Allyl-, butyl- and phenylethyl-isothiocyanate activate Nrf2 in cultured fibroblasts

Ernst, Insa M. A.; Wagner, Anika E.; Schuemann, C.; Storm, Niels; Höppner, Wolfgang; Döring, Frank; Stocker, Achim; Rimbach, Gerald

doi:10.1016/j.phrs.2010.11.005

Cited by 83 publications

(61 citation statements)

References 36 publications

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“…For example, SF, PEITC, BITC, and AITC were generally shown to increase transcript and protein abundance of γ-GCS, HO-1, and NQO1 when applied to NIH3T3 fibroblast cells at the lowest dose tested (5 µM) [74]. A similar study using the same four ITCs and mouse skin papilloma cells found analogous results, with significant increases in NQO1 and GST activity.…”

Section: In Vitro Evidence Of Are-dependent Gene Induction By Itcsmentioning

confidence: 90%

The Role of Glucosinolate Hydrolysis Products from Brassica Vegetable Consumption in Inducing Antioxidant Activity and Reducing Cancer Incidence

Becker

Juvik

2016

Diseases

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The bioactivity of glucosinolates (GSs), and more specifically their hydrolysis products (GSHPs), has been well documented. These secondary metabolites evolved in the order Brassicales as plant defense compounds with proven ability to deter or impede the growth of several biotic challenges including insect infestation, fungal and bacterial infection, and competition from other plants. However, the bioactivity of GSHPs is not limited to activity that inhibits these kingdoms of life. Many of these compounds have been shown to have bioactivity in mammalian systems as well, with epidemiological links to cancer chemoprevention in humans supported by in vitro, in vivo, and small clinical studies. Although other chemopreventive mechanisms have been identified, the primary mechanism believed to be responsible for the observed chemoprevention from GSHPs is the induction of antioxidant enzymes, such as NAD(P)H quinone reductase (NQO1), heme oxygenase 1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), and glutathione S transferases (GSTs), through the Keap1-Nrf2-ARE signaling pathway. Induction of this pathway is generally associated with aliphatic isothiocyanate GSHPs, although some indole-derived GSHPs have also been associated with induction of one or more of these enzymes.

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Section: In Vitro Evidence Of Are-dependent Gene Induction By Itcsmentioning

confidence: 90%

The Role of Glucosinolate Hydrolysis Products from Brassica Vegetable Consumption in Inducing Antioxidant Activity and Reducing Cancer Incidence

Becker

Juvik

2016

Diseases

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“…Although NIH 3T3 cells do not fully reflect the cellular metabolism of cardiomyocytes, they comprise a valuable experimental tool to screen for Nrf2-inducing activity of plant bioactives. 55 All cell culture plasticware was purchased from Sarstedt (Nuembrecht, Germany) unless otherwise stated. For cell culture experiments, the olive oil phenolics were dissolved in dimethylsulfoxide (DMSO; Carl Roth, Karlsruhe, Germany), and stock solutions were stored at -80°C until usage.…”

Section: Rna Isolation and Real-time Quantitative Rt-pcrmentioning

confidence: 99%

A Diet Rich in Olive Oil Phenolics Reduces Oxidative Stress in the Heart of SAMP8 Mice by Induction of Nrf2-Dependent Gene Expression

Bayram

Özçelik

Grimm

et al. 2012

Rejuvenation Research

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112

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A Mediterranean diet rich in olive oil has been associated with health benefits in humans. It is unclear if and to what extent olive oil phenolics may mediate these health benefits. In this study, we fed senescence-accelerated mouse-prone 8 (SAMP8, n = 11 per group) semisynthetic diets with 10% olive oil containing either high (HP) or low amounts of olive oil phenolics (LP) for 4.5 months. Mice consuming the HP diet had significantly lower concentrations of the oxidative damage markers thiobarbituric acid-reactive substances and protein carbonyls in the heart, whereas proteasomal activity was similar in both groups. Nrf2-dependent gene expression may be impaired during the aging process. Therefore, we measured Nrf2 and its target genes glutathione-S-transferase (GST), c-glutamyl cysteine synthetase (c-GCS), nicotinamide adenine dinucleotide phosphate [NAD(P)H]:quinone oxidoreductase (NQO1), and paraoxonase-2 (PON2) in the hearts of these mice. Nrf2 as well as GST, c-GCS, NQO1, and PON2 mRNA levels were significantly higher in heart tissue of the HP as compared to the LP group. The HP-fed mice had significantly higher PON1 activity in serum compared to those receiving the LP diet. Furthermore, HP feeding increased relative SIRT1 mRNA levels. Additional mechanistic cell culture experiments were performed, and they suggest that the olive oil phenolic hydroxytyrosol present in the HP oil may be responsible for the induction of Nrf2-dependent gene expression and the increase in PON activity. In conclusion, a diet rich in olive oil phenolics may prevent oxidative stress in the heart of SAMP8 mice by modulating Nrf2-dependent gene expression.

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“…4). It has been stated in the literature that kinases, including ERK1/2 and JNK, activate the transcription factor Nrf2 via phosphorylation, resulting in the stabilization of Nrf2 and, consequently, in its dissociation from its inhibitor protein Keap1 [30,34,42,43]. By applying the small-molecule inhibitors of the abovementioned kinases to our GER+MYR-treated HT-29 cells, we also detected changes in HO-1 gene expression (Fig.…”

Section: Discussionmentioning

confidence: 56%

“…The nuclear cell extracts, for Nrf2 detection, and the whole cell extracts, for HO-1 and ERK1/2/p-ERK1/2, JNK/p-JNK and p38/p-p38 detection, were prepared as described previously [30,31]. For the nuclear extracts, the cells were treated for 6 h with 25 μmol/L GER, 25 μmol/L GER plus 1.6 μl of MYR, 25 μmol/L SIN, 25 μmol/L SIN plus 1.6 μl of MYR, 25 μmol/L GRA, 25 μmol/L GRA plus 1.6 μl of MYR, 25 μmol/L GST and 25 μmol/L GST plus 1.6 μl of MYR or SFN (as a positive control).…”

Section: Western Blottingmentioning

confidence: 99%

Myrosinase-treated glucoerucin is a potent inducer of the Nrf2 target gene heme oxygenase 1 — studies in cultured HT-29 cells and mice

Wagner

Sturm

Piegholdt

et al. 2015

The Journal of Nutritional Biochemistry

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Allyl-, butyl- and phenylethyl-isothiocyanate activate Nrf2 in cultured fibroblasts

Cited by 83 publications

References 36 publications

The Role of Glucosinolate Hydrolysis Products from Brassica Vegetable Consumption in Inducing Antioxidant Activity and Reducing Cancer Incidence

The Role of Glucosinolate Hydrolysis Products from Brassica Vegetable Consumption in Inducing Antioxidant Activity and Reducing Cancer Incidence

A Diet Rich in Olive Oil Phenolics Reduces Oxidative Stress in the Heart of SAMP8 Mice by Induction of Nrf2-Dependent Gene Expression

Myrosinase-treated glucoerucin is a potent inducer of the Nrf2 target gene heme oxygenase 1 — studies in cultured HT-29 cells and mice

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