Allium�hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2‑related factors in an ovalbumin‑induced asthma mouse model

Abstract

In 2013, WHO estimated that approximately 235 million people suffered from asthma worldwide. Asthma is a hyper responsive disorder, which is related to an imbalance between the T-helper type 1 and 2 cells (henceforth, Th1 and Th2, respectively). Allium hookeri is a plant that is widely used for culinary purposes and also in traditional Asian medicine. The present study was conducted to elucidate the anti-asthmatic effects and mechanism of action of A. hookeri root extracts (AHRE) in an ovalbumin (OVA)-induced asthma mouse model. The mice were divided into five groups, namely, the control, the OVA-treated group, the dexamethasone-treated group, the 30 mg/kg AHRE-treated group, and the 300 mg/kg AHRE-treated group. The total WBC count and the differential cell count in the bronchoalveolar fluid, the level of serum IgE, the histopathological changes in the lung, and changes in the cell surface molecules, the asthma-related cytokine levels, and Th cell transcription factors were evaluated. AHRE significantly ameliorated asthmatic changes, such as the total WBC count, eosinophil count, and the level of IgE; in addition, it reduced mucus hypersecretion, epithelial hyperplasia, and eosinophil infiltration in the lungs. AHRE significantly inhibited the expression of CD68+ cells and MHC class II+ molecules, Th1 cell transcription factor (T-bet) activation, Th2 cell transcription factor (GATA-3) activation, and TNF-α in the lung tissue. Furthermore, it suppressed cell surface molecules, such as CD4+and CD8+; Th1-related cytokines, such as IFN-γ and IL-12p40; Th2-related cytokines, such as IL-4 and IL-5; and Th17-related cytokines, such as IL-6 and TNF-α, in a dose-dependent manner. Thus, AHRE may be considered a promising anti-asthmatic drug.

The mediatory role of Majie cataplasm on inflammation of allergic asthma through transcription factors related to Th1 and Th2

“…And plenty of literature has shown that T lymphocyte retains influence on asthma. The mainstream view is that the pathogenesis of asthma is the imbalance of T cells differentiation (predominantly Th1/Th2), and various pro-inflammatory factors may greatly assist in Th2 inflammation [4].…”

Codonopsis laceolata Water Extract Ameliorates Asthma Severity by Inducing Th2 Cells’ and Pulmonary Epithelial Cells’ Apoptosis via NF-κB/COX-2 Pathway

“…Anti-IgE agents, such as omalizumab, are immunomodulatory and are called the 'magic bullet' for allergic asthma treatment as they can suppress the exacerbation of asthma [39]. In our lab, we have been concentrating on finding Th2 cell-suppressing agents from natural products [13,[40][41][42].…”

Drug Development from Natural Products Based on the Pathogenic Mechanism of Asthma

“…Allium cepa L., which has long been used as an anti-inflammatory drug, downregulates Blomia tropicalis-induced Th2 cell-related cytokines, such as IL-4 and IL-13 [83]. Allium hookeri, which is used as a culinary material, significantly inhibits the release of Th2 cell-related cytokines, such as IL-4, IL-5, and IL-13 [84]. Anoectochilus formosanus HAYATA, a traditional drug, suppressed IgE, IL-4, and IL-5 levels in an asthma model [85].…”