Alpha-fetoprotein in bovine hepatocellular carcinoma

Kithier, Karel; Al‐Sarraf, Muhyi; Belamaric, J.; Rádl, J.; Valenta, Z.; Žižkovský, Václav; Masopust, J

doi:10.1016/0021-9975(74)90036-x

Cited by 10 publications

(6 citation statements)

References 17 publications

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“…9,11 AFP and CK18 are also known as tumor markers of HCC. 3,4,8 Currently, it has been reported and accepted that myofibroblasts play an important role in hepatic fibrosis for the production of collagen fibers resulting in fibrous septa or capsules. Specifically, myofibroblasts can express ␣-SMA, and ␣-SMA-positive spindle cells were detected in the areas of fibrosis, within the neoplastic regions.…”

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confidence: 99%

“…6 There are relatively few reports on bovine HCC. 3,5 Incidence data and age distribution of HCC in cattle is not known because most cases were obtained at the time of slaughter.This report represents the gross, histopathologic, and immunohistochemical findings of a bovine HCC with metastasis to the spleen, found in a Holstein cow during a routine postmortem inspection at an abattoir. The cow was of normal appearance at the time of slaughter, without any preexisting medical conditions.…”

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confidence: 99%

“…6 There are relatively few reports on bovine HCC. 3,5 Incidence data and age distribution of HCC in cattle is not known because most cases were obtained at the time of slaughter.…”

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confidence: 99%

“…Some reports showed that there was a marked reduction in the number of Kupffer cells in poorly or moderately differentiated HCC. 3,10 We used anti-CD68 antibody to delineate Kupffer cells in bovine liver tissue. 1 Our study revealed a marked decrease of Kupffer cells within the HCC, when compared with their distribution in adjacent nonneoplastic liver tissue.…”

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Hepatocellular Carcinoma with Metastasis to the Spleen in a Holstein Cow

Jeong¹,

Do²,

Sohn³

et al. 2005

Vet Pathol

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Abstract. Hepatocellular carcinoma (HCC) with metastasis to the spleen in a Holstein cow was studied by histopathologic and immunohistochemical methods. The tumor was characterized by a pseudoglandular (acinar) pattern with an associated fibrous stroma. Individual cells often had a ''hepatoid'' appearance but were interspersed with scattered cells exhibiting a clear, periodic acid-Schiff (PAS)-positive cytoplasm and small eccentric nuclei. This pattern was present in nodules found in both liver and spleen. Moreover, hepatoid tumor cells were positive for alpha-fetoprotein. Immunohistochemical studies suggest that myofibroblasts were responsible for the production of fibrous septa surrounding the pseudoglandular structures of bovine HCC. In summary, our histologic and immunohistochemical findings support a diagnosis of primary HCC with splenic metastasis. Furthermore, the associated stromal response appears to be of a myofibroblast origin. The primary etiology of bovine HCC and the significance of the intralesional, PAS-positive clear cells remain undetermined.Key words: Alpha-fetoprotein; cows; hepatocellular carcinoma; myofibroblast.Hepatocellular carcinoma (HCC) has been reported in various species of animals including dogs, cats, sheep, pigs, fowl, woodchucks, and trout. 6 There are relatively few reports on bovine HCC. 3,5 Incidence data and age distribution of HCC in cattle is not known because most cases were obtained at the time of slaughter.This report represents the gross, histopathologic, and immunohistochemical findings of a bovine HCC with metastasis to the spleen, found in a Holstein cow during a routine postmortem inspection at an abattoir. The cow was of normal appearance at the time of slaughter, without any preexisting medical conditions. We also describe the myofibroblasts' involvement in the production of a fibrous stroma of bovine HCC.A 4-year-old Holstein cow was slaughtered. Postmortem inspection of the carcass revealed small white-gray nodules, ranging in size from several millimeters to 1 cm in diameter, randomly scattered on the liver surface. Also, there was peritonitis. However, the lungs appeared grossly normal. The nodules were nonencapsulated and poorly demarcated from the surrounding liver tissue. The spleen was enlarged, with hemorrhagic lymphoid follicles. The cut surface of the spleen revealed hemorrhaging and bulging lymphoid follicles, 0.3-0.5 mm in diameter.Representative sections of the liver and spleen were fixed immediately in 10% neutral-buffered formalin, processed routinely, and embedded in paraffin. Tissue sections were cut to 4 m thickness and stained with hematoxylin and eosin, periodic acid-Schiff (PAS), and Azan stain. For immunohistochemical studies, the primary antibodies used were: anti-␣-smooth muscle actin (␣-SMA) at a dilution of 1 : 800 (clone 1A4, Sigma Chemical Co., St. Louis, MO), anti-CD68 (EBM11) at a dilution of 1 : 80, alpha-fetoprotein (AFP) at a dilution of 1 : 800 (Dako, Carpenteria, CA), and anti-cytokeratin 18 (CK18) at a dilution of 1 : 100 (Nov...

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confidence: 99%

mentioning

confidence: 99%

“…6 There are relatively few reports on bovine HCC. 3,5 Incidence data and age distribution of HCC in cattle is not known because most cases were obtained at the time of slaughter.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Hepatocellular Carcinoma with Metastasis to the Spleen in a Holstein Cow

Jeong¹,

Do²,

Sohn³

et al. 2005

Vet Pathol

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“…27 One report of AFP detected in the serum of 2 of 4 bovids with hepatocellular carcinoma was found. 28 Those investigators used rabbit-derived antibody to fetal bovine serum and immunoelectrophoresis to measure AFP concentration. 28 We evaluated other serum biochemical variables for their usefulness in differentiating hepatic tumor from nonneoplastic hepatic disease.…”

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confidence: 99%

Detection of serum α-fetoprotein in dogs with hepatic tumors

Lowseth,

Gillett,

Chang

et al. 1991

javma

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Summary Serum α-fetoprotein (afp) concentration was detected by use of 2 commercially available kits containing antibodies to human afp—a radioimmunoassay and an enzymetric test. Using neonatal canine serum (a source high in afp), it was determined that reagents from both kits were able to bind to canine afp, but a significant difference was detected in afp concentration. The enzymetric test was superior in detecting canine afp. Sera from dogs were classified into 6 groups: from dogs with primary hepatic tumors only (group 1); from dogs with primary hepatic tumors and other tumors (group 2); from dogs with normal liver but with other types of neoplasia (group 3); from dogs with nonneoplastic hepatic disease and tumors originating in other organs (group 4); from dogs with nonneoplastic hepatic disease only (group 5); and from clinically normal dogs (group 6). Serum biochemical determinations (alkaline phosphatase, alanine transaminase, albumin, total protein, total bilirubin, and serum bile acids) and values from the 2 afp assays were obtained for all dogs. Serum afp concentration detected by the enzymetric test was significantly higher in dogs with hepatocellular carcinoma and cholangiocarcinoma. Values > 250 ng/ml were detected in 5 of 9 dogs with cholangiocarcinoma and in 3 of 4 dogs with hepatocellular carcinoma. High serum afp concentration also was indicative of liver involvement in 2 of 3 dogs with primary hepatic lymphosarcoma; 2 dogs had values > 225 ng/ml. Serum afp concentration in dogs with other types of hepatic tumors was < 250 ng/ml, and serum afp concentration could not be correlated with such tumors. Discriminatory analysis was done to determine which serum biochemical values best aided differentiation of hepatic neoplasia from nonneoplastic hepatic disease. Along with high serum afp concentration, high serum alkaline phosphatase activity and low albumin concentration were the most important analytes. If hepatic tumor is suspected, we recommend use of the enzymetric test to determine afp concentration, in addition to use of serum biochemical values, clinical findings, and radiography, to aid in the diagnosis.

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Tumors of the Liver and Gall Bladder

Cullen¹,

Popp²

2002

Tumors in Domestic Animals

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Alpha-fetoprotein in bovine hepatocellular carcinoma

Cited by 10 publications

References 17 publications

Hepatocellular Carcinoma with Metastasis to the Spleen in a Holstein Cow

Hepatocellular Carcinoma with Metastasis to the Spleen in a Holstein Cow

Detection of serum α-fetoprotein in dogs with hepatic tumors

Tumors of the Liver and Gall Bladder

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