Aloe barbadensis Miller leaf exudate is a potential treatment for bovine mastitis

Abstract

Discuss this article AbstractMiller is a well-known phytotherapeutic, and Background: Aloe barbadensis parts of its leaves are used for a wide range of medicinal purposes. This study seeks to assess the antimicrobial and cytotoxic effects of leaf exudate in vitro (LE) from leaves against and MAC-T A. barbadensis Staphylococcus aureus bovine mammary epithelial cells.Seasonal LE samples were collected, and the effect on total Methods: phenolic and aloin contents was determined. Antimicrobial activity of LE was evaluated using the broth microdilution method, and toxicity to MAC-T cells was determined by MTT assay.Samples collected during different seasons of the year showed a Results: seasonal effect on the chemical profile of LE (P<0.05). However, despite these chemical variations, we found no differences in antimicrobial activity against S.For all studied samples, the minimum inhibitory concentration (MIC) aureus. was 1,000 µg/ml. Furthermore, we found an elevated cytotoxic effect of LE on MAC-T cells with a significant reduction in cellular viability at 7.8 µg/ml (P<0.05) and an IC50 of 91.89 µg/ml.Despite the antimicrobial effects of LE, the high toxicity for Conclusions: MAC-T cells suggests that it is unsuitable for intramammary use, but does have potential as a topical antimicrobial.