alpha-Interferon potentiates the growth inhibitory effects of anti-transferrin receptor monoclonal antibodies

Caraglia, Michele; Leardi, A.; Ricciardi, B; DeLaurentiis, Michelino; Graziano, Maria Gabriella; Deplacido, S; Bianco, Angelo Raffaele; Tagliaferri, Pierosandro

doi:10.3892/ijo.8.5.847

Cited by 2 publications

(2 citation statements)

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“…In fact, we have described an increased expression of functional TRF-R on human epidermoid carcinoma KB cells following exposure to cytostatic concentrations of IFNa. We have found in these cells a 3-fold reduction of intracellular iron storages which were restored to normal when TRF-R expression was upregulated (Caraglia et al, , 1996.…”

Section: Discussionmentioning

confidence: 75%

Desferioxamine increases iron depletion and apoptosis induced by ara‐C of human myeloid leukaemic cells

et al. 1998

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Summary.We investigated whether changes in iron metabolism and the transferrin receptor (TRF-R) expression were involved in the antileukaemic effects of arabinoside cytosine (ara-C). Treatment with 100 nM ara-C for 48 h reduced thymidine uptake and increased the surface expression of the TRF-R on leukaemic blasts derived from 13/16 (81%) patients and on the HL-60 and U-937 cell lines. Whereas intracellular non-haem iron was strongly depleted 24 h after ara-C addition, TRF-R up-regulation and recovery of intracellular non-haem iron concentration occurred together after a longer exposure of the cultured cells to the drug. Since iron is an essential regulator of cell proliferation we have evaluated the effects of the combination between ara-C and the iron chelator desferioxamine (DSF) on the growth of HL-60 and U-937 cells. We found that desferioxamine strongly potentiated the effects of ara-C on leukaemic cell growth inhibition and apoptosis. This is the first report of a positive interaction between ara-C and an iron chelator in terms of antileukaemic effects.

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Section: Discussionmentioning

confidence: 75%

Desferioxamine increases iron depletion and apoptosis induced by ara‐C of human myeloid leukaemic cells

et al. 1998

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“…In this light we have recently reported that INF‐α upregulates the cell receptor for EGF and transferin (TRF) in human epidermoid cancer cells [96,208–210]. EGF‐R expression is also increased by other antiproliferative agents such as retinoic acid, tumour necrosis factor‐α, conventional anticancer drugs at a low concentration, cytosine arabinoside and 5‐aza‐2′deoxycytidine [211–220]. Although these studies showed that the binding of immunotoxins to target cells could be increased, they showed no inhibition of the onset of protective responses involving signalling and protein translation that can overcome the antiproliferative activity of the toxin.…”

Section: Increase In Cytotoxic Effects Of Agents Raised Against Protementioning

confidence: 99%

Modulation of molecular mechanisms involved in protein synthesis machinery as a new tool for the control of cell proliferation

Caraglia¹,

Budillon

Vitale

et al. 2000

European Journal of Biochemistry

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In the past years, the attention of scientists has focused mainly on the study of the genetic information and alterations that regulate eukaryotic cell proliferation and that lead to neoplastic transformation. All therapeutic strategies against cancer are, to date, directed at DNA either with cytotoxic drugs or gene therapy. Little or no interest has been aroused by protein synthesis mechanisms. However, an increasing body of data is emerging about the involvement of translational processes and factors in control of cell proliferation, indicating that protein synthesis can be an additional target for anticancer strategies. In this paper we review the novel insights on the biochemical and molecular events leading to protein biosynthesis and we describe their involvement in cell proliferation and tumorigenesis. A possible mechanistic explanation is given by the interactions that occur between protein synthesis machinery and the proliferative signal transduction pathways and that are therefore suitable targets for indirect modulation of protein synthesis. We briefly describe the molecular tools used to block protein synthesis and the attempts made at increasing their efficacy. Finally, we propose a new multimodal strategy against cancer based on the simultaneous intervention on protein synthesis and signal transduction.

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alpha-Interferon potentiates the growth inhibitory effects of anti-transferrin receptor monoclonal antibodies

Cited by 2 publications

References 0 publications

Desferioxamine increases iron depletion and apoptosis induced by ara‐C of human myeloid leukaemic cells

Desferioxamine increases iron depletion and apoptosis induced by ara‐C of human myeloid leukaemic cells

Modulation of molecular mechanisms involved in protein synthesis machinery as a new tool for the control of cell proliferation

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