Allogeneic synovial membrane-derived mesenchymal stem cells do not significantly affect initial inflammatory parameters in a LPS-induced acute synovitis model

Rosa, Gustavo dos Santos; Krieck, André Massahiro Teramoto; Padula, E; Pfeifer, João Pedro Hübbe; Souza, Jaqueline Brandão de; Rossi, Mariana Correa; Stievani, Fernanda de Castro; Deffune, Elenice; Takahira, Regina Kiomi; Alves, Ana Liz Garcia

doi:10.1016/j.rvsc.2020.08.001

Cited by 1 publication

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“…Although allogeneic MSCs can modulate the immune system, the immune recognition can still occur in some cases, leading to cellular and humoral reactions against donor MSCs (Berglund and Schnabel, 2017). Also, despite exhibiting good results due to direct modulation of cell death in the recipient and to their paracrine action (Naji et al, 2019), MSCs efficacy can be affected by the inflammation, especially in acute cases (Rosa et al, 2020), and freely injected MSCs can also migrate to other tissues (Santos et al, 2019). In this sense, MSC encapsulation creates a physical barrier that does not allow crossing of cells or high molecular weight particles-like antibodies and complement factors-but allows the diffusion of nutrients, metabolites and oxygen at the same time (Orive et al, 2003;Hernández et al, 2010;Nafea et al, 2011).…”

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confidence: 99%

In Vitro Biological Performance of Alginate Hydrogel Capsules for Stem Cell Delivery

Souza

Rosa

Rossi

et al. 2021

Front. Bioeng. Biotechnol.

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Encapsulation of biological components in hydrogels is a well described method for controlled drug delivery of proteins, tissue engineering and intestinal colonization with beneficial bacteria. Given the potential of tissue engineering in clinical practice, this study aimed to evaluate the feasibility of encapsulation of adipose tissue-derived mesenchymal stem cells (MSCs) of mules in sodium alginate. We evaluated capsule morphology and cell viability, immunophenotype and release after encapsulation. Circular and irregular pores were observed on the hydrogel surface, in which MSCs were present and alive. Capsules demonstrated good capacity of absorption of liquid and cell viability was consistently high through the time points, indicating proper nutrient diffusion. Flow cytometry showed stability of stem cell surface markers, whereas immunohistochemistry revealed the expression of CD44 and absence of MHC-II through 7 days of culture. Stem cell encapsulation in sodium alginate hydrogel is a feasible technique that does not compromise cell viability and preserves their undifferentiated status, becoming a relevant option to further studies of tridimensional culture systems and in vivo bioactive agents delivery.

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