Alpha‐amylase as molecular target for treatment of diabetes mellitus: A comprehensive review

Colvillea racemosa is a cultivated ornamental plant that is a monotypic genus of Fabaceae. It is native to Madagascar, with limited studies. For the first time, the leaf quality control parameters, the anti-hyperglycemic and anti-inflammatory in vitro activity of Colvillea racemosa ethanol extract (CRE) and its fractions of petroleum ether (CRP), methylene chloride (CRMC), ethyl acetate (CREA), n-butanol (CRB), and methanol (CRME) were evaluated. It exhibited significant inhibition against α-amylase, α-glucosidase and membrane stabilization. CRB was the most active fraction, and in vivo studies revealed that oral treatment with CRB of STZ-induced diabetic rats efficiently lowered blood glucose by 67.78%, reduced serum nitric oxide and lipid peroxide levels by 41.23% and 38.45%, respectively, and increased the GSH level by 90.48%. In addition, compared with the diabetic group, there was a 52.2% decrease in serum VCAM, a 55.5% increase in paraoxonase, an improved lipid profile, and improved liver and kidney functions for a treated diabetic group with CRB. Metabolite profiling of CRB was determined by UPLC-ESI-QTOF-MS and tandem MS/MS. Twenty-three chromatographic peaks were identified, which were classified into phenolic compounds and amino acids. The characterized flavonoids were apigenin and luteolin derivatives.

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“…Pancreatic α-amylase is one of the most common amylase enzymes. Suppressing α-amylase enzyme activity helps to reduce hyperglycemia [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Quality Control, Anti-Hyperglycemic, and Anti-Inflammatory Assessment of Colvillea racemosa Leaves Using In Vitro, In Vivo Investigations and Its Correlation with the Phytoconstituents Identified via LC-QTOF-MS and MS/MS

Hafeez

Gindi

Hetta

et al. 2022

Plants

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“…Then, αglucosidase catalyzes hydrolysis to produce monosaccharides such as glucose and fructose from disaccharides. This event prevents the absorption of glucose into the blood circulatory system [37,38]. The strategy to decrease carbohydrate digestibility by inhibiting the activity of these two hydrolyzing enzymes to control blood sugar is considered a prophylactic treatment of type 2 DM [10].…”

Section: Discussionmentioning

confidence: 99%

Machine Learning and In Vitro Chemical Screening of Potential α-Amylase and α-Glucosidase Inhibitors from Thai Indigenous Plants

et al. 2022

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Diabetes mellitus is a major predisposing factor for cardiovascular disease and mortality. α-Amylase and α-glucosidase enzymes are the rate-limiting steps for carbohydrate digestion. The inhibition of these two enzymes is clinically used for the treatment of diabetes mellitus. Here, in vitro study and machine learning models were employed for the chemical screening of inhibiting the activity of 31 plant samples on α-amylase and α-glucosidase enzymes. The results showed that the ethanolic twig extract of Pinus kesiya had the highest inhibitory activity against the α-amylase enzyme. The respective ethanolic extract of Croton oblongifolius stem, Parinari anamense twig, and Polyalthia evecta leaf showed high inhibitory activity against the α-glucosidase enzyme. The classification analysis revealed that the α-glucosidase inhibitory activity of Thai indigenous plants was more predictive based on phytochemical constituents, compared with the α-amylase inhibitory activity (1.00 versus 0.97 accuracy score). The correlation loading plot revealed that flavonoids and alkaloids contributed to the α-amylase inhibitory activity, while flavonoids, tannins, and reducing sugars contributed to the α-glucosidase inhibitory activity. In conclusion, the ethanolic extracts of P. kesiya, C. oblongifolius, P. anamense, and P. evecta have the potential for further chemical characterization and the development of anti-diabetic recipes.

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“…The methanol and ethanol extract revealed IC50 67.64 ± 0.36 µg/mL and 67.46 ± 1.23 µg/mL. The α-amylase enzyme played a role in breaking down the polysaccharides into glucose and maltose [28]. B. racemosa leaves contained high phenolic and flavonoid content.…”

Section: Antidiabetesmentioning

confidence: 97%

The Review of Baccaurea racemosa: Neglected Plants, but Potential to be Developed

Permatasari¹,

Riyanto²,

Rohman³

2021

Advances in Health Sciences Research

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The Baccaurea racemosa was the plant that spread in the Southeast Asia. B. racemosa fruit had a sour taste, so that the economy of this plant was low. B. racemosa has almost been forgotten in people. If this plant bears fruit, the fruit is often neglected. If the fruit is sold, the price will be low even though many studies reported the biological activities of B. racemosa. This review aims to inform the identification of B. racemosa, its traditional uses, its biologic activities, and the detected compounds. The relevant source was searched in PubMed, Scopus, and google scholar. Searching of the article was using the highlight keywords, such as Baccaurea racemosa, traditional uses of Baccaurea racemosa, and the biological activity of Baccaurea racemosa. The relevant article that was downloaded and reviewed was analyzed. B. racemosa was the tall tree, had the yellowish-green fruit skin, had the pink to a purplish pulp with high water content. All part of B. racemosa was determined their antioxidant activity. The leaves of B. racemosa had the potent antioxidant activity. The DPPH was the popular method to analyze the antioxidant activity of B. racemosa. The leaves were traditionally utilized for antiinflammation. Other biological activities of B. racemosa were antidiabetic and antibacterial. The detected compounds in B. racemosa were phenolic, flavonoid, fatty acids, and terpenoid groups. This review will help the researcher develop the utilization and research of B. racemosa in terms of biological activity and its secondary metabolite.

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Alpha‐amylase as molecular target for treatment of diabetes mellitus: A comprehensive review

Cited by 98 publications

References 90 publications

Quality Control, Anti-Hyperglycemic, and Anti-Inflammatory Assessment of Colvillea racemosa Leaves Using In Vitro, In Vivo Investigations and Its Correlation with the Phytoconstituents Identified via LC-QTOF-MS and MS/MS

Quality Control, Anti-Hyperglycemic, and Anti-Inflammatory Assessment of Colvillea racemosa Leaves Using In Vitro, In Vivo Investigations and Its Correlation with the Phytoconstituents Identified via LC-QTOF-MS and MS/MS

Machine Learning and In Vitro Chemical Screening of Potential α-Amylase and α-Glucosidase Inhibitors from Thai Indigenous Plants

The Review of Baccaurea racemosa: Neglected Plants, but Potential to be Developed

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