Allogeneic platelet rich plasma serves as a scaffold for articular cartilage derived chondroprogenitors

Vinod, Elizabeth; Francis, Deepak Vinod; Amirtham, Soosai Manickam; Sathishkumar, Solomon; Boopalan, P.R.J.V.C.

doi:10.1016/j.tice.2018.12.006

Cited by 19 publications

(22 citation statements)

References 32 publications

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“…For instance, PRP can stimulate dermal fibroblast migration and proliferation, showing its potential in wound treatment and skin rejuvenation [15]. Therefore, the incorporation of PRP in scaffolds is an advantageous approach, because it represents a simple, efficient and cost-effective method, allowing the immobilization of a number of highly-concentrated bioactive factors, creating an optimized micro-environment, and impacting upon tissue regeneration [10,16,17,18,19]. Various approaches have been developed to immobilize growth factors inside the scaffolds, and these may be envisioned for immobilizing PRP, such as physical encapsulation, absorption onto the biomaterial surface, layer-by-layer self-assembly, covalent conjunctions and extracellular matrix-based binding approaches [20].…”

Section: Introductionmentioning

confidence: 99%

The Use of Platelet-Rich Plasma to Promote Cell Recruitment into Low-Molecular-Weight Fucoidan-Functionalized Poly(Ester-Urea-Urethane) Scaffolds for Soft-Tissue Engineering

et al. 2019

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Due to their elastomeric behavior, polyurethane-based scaffolds can find various applications in soft-tissue engineering. However, their relatively inert surface has to be modified in order to improve cell colonization and control cell fate. The present study focuses on porous biodegradable scaffolds based on poly(ester-urea-urethane), functionalized concomitantly to the scaffold elaboration with low-molecular-weight (LMW) fucoidan; and their bio-activation with platelet rich plasma (PRP) formulations with the aim to promote cell response. The LMW fucoidan-functionalization was obtained in a very homogeneous way, and was stable after the scaffold sterilization and incubation in phosphate-buffered saline. Biomolecules from PRP readily penetrated into the functionalized scaffold, leading to a biological frame on the pore walls. Preliminary in vitro assays were assessed to demonstrate the improvement of scaffold behavior towards cell response. The scaffold bio-activation drastically improved cell migration. Moreover, cells interacted with all pore sides into the bio-activated scaffold forming cell bridges across pores. Our work brought out an easy and versatile way of developing functionalized and bio-activated elastomeric poly(ester-urea-urethane) scaffolds with a better cell response.

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Section: Introductionmentioning

confidence: 99%

The Use of Platelet-Rich Plasma to Promote Cell Recruitment into Low-Molecular-Weight Fucoidan-Functionalized Poly(Ester-Urea-Urethane) Scaffolds for Soft-Tissue Engineering

et al. 2019

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“…[2] Platelet-rich plasma could be a promising bioactive scaffold for the delivery of chondroprogenitors in cartilage healing due to its synergistic effect in supporting cell proliferation, maintaining cell viability and favoring extracellular matrix production. [3] The combination of intraarticular infiltrations with intraosseous infiltrations of PRP regulates the biological processes of the tissues, reducing the inflammatory environment and modulating the overexpression of biomolecules that generate an aberrant cellular behavior. Preliminary clinical results are promising, while further research and developing adequate protocols are necessary.…”

mentioning

confidence: 99%

Every new technique either conservative or surgical is good?

Atik¹

2019

Eklem Hastalik Cerrahisi

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“…35 Recently, many studies have used PRP alone or in combination with other scaffold materials in cartilage tissue engineering. [35][36][37][38] PRP is a carrier of multiple cytokines, PDGF, IGF-1, and FGF among them promoting the regeneration of chondrocytes and VEGF, TGF-1 and PDGF-BB promoting angiogenesis. 39,40 Additionally, PRP forms a 3D porous fibrin structure, allowing diffusion of the surrounding components and supporting the differentiation of chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

“…39,40 Additionally, PRP forms a 3D porous fibrin structure, allowing diffusion of the surrounding components and supporting the differentiation of chondrocytes. 38 By using PRP as a scaffold, Yanaga et al produced clinically applicable cartilage blocks. 10 Thus, we believe that PRP may be an ideal bioactive scaffold for cartilage tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

Creating Cartilage in Tissue-Engineered Chamber Using Platelet-Rich Plasma Without Cell Culture

Wang

Chen

et al. 2020

Tissue Engineering Part C: Methods

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Background: Clinically available cartilage, such as large-volume tissue-engineered cartilage, is urgently required for various clinical applications. Tissue engineering chamber (TEC) models are a promising organlevel strategy for efficient enlargement of cells or tissues within the chamber. The conventional TEC technology is not suitable for cartilage culture, because it lacks the necessary chondrogenic growth factor, which is present in platelet-rich plasma (PRP). In this study, we added autogenous auricular cartilage fragments mixed with PRP in a TEC to obtain a large amount of engineered cartilage. Experiment: To prove the efficacy of this method, 48 New Zealand white rabbits were randomly divided into 4 groups: PRP, vascularized (Ves), PRP, PRP+Ves, and control. Auricular cartilage was harvested from the rabbits, cut into fragments (2 mm), and then injected into TECs. Cartilage constructs were harvested at week 8, and construct volumes were measured. Histological morphology, immunochemical staining, and mechanical strength were evaluated. Results: At week 8, PRP+Ves constructs developed a white, cartilage-like appearance. The volume of cartilage increased by 600% the original volume from 0.30 to 1.8-0.1789 mL. Histological staining showed proliferation of edge chondrocytes in the embedded cartilage in the PRP and PRP+Ves groups. Furthermore, the cartilage constructs in the PRP+Ves group show mechanical characteristics similar to those of normal cartilage. Conclusions: Auricular cartilage fragments mixed with PRP and vascularization of the TEC showed a significantly increased cartilage tissue volume after 8 weeks of incubation in rabbits.

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Allogeneic platelet rich plasma serves as a scaffold for articular cartilage derived chondroprogenitors

Cited by 19 publications

References 32 publications

The Use of Platelet-Rich Plasma to Promote Cell Recruitment into Low-Molecular-Weight Fucoidan-Functionalized Poly(Ester-Urea-Urethane) Scaffolds for Soft-Tissue Engineering

The Use of Platelet-Rich Plasma to Promote Cell Recruitment into Low-Molecular-Weight Fucoidan-Functionalized Poly(Ester-Urea-Urethane) Scaffolds for Soft-Tissue Engineering

Every new technique either conservative or surgical is good?

Creating Cartilage in Tissue-Engineered Chamber Using Platelet-Rich Plasma Without Cell Culture

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