the expression of the renin-angiotensin system in the myocardium of mice conceived by in vitro fertilization†

Cited by 12 publications

References 81 publications

Fertilization and neonatal outcomes after early rescue intracytoplasmic sperm injection: a retrospective analysis of 16,769 patients

Fertilization and neonatal outcomes after early rescue intracytoplasmic sperm injection: a retrospective analysis of 16,769 patients

The effects of assisted reproduction technologies on metabolic health and disease†

Increased Arterial Responsiveness to Angiotensin II in Mice Conceived by Assisted Reproductive Technologies

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