ALP gene expression in cDNA samples from bone tissue engineering using a HA/TCP/Chitosan scaffold

Stephanie, N.; Katarina, H.; Amir, Lisa Rinanda; Gunawan, Harun Asjiq

doi:10.1088/1742-6596/884/1/012112

Cited by 2 publications

(2 citation statements)

References 18 publications

(22 reference statements)

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“…Also, it has an imminent role at initial phase of bone formation. For this reason, ALP is often recognized as a main bone marker for bone formation 52 . Therefore, in this study, ALP activity of scaffolds was evaluated after elapsing of 7, 14, and 21 days.…”

Section: Resultsmentioning

confidence: 99%

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Incorporation of graphene oxide and calcium phosphate in the PCL/PHBV core‐shell nanofibers as bone tissue scaffold

Mollaqasem

Asefnejad

Nourani

et al. 2020

J of Applied Polymer Sci

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Bone tissue scaffolds should have both desired mechanical stability and cell activities including biocompatibility, cell differentiation, and maturation. Also, suitable mineralization is another key factor for these materials. Hence, in current work, in order to achieve a scaffold with desired mechanical and bioactivity properties, core-shell nanofibers based on the polycaprolactone and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) with different concentration of graphene oxide (GO) (0.5, 1, and 1.5 wt%) and calcium phosphate (CP) (1 and 3 wt%) were prepared to utilize as bone scaffold. Microstructure of nanofibers observed by field emission scanning electron microscope (FE-SEM) and results exhibited that the most of nanofibers had 270-500 nm diameter. Attenuated total reflectance Fourier transform infrared spectroscopy and energy dispersive X-ray evaluations verified appearance of GO and CP into the electrospun scaffolds (ES). Transmission electron microscopy analysis endorsed core-shell structure of nanofibers. X-ray diffraction study moreover determination of semicrystalline structure, verified presence of GO and CaPO 4 into the nanofibers. Water contact angle demonstrates that, ES2 and ES3 situated in suitable domain of hydrophilicity. Tensile analysis determined that, ES2, ES3, and ES4 had the highest mechanical properties for use as bone scaffold. Cell viability assessment confirmed biocompatibility of scaffold during 7 days. Alkaline phosphatase and alizarin red staining exhibited maturating and differentiating of osteocytes after 21 days seeding on the scaffolds.

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Section: Resultsmentioning

confidence: 99%

“…For this reason, ALP is often recognized as a main bone marker for bone formation. 52 Therefore, in this study, ALP activity of scaffolds was evaluated after elapsing of 7, 14, and 21 days. As depicted in Figure 13, the maximum value of ALP activity recorded after 14 days, and subsequently decreased after 21 days.…”

Section: Evaluation Of Cells Alp Activitymentioning

confidence: 99%