Involvement of polyamines in plant response to abiotic stress

Alcázar, Rubén; Marco, Francisco; Cuevas, Juan C.; Patron, Macarena; Ferrando, Alejandro; Carrasco, Pedro; Tiburcio, Antonio F.; Altabella, Teresa

doi:10.1007/s10529-006-9179-3

Cited by 524 publications

(374 citation statements)

References 54 publications

(50 reference statements)

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“…PAs have also been described as membrane protectors (Alcázar et al, 2006;Kusano et al, 2008), a property that is consistent with the observation that the addition of spermine dramatically inhibited H 2 O 2 -induced ion leakage in WT plants (Fig. 10B).…”

Section: Discussionsupporting

confidence: 87%

“…7A and 7B). In plants, PAs are present as free bases or conjugated to small molecules, mainly hydroxycinnamic acids, and to various macromolecules such as proteins (Alcázar et al, 2006). It was recently suggested that there is a reverse PA pathway in plants from spermine to putrescine (Kusano et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…In plants, the major PAs putrescine, spermidine, and spermine have been shown to be involved in development, senescence and stress responses (Alcázar et al, 2006). Cadaverine, which is relatively less abundant, is present in various bacteria and in several higher plant families such as Gramineae, Poaceae, Solanaceae, and in particular, Leguminosae (Ohe et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Increased Polyamine Biosynthesis Enhances Stress Tolerance by Preventing the Accumulation of Reactive Oxygen Species: T-DNA Mutational Analysis of Oryza sativa Lysine Decarboxylase-like Protein 1

Jang

Choi

et al. 2012

Molecules and Cells

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A highly oxidative stress-tolerant japonica rice line was isolated by T-DNA insertion mutation followed by screening in the presence of 50 mM H 2 O 2 . The T-DNA insertion was mapped to locus Os09g0547500, the gene product of which was annotated as lysine decarboxylase-like protein (GenBank accession No. AK062595). We termed this gene OsLDC-like 1, for Oryza sativa lysine decarboxylase-like 1. The insertion site was in the second exon and resulted in a 27 amino acid N-terminal deletion. Despite this defect in OsLDC-like 1, the mutant line exhibited enhanced accumulation of the polyamines (PAs) putrescine, spermidine, and spermine under conditions of oxidative stress. The generation of reactive oxygen species (ROS) in the mutant line was assessed by qRT-PCR analysis of NADPH oxidase (RbohD and RbohF), and by DCFH-DA staining. Cellular levels of ROS in osldc-like 1 leaves were significantly lower than those in the wild-type (WT) rice after exposure to oxidative, high salt and acid stresses. Exogenouslyapplied PAs such as spermidine and spermine significantly inhibited the stress-induced accumulation of ROS and cell damage in WT leaves. Additionally, the activities of ROS-detoxifying enzymes were increased in the homozygous mutant line in the presence or absence of H 2 O 2 . Thus, mutation of OsLDC-like 1 conferred an oxidative stress-tolerant phenotype. These results suggest that increased cellular PA levels have a physiological role in preventing stress-induced ROS and ethylene accumulation and the resultant cell damage.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Increased Polyamine Biosynthesis Enhances Stress Tolerance by Preventing the Accumulation of Reactive Oxygen Species: T-DNA Mutational Analysis of Oryza sativa Lysine Decarboxylase-like Protein 1

Jang

Choi

et al. 2012

Molecules and Cells

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“…The accumulation of amino acids, especially Pro and Arg, contributes to the osmoprotection of plants (Rai, 2002). Increase in Arg content may lead to the accumulation of polyamines which protect as polycations the vital negatively charged, macromolecules in the cells (Alcázar et al, 2006). In addition, reactive oxygen species, antioxidants, amino acids and polyamines are all involved in signalling pathways which may activate further protective mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide affects salt-induced changes in free amino acid levels in maize

Boldizsár¹,

Simon‐Sarkadi²,

Szirtes³

et al. 2013

Journal of Plant Physiology

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Abstract:It was assumed that salt-induced redox changes affect amino acid metabolism in maize (Zea mays L.), and this influence may be modified by NO. The applied NaCl treatment reduced the fresh weight of shoots and roots. This decrease was smaller after the combined application of NaCl and an NO-donorETA/NO) in the shoots, while it was greater after simultaneous treatment with NaCl and nitro-l-arginine (l-NNA, inhibitor of NO synthesis) in the roots. The quantum yield efficiency of photosystem II was not influenced by the treatments. NaCl had a significant effect on the redox environment in the leaves as it was shown by the increase in the amount of glutathione disulphide and in the redox potential of the glutathione/glutathione disulphide redox pair. This influence of NaCl was modified by DETA/NO and l-NNA. Pharmacological modification of NO levels affected salt-induced changes in both the total free amino acid content and in the free amino acid composition. NaCl alone increased the concentration of almost all amino acids which effect was strengthened by DETA/NO in the case of Pro. l-NNA treatment resulted in a significant increase in the Ala, Val, Gly and Tyr contents. The Ile, Lys and Val concentrations rose considerably after the combined application of NaCl and DETA/NO compared to NaCl treatment alone in the recovery phase. NaCl also increased the expression of several genes related to the amino acid and antioxidant metabolism, and this effect was modified by DETA/NO. In conclusion, modification of NO levels affected salt-induced, glutathione-dependent redox changes and simultaneously the free amino acid composition and the level of several free amino acids. The observed much higher Pro content in plants treated with both NaCl and DETA/NO during recovery may contribute to the protective effect of NO against salt stress.

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“…1 They have been implicated in a wide range of biological processes, including cell growth and division, stabilization of nucleic acids and membranes, protein synthesis and chromatin function, and biotic and abiotic stresses responses. [2][3][4][5] The most common PAs are spermidine and spermine, together with their diamine precursor putrescine. The biosynthesis of PAs is controlled in plants primarily by three enzymes: arginine decarboxylase (ADC; EC 4.1.…”

Section: Introductionmentioning

confidence: 99%