Desferioxamine increases iron depletion and apoptosis induced by ara‐C of human myeloid leukaemic cells

Leardi, A.; Caraglia, Michele; Selleri, Carmine; Pepe, Stefano; Pizzi, C.; Notaro, Rosario; Fabbrocini, Antonietta; Lorenzo, Sonya De; Musico, M.; Abbruzzese, Alberto; Bianco, Angelo Raffaele; Tagliaferri, Pierosandro

doi:10.1046/j.1365-2141.1998.00834.x

Cited by 103 publications

(55 citation statements)

References 23 publications

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“…Erythrocytes and plasma free iron levels were determined according to Leardi et al (1998) using a commercially available kit from Biomaghreb. Briefly, at acidic pH 4.8 all Fe 3+ released from transferrin were reduced by ascorbic acid into Fe 2+ , which constitutes with ferrozine a purple colourful complex measurable at 560 nm.…”

Section: Free Iron Determinationmentioning

confidence: 99%

Grape seed and skin extract protects against acute chemotherapy toxicity induced by doxorubicin in rat red blood cells and plasma

Hamlaoui

Mokni

Limam

et al. 2012

Bangladesh J Pharmacol

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In this study, the protective role of grape seed and skin extract (GSSE) against doxorubicin-induced blood toxicity has been evaluated in rats. Rats were treated with the extract for 8 days and injected with doxorubicin (20 mg/kg) at the 4th day. At the end of the treatment, blood samples were collected for oxidative stress parameters determination and anti-oxidant enzymes. Doxorubicin increased erythrocytes and plasma malondialdehyde, free iron, H2O2 and carbonylation, decreased calcium and also decreased erythrocytes catalase, peroxidase and superoxide dismutase (specially the Fe isoform). Doxorubicin also decreased plasma catalase and superoxide dismutase (Cu/ Zn and Fe isoforms) but increased peroxidase. Doxorubicin increased plasma alanine aminotransferase and aspartate aminotransferase but decreased them within erythrocytes. GSSE co-treatment counteracted almost all deleterious effects induced by doxorubicin. In conclusion, doxorubicin induced a prooxidative stress into rat erythrocytes and plasma and GSSE exerted antioxidant properties which can be attributed to free iron and calcium modulation. Article Info

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Section: Free Iron Determinationmentioning

confidence: 99%

Grape seed and skin extract protects against acute chemotherapy toxicity induced by doxorubicin in rat red blood cells and plasma

Hamlaoui

Mokni

Limam

et al. 2012

Bangladesh J Pharmacol

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“…Although it has been shown that DFB has a toxic effect on eukaryotic cells (Fukuchi et al 1994, Leardi et al 1998, we have demonstrated that this is not the case for our heterotrophic bioreporter (Fig. 5).…”

Section: Specificity Of Fe-siderophore Acquisition and Cross-utilizationmentioning

confidence: 78%

Virus and siderophore-mediated transfer of available Fe between heterotrophic bacteria: characterization using an Fe-specific bioreporter

Mioni¹,

Poorvin²,

Wilhelm³

2005

Aquat. Microb. Ecol.

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Although marine chemists can accurately quantify both the concentration of dissolved iron (Fe) and the high-affinity organic ligands which complex Fe in surface waters, tools to characterize the relative bioavailability of such organically bound Fe complexes remain unavailable. In this study, we compared the bioavailability of Fe released from the lysis of the heterotrophic bacterium Vibrio natriegens PWH3a to that of Fe complexed to synthetic chelators (EDTA) and siderophores (including the trihydroxamate desferrioxamine B [DFB] and 2 catecholates isolated from Fe-limited heterotrophic bacterial cultures) using a heterotrophic bioluminescent reporter of Fe availability (Pseudomonas putida FeLux). Using the bioluminescent response of P. putida FeLux, we were able to rank the Fe sources tested here in a decreasing order of bioavailability: lysates > Fe-homologous catecholate (from a P. putida FeLux culture) ~ Fe-exogenous catecholate (from V. natriegens culture) > inorganic Fe (FeCl 3 , 15 nM) ~ Fe(III)' from EDTA-buffered treatment (pFe 18.12) > Fe:DFB, where pFe is -log [Fe 3+ ]. Combined with estimates of Fe assimilation of 55 Fe-labeled lysates, our data further demonstrate that organic Fe complexes released during virus-mediated cell lysis are ca. 1000 times more bioavailable and efficiently assimilated by bacterial cells than Fe(III)'. Our results validate the utilization of P. putida FeLux as a bioreporter of Fe-bioavailability and also support the assumption that virus activity plays a crucial role in the regeneration of biologically available Fe complexes in surface seawater. KEY WORDS: Iron availability · Bioreporter · Heterotrophic bacteria · Siderophore · Virus lysates Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 41: [233][234][235][236][237][238][239][240][241][242][243][244][245] 2005 these organic ligands in natural systems, recent experiments suggest that the chemical nature of these Fe complexes may influence competition between prokaryotes and eukaryotes (Hutchins et al. 1999a, Poorvin et al. 2004.The similarities between the stability constants ( Lewis et al. 1995, Rue & Bruland 1995 and structural groups (Martinez et al. 2003, Gledhill et al. 2004) of the natural organic Fe ligands and those for naturally produced siderophores has led to a surge of interest in the microbial component of the marine plankton assemblage. It has been demonstrated that marine heterotrophic bacteria account for as much as 50% of the biomass (and thus biogenic Fe) in oceanic systems (Tortell et al. 1999). Furthermore, bacteria contain significantly more Fe per biomass unit than phytoplankton and are responsible for significant Fe uptake in Fe-depleted seawaters (Tortell et al. 1996). In addition, although several studies have shown that bacterial productivity was co-limited by carbon and Fe (Church et al. 2000, Kirchman et al. 2000, other studies suggest that heterotrophic bacterial productivity may be directly Fe-limited at low, in situ concentra...

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“…The interaction of TNK2 in vivo with clathrin and AP-2 participated in various trafficking pathways, underlying an ability to increase receptor-mediated transferrin uptake (37,39). It is well known that iron is essential for cell proliferation, and the iron was suggested to enhance the cell proliferation or anti-apoptotic effect (40)(41)(42)(43). The up-regulation of TNK2 and AP2B1 might induce the iron uptake into the leukemic cells, and the iron might play an important role for re-growth of the leukemic cells.…”

Section: Discussionmentioning

confidence: 99%

Prediction of risk of disease recurrence by genome-wide cDNA microarray analysis in patients with Philadelphia chromosome-positive acute lymphoblastic leukemia treated with imatinib-combined chemotherapy

Zembutsu

Yanada²,

Hishida³

et al. 2007

Int J Oncol

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Abstract. Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ALL) reveals very poor prognosis due to high incidence of relapse when treated with standard chemotherapy. Although >96% of patients with Ph+ALL achieved complete remission (CR) with imatinib-combined chemotherapy in a phase II clinical trial conducted by the Japan Adult Leukemia Study Group (JALSG), 26% of them experienced hematological relapse in a short time after achievement of CR. In this study, to establish a prediction system for risk of relapse, we analyzed gene expression profiles of 23 bone marrow samples from patients with Ph+ALL using cDNA microarray consisting of 27,648 cDNA sequences. Using the 19 randomly-selected test cases, we identified 16 genes that were expressed significantly differently between patients with (n=8) and without (n=11) continuous response; from the list of 16 genes, we selected the 6 'predictive' genes and constructed a numerical scoring system by which the two groups were clearly separated, with positive scores for the former and the negative scores for the latter. Scoring of 4 cases that were reserved from the original 23 cases predicted correctly their clinical responses. In addition, three cases whose BCR-Abl transcript levels failed to reduce sufficiently after induction therapy, also revealed negative scores. We also developed a quantitative reverse transcription-PCR-based prediction system that could be feasible for routine clinical use. Our results suggest that achievement of continuous response with imatinib-combined chemotherapy can be predicted by expression patterns in this set of genes, leading to achievement of 'personalized therapy' for treatment of this disease.

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Desferioxamine increases iron depletion and apoptosis induced by ara‐C of human myeloid leukaemic cells

Cited by 103 publications

References 23 publications

Grape seed and skin extract protects against acute chemotherapy toxicity induced by doxorubicin in rat red blood cells and plasma

Grape seed and skin extract protects against acute chemotherapy toxicity induced by doxorubicin in rat red blood cells and plasma

Virus and siderophore-mediated transfer of available Fe between heterotrophic bacteria: characterization using an Fe-specific bioreporter

Prediction of risk of disease recurrence by genome-wide cDNA microarray analysis in patients with Philadelphia chromosome-positive acute lymphoblastic leukemia treated with imatinib-combined chemotherapy

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