Alocilja Magnetic Nanoparticles capture Escherichia coli O157:H7 Isolates

Lim, Dodge R.; Villame, Rovi Gem; Quiñones, Gloria June; Vera, Dave de; Notorio, Rebecca; Fernando, Lilia M.; Alocilja, Evangelyn C.

doi:10.21141/pjp.2017.018

Cited by 4 publications

(6 citation statements)

References 8 publications

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“…The MNP experiment (set A) was carried out as described by [22] with some modifications. Briefly, a volume of ten microliters (10 µL) of 5 mg/mL MNP concentration was pipetted into each tube, containing serially diluted spiked fecal samples ranging from 10 −1 (1.5 × 10 4 CFU/mL) to 10 −5 (1.5 CFU/mL) dilutions.…”

Section: Capture Efficiency Of Mnpsmentioning

confidence: 99%

“…The colonies on each plate for all serial dilutions (including sets A and B) were enumerated. The capture efficiency (CE) was therefore determined by dividing the log10 of the number of cells captured by the log10 of the number of cells before capture [22]:…”

Section: Capture Efficiency Of Mnpsmentioning

confidence: 99%

“…The MNP and GNP biosensors used in this study have already been validated in similar experiments using other organisms and food matrices [20,[22][23][24]. However, the performance of the biosensor in bovine fecal samples has not yet been explored.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, this study determines the capture efficiency of the MNPs, determines the analytical specificity and sensitivity/limit of detection (LOD) of the GNPs plasmonic/colorimetric biosensor, and finally, evaluates the performance of the GNPs biosensor on naturally contaminated bovine fecal samples. The MNP and GNP biosensors used in this study have already been validated in similar experiments using other organisms and food matrices [20,[22][23][24]. However, the performance of the biosensor in bovine fecal samples has not yet been explored.…”

Section: Introductionmentioning

confidence: 99%

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Gold Nanoparticle-Based Plasmonic Detection of Escherichia coli, Salmonella enterica, Campylobacter jejuni, and Listeria monocytogenes from Bovine Fecal Samples

Ghazy,

Nyarku,

Faraj

et al. 2024

Microorganisms

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Current diagnostic methods for detecting foodborne pathogens are time-consuming, require sophisticated equipment, and have a low specificity and sensitivity. Magnetic nanoparticles (MNPs) and plasmonic/colorimetric biosensors like gold nanoparticles (GNPs) are cost-effective, high-throughput, precise, and rapid. This study aimed to validate the use of MNPs and GNPs for the early detection of Escherichia coli O157:H7, Salmonella enterica spp., Campylobacter jejuni, and Listeria monocytogenes in bovine fecal samples. The capture efficiency (CE) of the MNPs was determined by using Salmonella Typhimurium (ATCC_13311) adjusted at an original concentration of 1.5 × 108 CFU/mL. One (1) mL of this bacterial suspension was spiked into bovine fecal suspension (1 g of fecal sample in 9 mL PBS) and serially diluted ten-fold. DNA was extracted from Salmonella Typhimurium to determine the analytical specificity and sensitivity/LOD of the GNPs. The results showed that the CE of the MNPs ranged from 99% to 100% and could capture as little as 1 CFU/mL. The LOD of the GNPs biosensor was 2.9 µg/µL. The GNPs biosensor was also tested on DNA from 38 naturally obtained bovine fecal samples. Out of the 38 fecal samples tested, 81.6% (31/38) were positive for Salmonella enterica spp., 65.8% (25/38) for C. jejuni, 55.3% (21/38) for L. monocytogenes, and 50% (19/38) for E. coli O157:H7. We have demonstrated that MNP and GNP biosensors can detect pathogens or their DNA at low concentrations. Ensuring food safety throughout the supply chain is paramount, given that these pathogens may be present in cattle feces and contaminate beef during slaughter.

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Section: Capture Efficiency Of Mnpsmentioning

confidence: 99%

Section: Capture Efficiency Of Mnpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gold Nanoparticle-Based Plasmonic Detection of Escherichia coli, Salmonella enterica, Campylobacter jejuni, and Listeria monocytogenes from Bovine Fecal Samples

Ghazy,

Nyarku,

Faraj

et al. 2024

Microorganisms

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“…We have shown that GMNP attach to the mycobacteria cell wall, allowing the capture, detection, and quantification of AFB in artificial and natural sputum samples [ 20 ]. GMNP have also been used to extract, isolate, and concentrate other pathogenic bacteria in food, such as Salmonella enterica, Bacillus cereus, Staphylococcus aureus, Escherichia coli O157:H7, Listeria monocytogenes , and other human pathogens [ 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Tween 80 Improves the Acid-Fast Bacilli Quantification in the Magnetic Nanoparticle-Based Colorimetric Biosensing Assay (NCBA)

et al. 2022

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Despite its reduced sensitivity, sputum smear microscopy (SSM) remains the main diagnostic test for detecting tuberculosis in many parts of the world. A new diagnostic technique, the magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was optimized by evaluating different concentrations of glycan-functionalized magnetic nanoparticles (GMNP) and Tween 80 to improve the acid-fast bacilli (AFB) count. Comparative analysis was performed on 225 sputum smears: 30 with SSM, 107 with NCBA at different GMNP concentrations, and 88 with NCBA-Tween 80 at various concentrations and incubation times. AFB quantification was performed by adding the total number of AFB in all fields per smear and classified according to standard guidelines (scanty, 1+, 2+ and 3+). Smears by NCBA with low GMNP concentrations (≤1.5 mg/mL) showed higher AFB quantification compared to SSM. Cell enrichment of sputum samples by combining NCBA-GMNP, incubated with Tween 80 (5%) for three minutes, improved capture efficiency and increased AFB detection up to 445% over SSM. NCBA with Tween 80 offers the opportunity to improve TB diagnostics, mainly in paucibacillary cases. As this method provides biosafety with a simple and inexpensive methodology that obtains results in a short time, it might be considered as a point-of-care TB diagnostic method in regions where resources are limited.

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Emerging nano-biosensing with suspended MNP microbial extraction and EANP labeling

Matta

Alocilja

2018

Biosensors and Bioelectronics

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Alocilja Magnetic Nanoparticles capture Escherichia coli O157:H7 Isolates

Cited by 4 publications

References 8 publications

Gold Nanoparticle-Based Plasmonic Detection of Escherichia coli, Salmonella enterica, Campylobacter jejuni, and Listeria monocytogenes from Bovine Fecal Samples

Gold Nanoparticle-Based Plasmonic Detection of Escherichia coli, Salmonella enterica, Campylobacter jejuni, and Listeria monocytogenes from Bovine Fecal Samples

Tween 80 Improves the Acid-Fast Bacilli Quantification in the Magnetic Nanoparticle-Based Colorimetric Biosensing Assay (NCBA)

Emerging nano-biosensing with suspended MNP microbial extraction and EANP labeling

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