Alteraciones fisiológicas asociadas a la infección con Mal de Río Cuarto virus (MRCV) y a fitotoxicidad provocada por su insecto vector (Delphacodes kuscheli Fennah) en trigo

Abstract

RESUMENMal de Río Cuarto virus en trigo provoca una enfermedad resultante de la acción combinada con su principal vector, Delphacodes kuscheli. La severa sintomatología inducida por virus e insecto condujo a determinar la posible existencia de alteraciones fisiológicas en inducida por virus e insecto condujo a determinar la posible existencia de alteraciones fisiológicas en el cultivar de trigo ProINTA Federal, infectado artificialmente con dos aislamientos geográficamente distantes del MRCV y sometido a picadura de insectos no virulíferos, respectivamente, en tres estadios fenológicos. ProINTA Federal fue picado por insectos no virulíferos e inoculado en la primera hoja emergiendo del coleóptilo, en la cuarta y quinta hojas desplegadas, respectivamente. La condición sanitaria de los diferentes tratamientos fue constatada mediante ELISA. Las plantas tratadas manifestaron alteraciones fisiológicas con similares variaciones entre estadios fenológicos. Azúcares solubles totales, almidón y proteínas solubles incrementaron significativamente su contenido en plantas infectadas por MRCV. En plantas PS y con MRCV, el contenido de clorofilas disminuyó marcadamente y lo inverso sucedió con el malondialdehído, lo que indicaría daño oxidativo por estrés en las mismas. No existieron diferencias entre estadios fenológicos y las variaciones de los indicadores de alteraciones fisiológicas considerados, excepto para el incremento de almidón, significativamente superior en plantas inoculadas en la primera hoja emergiendo del coleóptilo. Palabras clave: infección viral, toxicidad por insectos, azúcares solubles, almidón, proteínas, clorofilas, malondialdehído, estadios fenológicos. ABSTRACT Physiological alterations associated to the Mal de Rio Cuarto virus (MRCV) infection and to vector (Delphacodes kuscheli Fennah) phytotoxicity in wheatMal de Río Cuarto virus produces a wheat disease, resulting from the combined action of this pathogen and its main vector, resulting from the combined action of this pathogen and its main vector, from the combined action of this pathogen and its main vector, of this pathogen and its main vector, this pathogen and its main vector, Delphacodes kuscheli. Knowing that viruses and insects cause a severe symptomatology in different genotypes, the objective of this nowing that viruses and insects cause a severe symptomatology in different genotypes, the objective of this severe symptomatology in different genotypes, the objective of this in different genotypes, the objective of this genotypes, the objective of this the objective of this the objective of this of this work was to determine the possible physiological alterations in three phenological stages of wheat (cv ProINTA Federal) inoculated by was to determine the possible physiological alterations in three phenological stages of wheat (cv ProINTA Federal) inoculated by possible physiological alterations in three phenological stages of wheat (cv ProINTA Federal) inoculated by le physiological alterations in three phenological stages of wheat (cv Pro...

Attenuation of Zucchini mosaic virus disease in cucumber plants by mycorrhizal symbiosis

“…The amino acid proline functions as a powerful ROS scavenger, redox balancer, molecular chaperon, and protein structural stabilizer, which may inhibit the triggering of programmed cell death by ROS (Chen and Dickman 2005 ; Soni et al 2022 ). Our results of protein accumulation under ZYMV infection were in line with those of Di Feo et al ( 2010 ) due to the biotic stress of MRCV in wheat plants. Similarly, in response to ZYMV infection, Radwan et al ( 2007 ) found a considerable rise in the amounts of soluble, insoluble, and total proteins in C. pepo leaves, and they also noted that the newly synthesized polypeptides that appeared in the ZYMV-infected sample are believed to be pathogenesis-related proteins.…”

“…6 a). These results are in line with earlier studies, which found that MDA levels were greater in ZYMV- and Mal de Rio Cuarto virus (MRCV)-infected pumpkin and wheat plants, respectively (Radwan et al 2007 ; Di Feo et al 2010 ). Siddique et al ( 2014 ) investigated how another viral disease, leaf curl disease caused by CLCuV, affected the leaves of two susceptible and resistant cotton genotypes, and found that after infection, the susceptible genotype leaves had higher MDA concentrations.…”

Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves

“…Sucrose accumulation in leaves has been repeatedly reported after virus infections [ 83 , 84 ]. Soluble sugars and starch accumulate in MRCV-infected wheat leaves during late stages of infection [ 85 ], while several proteins involved in carbohydrate metabolism and photosynthesis were differentially accumulated in maize leaf tumours caused by RBSDV [ 86 ]. However, to the best of our knowledge, Tre6P accumulation has never been reported in virus-infected plants.…”

Insights Into Insect Vector Transmission and Epidemiology of Plant-Infecting Fijiviruses

“…The physiology is altered in MRCV-infected wheat plants; the contents of total soluble sugar, starch, protein and malondialdehyde levels increase noticeably, but chlorophyll content decreases considerably. These variations are indicative of oxidative damage associated with biotic stress in these plants (Di Feo et al, 2010). MRCV-infected wheat plants had more than 3,000 differentially accumulated transcripts (DATs) at 21 days post inoculation, and exhibited higher levels of soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids, but decreased transcripts levels for TaSWEET13, which are involved in sucrose phloem loading (de Haro et al, 2019).…”