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Riffaud, Christine; Morris, Cindy E.

doi:10.1023/a:1019919627886

Cited by 30 publications

(5 citation statements)

References 25 publications

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“…Immunofluorescence can detect antigens using a microscope that excites the fluorescent molecules and captures their emitted light [157]. In plant pathogen detection and diagnosis, immunofluorescence can be used to identify the presence of specific pathogen-associated molecular patterns (PAMPs) or effector proteins, which are secreted by pathogens and contribute to disease development (Figure 3) [102,124,125]. Some advantages of immunofluorescence for plant pathogen detection and diagnosis are the following: (1) High sensitivity: Immunofluorescence is highly sensitive and can detect low levels of specific molecules in a sample.…”

Section: Detection and Diagnosis Of P Syringae With Serological Methodsmentioning

confidence: 99%

“…(3) Interference from the other molecules: Immunofluorescence signals can be masked or confused by the other molecules in the sample that cross-react with the antibodies. ( 4) Need for specialized equipment: Immunofluorescence requires the use of specialized equipment, such as fluorescence microscopes, which can be expensive and may not be available in all laboratories (Table 2) [102,124,125].…”

Section: Detection and Diagnosis Of P Syringae With Serological Methodsmentioning

confidence: 99%

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Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Yang

Zhao

Gao

et al. 2023

Plants

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Plant diseases caused by the pathogen Pseudomonas syringae are serious problems for various plant species worldwide. Accurate detection and diagnosis of P. syringae infections are critical for the effective management of these plant diseases. In this review, we summarize the current methods for the detection and diagnosis of P. syringae, including traditional techniques such as culture isolation and microscopy, and relatively newer techniques such as PCR and ELISA. It should be noted that each method has its advantages and disadvantages, and the choice of each method depends on the specific requirements, resources of each laboratory, and field settings. We also discuss the future trends in this field, such as the need for more sensitive and specific methods to detect the pathogens at low concentrations and the methods that can be used to diagnose P. syringae infections that are co-existing with other pathogens. Modern technologies such as genomics and proteomics could lead to the development of new methods of highly accurate detection and diagnosis based on the analysis of genetic and protein markers of the pathogens. Furthermore, using machine learning algorithms to analyze large data sets could yield new insights into the biology of P. syringae and novel diagnostic strategies. This review could enhance our understanding of P. syringae and help foster the development of more effective management techniques of the diseases caused by related pathogens.

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Section: Detection and Diagnosis Of P Syringae With Serological Methodsmentioning

confidence: 99%

Section: Detection and Diagnosis Of P Syringae With Serological Methodsmentioning

confidence: 99%

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Yang

Zhao

Gao

et al. 2023

Plants

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“…In woody perennial plants, bacteria over-winter in dormant buds [25]. Plant JOURNAL DE PHYSIQUE IV pathogenic bacteria can also over-winter in lake water used for irrigation [26]. Bacteria from plant surfaces can become part of atmospheric aerosols and then land on other plants via dry deposition.…”

Section: Origin and Fate Of Leaf Surface Colonists: Comings And Goingsmentioning

confidence: 99%

Ice nucleation active bacteria and their potential role in precipitation

Morris¹,

2004

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Certain bacteria that are commonly found on plants have the capacity to catalyze the freezing of supercooled water at temperatures as warm as −1 • C. This is conferred by a protein in the outer membrane of the bacterial cell. Because of the abundance of these bacteria and the warm temperature at which they function as ice nuclei, they are considered to be among the most active of the naturally-occurring ice nuclei. As plant pathogens, antagonists of plant pathogens and as causal agents of frost damage, these bacteria have wellstudied interactions with plants. Here we propose that these bacteria also play a role in atmospheric processes leading to rain, given that they are readily disseminated into the atmosphere and have been found in clouds at altitudes of several kilometers. That they participate in a sort of biological cycle of precipitation-whereby they are transported into clouds from plant canopies and incite rain thereby causing favorable conditions for their growth on plant surfaces-was proposed about 20 years ago. Today, sufficient evidence and meteorological tools have emerged to re-ignite interest in bioprecipitation and in the ways in which plants play a role as cloud seeders. 88 JOURNAL DE PHYSIQUE IV 2. THE BIOLOGICAL COMPONENT OF AEROSOLS Outside of urban zones, the air over continents contains 3 × 10 9 to 5 × 10 10 particles/m 3. Mineral dusts are abundant in atmospheric aerosols. In polluted regions a major component of these aerosols can be soot. But, anyone afflicted with allergies to pollen knows that there is a biological component to atmospheric aerosols. In fact, up to 25 % of the insoluble part of aerosols can be of biological origin [1]. Pollen is only one of the biological components of aerosols and is only one of the types of particles that plants contribute to the atmosphere. The seemingly abiotic atmosphere that surrounds our planet is a bouillon of biological bits including bacteria; protozoa; spores of fungi, ferns and mosses; virus particles; parts of insects and dust mites; algae and pollen grains. In some cases, these biotic particles are dead debris that are picked up into the air. But often, aerial dissemination is an integral part of the life cycle of organisms, a sort of boulevard to opportunities for species out-crossing and to supplemental food and water resources. At altitudes below about 15 m, there are hundreds to thousands of particles of biological origin per m 3 of air. Over a suburban region the quantity of microorganisms, for example, in the air has been observed to be as high as 7000 culturable propagules of fungi and 1600 cultural bacteria/m 3 [2]. The bulk of the popular interest in aerobiology concerns the presence-in the air we breath-of biological particles with direct negative impacts on human health such as allergens, causal agents of pneumonia and other lung diseases and of septicemias in hospital environments. The Andersen sampler, widely used in studies of aerobiology, was conceived to simulate uptake of particles by the human respiratory system [3]. This...

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“…A rapid and accurate method was desired to be developed to identify the source of V . mali infection, which can be used to assist in the reduction of t the primary inoculum and the development of disease control strategies 9 .…”

Section: Introductionmentioning

confidence: 99%

Latent Infection of Valsa mali in the Seeds, Seedlings and Twigs of Crabapple and Apple Trees is a Potential Inoculum Source of Valsa Canker

Meng

Han

et al. 2019

Sci Rep

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A real-time quantitative PCR assay using a species-specific primer pair was developed to rapidly and accurately quantify Valsa mali , the causative pathogen of apple Valsa canker (AVC), in crabapple seeds, crabapple seedlings, apple twigs and apple seeds. Surveys were conducted in different regions, and crabapple or apple seeds were collected for V . mali detection by qPCR assay. Our results showed that 12.87% to 49.01% of crabapple seeds collected from different regions were positive for V . mali . The exopleura and endopleura were the two major areas of V . mali infection in crabapple seeds. The presence of V . mali infection in crabapple seeds was also confirmed by a high-throughput sequencing approach. With the growth of crabapple seedlings, the concentration of V . mali gDNA in crabapple seedlings gradually increased until eight or more leaf blades emerged. One-year-old twigs from an apple scion nursery were infected with V . mali , and only apple seeds from infected apple trees showing evident Valsa canker symptoms carried V . mali . In conclusion, this study reports that crabapple seeds and apple seeds carried V . mali as latent inoculum sources. V . mali infected not only apple tissues but also crabapple seedlings, which are the rootstocks of apple trees. This study indicated that the inoculum sources for AVC vary. Application of a novel qPCR assay can potentially improve the accuracy of early diagnosis, and is helpful to reveal the epidemic regularity of AVC.

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Untitled

Cited by 30 publications

References 25 publications

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Ice nucleation active bacteria and their potential role in precipitation

Latent Infection of Valsa mali in the Seeds, Seedlings and Twigs of Crabapple and Apple Trees is a Potential Inoculum Source of Valsa Canker

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