Alteration in polyamine titres during Agrobacterium-mediated transformation of indica rice with ornithine decarboxylase gene affects plant regeneration potential

Kumria, R.; Rajam, Manchikatla Venkat

doi:10.1016/s0168-9452(02)00020-1

Cited by 24 publications

(9 citation statements)

References 21 publications

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“…The regeneration capability of cultured cells seemed to be well maintained, and plants were quickly regenerated on media that contained maltose. A similar observation was reported for a protoplast-derived callus of indica rice IR43 (Gosh Biswas and Zapata, 1993) and for the transformation of cultivar Pusa Basmati 1 (Azhakanandam et al, 2000;Kumria et al, 2001;Kumria and Rajam, 2002).…”

Section: Discussionsupporting

confidence: 78%

“…Aldemita and Hodges (1996) succeeded in the Agrobacterium-mediated transformation of Group I varieties for the first time and the transformation frequency (independent transgenic plants per immature embryo infected) was 0.7% for TSC10 and 4.2% for IR72. Successful transformation of callus cultures mediated by Agrobacterium was also reported in Pusa Basmati I (Zhang et al, 1997;Mohanty et al, 1999;Azhakanandam et al, 2000;Kumria et al, 2001;Kumria and Rajam, 2002) and IR64 (Khanna and Raina, 2002). The transformation frequency (independent transgenic plants per callus piece infected) of IR64 was between 2.5 and 10.1% (Khanna and Raina, 2002).…”

Section: Introductionmentioning

confidence: 70%

“…Bars in the photographs represent 1 mm. (Zhang et al, 1997;Mohanty et al, 1999;Azhakanandam et al, 2000;Kumria et al, 2001;Kumria and Rajam, 2002;Khanna and Raina, 2002) and IR64 Raina, 1999, 2002). For the transformation of calluses, the use of embryogenic calluses that consist of actively dividing cells is very important (Hiei et al, 1994;Dong et al, 1996;Rashid et al, 1996).…”

Section: Discussionmentioning

confidence: 98%

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Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens

Hiei

Komari

2006

Plant Cell Tiss Organ Cult

101

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A highly efficient gene transfer method mediated by Agrobacterium tumefaciens was developed for Group I indica rice, which had been quite recalcitrant in tissue culture and transformation. Freshly isolated immature embryos from plants grown in a greenhouse were inoculated with A. tumefaciens LBA4404 that harbored super-binary vector pTOK233 or pSB134, which had a hygromycin-resistance gene and a GUS gene in the T-DNA. The efficiency of gene transfer varied with the kinds of gelling agents and the basic compositions of co-cultivation media. The highest activity of GUS after co-cultivation was observed when NB medium solidified with agarose was used. For the subsequent cultures, two types of media (modified NB and CC) were chosen to recover hygromycin-resistant cells efficiently. The transformation protocol thus developed worked very well in all of the varieties tested in this study, and the transformation frequency (number of independent hygromycin-resistant and GUS-positive plants per embryo) reached more than 30% in IR8, IR24, IR26, IR36, IR54, IR64, IR72, Xin Qing Ai 1, Nan Jin 11, and Suewon 258. Most of the transformants (T 0 ) were normal in morphology and fertile. Stable integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis of transformants in the T 0 and T 1 generations. For the recovery of multiple independent transgenic events from a single immature embryo, procedures were developed to section the embryo into as many as 30 pieces after non-selective cultures following co-cultivation. Transformants were then obtained from the pieces cultured on the selective media, and, in the highest case, more than seven independent transgenic plants per original embryo (transformation frequency of 738%) were produced. Thus, the efficiency of transformation was remarkably improved.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 98%

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Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens

Hiei

Komari

2006

Plant Cell Tiss Organ Cult

101

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“…Previous reports have also found maltose to be a better carbon source for regeneration [52][53][54]. According to Lentini et al [55], maltose has been described as an agent that may regulate osmotic potential of cellular environment of callus.…”

Section: Resultsmentioning

confidence: 99%

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

Sah¹,

Kaur²

2014

J Rice Res

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A new and rapid protocol for production of embryogenic callus and plant regeneration has been reported in Oryza sativa cv. Kitaake. Callus cultures were established from seeds of Kitaake on MS medium supplemented with 2,4-D (3.0 mg/l), BAP (0.25 mg/l) and proline (0.6 g/l) gelled with phytagel (3.0 g/l), after 9 days of seed culturing. The embryogenic calli were regenerated on regeneration medium, i.e. MS medium supplemented with BAP (3.0 mg/l) and NAA (0.2 mg/l) and gelled with agar (8.0 g/l) in combination with phytagel (2.0 g/l) and regeneration occurred in 18 days. The regeneration percentage achieved was very high i.e 82.66% on the above medium. Rooting was achieved on half strength MS medium. The plantlets were hardened and transferred to soil in earthen pots. The regeneration protocol so developed through callus formation was highly reproducible. The plants showed normal growth and flowering under glass house conditions as well as in field conditions.

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“…Increased salt tolerance has been observed in tobacco plants that accumulate putrescine by constitutive over-expression of mouse ODC (Kumria and Rajam 2002). In rice, tolerance to drought is improved by constitutive over-expression of oat ADC (Capell et al 1998).…”

Section: Gain-of-function and The Stress Responsementioning

confidence: 99%

Involvement of polyamines in plant response to abiotic stress

et al. 2006

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Environmental stresses are the major cause of crop loss worldwide. Polyamines are involved in plant stress responses. However, the precise role(s) of polyamine metabolism in these processes remain ill-defined. Transgenic approaches demonstrate that polyamines play essential roles in stress tolerance and open up the possibility to exploit this strategy to improve plant tolerance to multiple environmental stresses. The use of Arabidopsis as a model plant enables us to carry out global expression studies of the polyamine metabolic genes under different stress conditions, as well as genome-wide expression analyses of insertional-mutants and plants over-expressing these genes. These studies are essential to dissect the polyamine mechanism of action in order to design new strategies to increase plant survival in adverse environments.

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Alteration in polyamine titres during Agrobacterium-mediated transformation of indica rice with ornithine decarboxylase gene affects plant regeneration potential

Cited by 24 publications

References 21 publications

Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens

Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

Involvement of polyamines in plant response to abiotic stress

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