Myrosinase-treated glucoerucin is a potent inducer of the Nrf2 target gene heme oxygenase 1 — studies in cultured HT-29 cells and mice

Wagner, Anika E.; Sturm, Christine; Piegholdt, Stefanie; Wolf, Insa M. A.; Esatbeyoglu, Tuba; Nicola, Gina Rosalinda De; Iori, Renato; Rimbach, Gerald

doi:10.1016/j.jnutbio.2015.01.004

Cited by 22 publications

(15 citation statements)

References 43 publications

(53 reference statements)

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“…Another aliphatic ITC common to Brassica crops that is gaining attention is erucin, from the precursor GS glucoerucin. This ITC was recently found to induce HO-1 expression in vitro and in vivo , possibly acting through a similar mechanism of Nrf2 activation as PEITC [96]. Interestingly, erucin can also undergo interconversion to SF in vivo , although the interconversion ratio of SF and erucin is not consistent across individuals [156].…”

Section: Response Of Ao and Pii Enzymes To Itc Treatment In Animalmentioning

confidence: 99%

The Role of Glucosinolate Hydrolysis Products from Brassica Vegetable Consumption in Inducing Antioxidant Activity and Reducing Cancer Incidence

Becker

Juvik

2016

Diseases

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The bioactivity of glucosinolates (GSs), and more specifically their hydrolysis products (GSHPs), has been well documented. These secondary metabolites evolved in the order Brassicales as plant defense compounds with proven ability to deter or impede the growth of several biotic challenges including insect infestation, fungal and bacterial infection, and competition from other plants. However, the bioactivity of GSHPs is not limited to activity that inhibits these kingdoms of life. Many of these compounds have been shown to have bioactivity in mammalian systems as well, with epidemiological links to cancer chemoprevention in humans supported by in vitro, in vivo, and small clinical studies. Although other chemopreventive mechanisms have been identified, the primary mechanism believed to be responsible for the observed chemoprevention from GSHPs is the induction of antioxidant enzymes, such as NAD(P)H quinone reductase (NQO1), heme oxygenase 1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), and glutathione S transferases (GSTs), through the Keap1-Nrf2-ARE signaling pathway. Induction of this pathway is generally associated with aliphatic isothiocyanate GSHPs, although some indole-derived GSHPs have also been associated with induction of one or more of these enzymes.

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Section: Response Of Ao and Pii Enzymes To Itc Treatment In Animalmentioning

confidence: 99%

The Role of Glucosinolate Hydrolysis Products from Brassica Vegetable Consumption in Inducing Antioxidant Activity and Reducing Cancer Incidence

Becker

Juvik

2016

Diseases

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“…Many studies have demonstrated that high dose of D‐galactose can result in the accumulation of galactitol, leading to osmotic stress, generation of ROS (Hsieh, Wu, & Hu, ; Li et al., ; Yi, Fu, Li, Gao, & Zhang, ), and formation of advanced glycation end products within cells, which in turn result in oxidative stress and cellular damage in the tissues of living organisms (Hamada, Fujii, & Fukagawa, ; Hsieh et al., ; Wu et al., ; Yi et al., ). Under normal conditions, nuclear factor erythroid‐2‐related factor 2 (Nrf2) is a transcription factor that binds to antioxidant response elements in the promoter regions of many antioxidative enzymes and phase II detoxifying enzymes (Arlt et al., ; Jones et al., ; Li et al., , ; Qu et al., ; Ren et al., ; Sun, Zhang, Chan, & Zhang, ; Wagner et al., ; Zhang, An et al., ).…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus plantarum AR501 Alleviates the Oxidative Stress of D‐Galactose‐Induced Aging Mice Liver by Upregulation of Nrf2‐Mediated Antioxidant Enzyme Expression

Lin

Xia

Wang

et al. 2018

Journal of Food Science

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Lactic acid bacteria (LAB) have been used as ingredients of functional foods to promote health and prevent diseases because of their beneficial effects. This study aimed to investigate the antioxidative effects of LAB on the hepatotoxicity in D-galactose-induced aging mice. LAB were isolated from the traditional Chinese fermented foods and screened by the tolerance of hydrogen peroxide (H O ). Male ICR (Institute of Cancer Research) mice were subcutaneously injected with D-galactose for 5 weeks and then gastric gavage with LAB for 6 weeks. The results showed that Lactobacillus plantarum AR113 and AR501, and Pediococcu pentosaceus AR243 could tolerate up to 1.5 mM H O in vitro, and they could live through simulated gastrointestinal tract (GIT) to colonizing the GIT of host. In vivo, oral administration of L. plantarum AR113 and AR501 improved the antioxidant status of D-galactose-induced oxidative stress mice such as alleviated liver damages and reduced abnormal activities of superoxide dismutase, glutathione peroxidase, and catalase to normal levels. In addition, L. plantarum AR501 markedly elevated the gene expression of nuclear factor erythroid-2-related factor 2 and upregulated the expressions of several antioxidant genes such as glutathione reductase, glutathione S-transferase, glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit, and NAD(P)H quinone oxidoreductase 1 in the liver of an aging mice. Therefore, L. plantarum AR501 could be a good candidate for producing antiaging functional foods.

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“…A cluster of genes is induced by Nrf-2. Hereby, we confirmed that a target Gst-α was responsible for reduction of the toxicity of oxidative stress by rapid clearance of 4-HNE, although our study did not exclude a role for other Nrf-2 targets, such as HO-1 [25], ALDH [25]. Future studies will be necessary to determine the capacity of these molecules to convert 4-HNE to less reactive chemical agent.…”

Section: Discussionmentioning

confidence: 49%

Nrf-2/Gst-α mediated imatinib resistance through rapid 4-HNE clearance

Wang

Chen

et al. 2017

Experimental Cell Research

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The advent of imatinib mesylate (IM) has dramatically improved the outcome of patients with chronic myeloid leukemia, but drug resistance, particularly in advanced stage of disease, portents eventual relapse and progression. To identify the candidate molecule responsible for resistance during IM treatment, an IM-resistant K562 cell line was generated by culturing in gradually increasing dose of IM. The expression of Nrf-2 and its downstream target, Gst-α, were significantly induced in these cells. GST-α, in turn, mediated cell survival by maintaining intracellular low level of 4-HNE. Inhibition of Nrf-2 effectively reduced the expression of Gst-α, resulting in accumulation of 4-HNE and elevated sensitiveness to IM. Moreover, in IM-sensitive K562 cells enforced Gst-α expression strikingly protected cells from the insult of IM. Finally, we also examined the levels of Nrf-2 in clinical bone morrow samples. Nrf-2 and Gst-α were more abundant in bone morrow of CML patients compared with that of healthy donors. In addition, Nrf-2 and Gst-α were further up-regulated in samples of patients with weak response to IM. In conclusion, our study shows that rapid clearance of 4-HNE by Nrf-2/GST may represents a novel molecular basis of IM resistance in CML.

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Myrosinase-treated glucoerucin is a potent inducer of the Nrf2 target gene heme oxygenase 1 — studies in cultured HT-29 cells and mice

Cited by 22 publications

References 43 publications

The Role of Glucosinolate Hydrolysis Products from Brassica Vegetable Consumption in Inducing Antioxidant Activity and Reducing Cancer Incidence

The Role of Glucosinolate Hydrolysis Products from Brassica Vegetable Consumption in Inducing Antioxidant Activity and Reducing Cancer Incidence

Lactobacillus plantarum AR501 Alleviates the Oxidative Stress of D‐Galactose‐Induced Aging Mice Liver by Upregulation of Nrf2‐Mediated Antioxidant Enzyme Expression

Nrf-2/Gst-α mediated imatinib resistance through rapid 4-HNE clearance

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