Allozyme variation in rye, Secale cereale L.

Abstract

Eleven samples of eight European commercial varieties of winter rye were examined at eight polymorphic enzyme loci. Genotype frequencies fitted Hardy-Weinberg expectations at all loci in all samples studied. Of the total genetic diversity recorded at the 8 loci, only 7% was expressed between varieties. Allele frequency differences between varieties were, however, sufficient to allow a characterization of each variety by a specific set of allele frequencies. Using subsets of the original data, it could be demonstrated that all pairs of varieties but one still showed significant allozyme differences, when only 4 loci were screened in samples half the original size of 200 individuals. Even when only one locus was analyzed, all varieties but two were distinguishable, but this "diagnostic" locus was not identical in all pairwise comparisons.

A comparative analysis of the genetic relationships between rye cultivars using RFLP and RAPD markers

“…Though there have been some concerns about the reliability of RAPD markers and their ability to detect variation at high frequency (Riedy et al ., 1992 ;Ellsworth et al ., 1993), the optimization of experimental protocols for each particular situation have allowed RAPD analyses to provide information on taxonomic and phylogenetic relationships in a large number of plant genera and species, 'Carokurz' 'Kustro' 'Kungs II' 'Carogold' 'Halo' Fig. 1 . Relationships between some of the rye varieties studied based on Adam et al . (1987) .…”

“…The aim of this study was to compare the groups of cultivars, based on genetic similarity, generated by each type of marker. Since previous estimates have been made of the genetic distances between these cultivars, based on isozymes analysis (Adam et al ., 1987), the comparison of techniques could be further extended .…”

Characterization of 43 Populus nigra L. clones representing selections, cultivars and botanical varieties based on their multilocus allozyme genotypes

“…nigra and other poplars . This method of clonal identification is reliable, precise, simple, fast, objective and inexpensive and can be applied to living (Bassiri & Adams, 1978) or based on the presence or absence of each allozyme (Oliver & Martinez-Zapater, 1985) or Nei's (Nei, 1972) genetic distances (Adam et al ., 1987 ;Glaszmann, 2 0 5 1987) . In recent studies, multivariate analysis of multilocus allozyme data has been found to show greater sensitivity and statistical significance (Yeh et al ., 1985(Yeh et al ., , 1986) as this has been observed to account for interactions among isozyme loci that are not observable using averaging techniques such as Nei's genetic distance .…”

Genetic Variability in Natural Populations of Eurytopic Ostracod Candona neglecta Sars

“…The statistical significance of differences in the allele frequency between populations was tested by means of a likelihoodratio test G 2 (Adam, 1987). Genetic identity (I) and genetic distance (D) between local populations were estimated after Nei (1972).…”