The mediatory role of Majie cataplasm on inflammation of allergic asthma through transcription factors related to Th1 and Th2

Ji, Wenting; Shi, Hanfen; Dong, Ruijuan; Ge, Dongyu; Du, Xin; Ren, Beida; Wang, Xueqian; Wang, Qingguo

doi:10.1186/s13020-020-00334-w

Cited by 10 publications

(9 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Adaptive lymphocytes, therefore, has clearly added a selective advantage compared to that of ILCs in conferring protective immunity under natural conditions. In our previous study, Majie cataplasm could reduce the increasing number of Th1 and Th2 in the asthma model group, which meant that the adaptive cells supported the leading role in asthma inflammation [ 20 ]. And in order to confirm this, we detected other adaptive cells, Treg and B10s.…”

Section: Discussionmentioning

confidence: 99%

“…For a mouse, the area is 0.2 cm 2 . The production flow chart of Majie cataplasm was referred to our previous study [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…According to the previous experiment [ 20 , 21 ], C57 mice ( n = 40) were divided into 4 groups, including the control group ( n = 10), the asthma model group ( n = 10), the dexamethasone group ( n = 10), and the Majie cataplasm group ( n = 10).…”

Section: Methodsmentioning

confidence: 99%

“…On the fifteenth day, mice in the control group and the asthma model group were given no intervention, mice in the dexamethasone group were injected intraperitoneally with the dexamethasone sodium phosphate injection 0.1 ml/mouse (2 mg/kg) [ 22 , 23 ], and mice in the Majie cataplasm group were patched with cataplasm on the mice's backs, each cataplasm staying for 24 hours [ 20 ]. After 10 days, all the mice were euthanized by cervical dislocation for the following measurements.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Majie Cataplasm Promotes Th1 Response to Fight against Asthmatic Th2 Inflammation through NKs

Shi

Feng

et al. 2022

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

Background. Immune cells are tightly bound up with the pathogenesis of asthma. Besides T cells, B cells, macrophages, and mast cells, the mechanism of innate lymphoid cells (ILCs) in asthma is gradually explicit. As a kind of traditional Chinese medicine, Majie cataplasm realizes its potential in the clinical setting as an adjuvant for asthma. In our previous experiments, Majie cataplasm inhibits the increasing Th1 and Th2 in allergic asthma inflammation and reshapes a balance between Th1 and Th2. As ILCs are the reflection of Th cells in lung tissues, we will figure out whether Majie cataplasm could have similar effects on ILCs or not. Methods. A total of 40 female C57/BL6 mice were randomly divided into the control group (n = 10), the asthma model group (n = 10), the dexamethasone group (n = 10), and the Majie cataplasm group (n = 10). Except for the control group, mice were sensitized with ovalbumin (OVA) and excited to establish mice models of asthma. Lung tissue and splenic tissue were collected at 24 h after the last challenge with OVA, and the cell suspension of the lungs and spleen was prepared. The number of ILC1s, ILC2s, ILC3s, and NKs cells in the lungs and Tregs and B10s in the spleen were detected by flow cytometry (FCM). This was followed by simultaneous quantitative detection of 40 inflammatory cytokines and chemokines in the lung by a protein microarray. Results. The dexamethasone and Majie cataplasm could restore the number of ILC1s, ILC2s, and ILC3s in lung tissue. Compared with the control group, these cells remained unchanged in the asthma model group, while ILC1s ( P < 0.001 , P < 0.01 ), ILC2s ( P < 0.001 , P < 0.01 ), and ILC3s ( P < 0.01 , P < 0.05 ) were restored after the intervention of dexamethasone and Majie cataplasm. The number of NKs was low among the control group, the asthma model group, and the dexamethasone group, while the number of NKs rocketed in the Majie cataplasm group ( P < 0.0001 ). For splenic Tregs and B10s, Majie cataplasm could curb the increasing numbers of them in the asthma model group ( P < 0.0001 , P < 0.01 ), while only Tregs were suppressed by the dexamethasone ( P < 0.0001 ). For the inflammatory cytokines in the lung, the contents of TNF-α, TNFR2, CXCL-9, CCL-12, CCL-9, CCL-2, and CCL-5 in the asthma model group were higher than those in the control group, while the contents of GM-CSF and IL-1α were decreased. Comparing the asthma model group to the dexamethasone group, the levels of G-CSF, CCL-9, CCL-5, and TNFR2 in the former group were higher. The levels of TNF-α, TNFR2, and CCL-9 in the asthma model group increase, while the levels of IFN-γ, IL-1α, ICAM-1, and IL-4 increased in the Majie cataplasm group, especially IFN-γ and IL-1α. Conclusion. Both the dexamethasone and Majie cataplasm could control the asthmatic inflammation by reducing the inflammatory factors, inhibiting the adaptive inflammation reaction in the latter stage of inflammation and furtherly reversing the inhibition of ILC2s, ILC2s, and ILC3s. In addition, Majie cataplasm can promote the quantity of NKs and the content of IL-1α and IFN-γ, induce IFN-γ+NKs to shut down the Th2 response, and tend to elicit the Th1 response.

show abstract

Section: Discussionmentioning

confidence: 99%

“…For a mouse, the area is 0.2 cm 2 . The production flow chart of Majie cataplasm was referred to our previous study [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Majie Cataplasm Promotes Th1 Response to Fight against Asthmatic Th2 Inflammation through NKs

Shi

Feng

et al. 2022

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, it was observed in a murine asthma model that treatment with a novel H 4 R antagonist (N-(2-Aminoethyl)-5-chloro-1H-indole-2-carboxamide) decreased the activation of ERK1/2, Akt, SAPK/JNK and NF-κB signaling pathways, in addition to reduced Th2 cytokines production in BAL and eosinophilic infiltration in lung tissue [14]. Activation of the STAT3 signaling pathway proved to be important for Th2 cells differentiation, cytokines production, allergic inflammatory response increasing and airway remodeling [53,54]. Studies showed that inhibition of the STAT3 signaling pathway in mast cells impaired FcεRI-mediated signaling and reduced degranulation in humans [55].…”

Section: Discussionmentioning

confidence: 99%

The Novel H4R Antagonist 1-[(5-Chloro-2,3-Dihydro-1-Benzofuran-2-Yl)Methyl]-4-Methyl-Piperazine (LINS01007) Attenuates Several Symptoms in Murine Allergic Asthma

Balbino

Lima

Fernandes

et al. 2020

Cell Physiol Biochem

View full text Add to dashboard Cite

BACKGROUND/AIMS: Histamine is an important chemical transmitter involved in inflammatory processes, including asthma and other chronic inflammatory diseases. Its inflammatory effects involve mainly the histamine H4 receptor (H4R), whose role in several studies has already been demonstrated. Our group have explored the effects of 1-[(2,3-dihydro-1-benzofuran-2-yl)methyl]piperazines as antagonists of H4R, and herein the compounds LINS01005 and LINS01007 were studied with more details, considering the different affinity profile on H4R and the anti-inflammatory potential of both compounds. METHODS: We carried out a more focused evaluation of the modulatory effects of LINS01005 and LINS01007 in a murine asthma model. The compounds were given i.p. (1-7 mg/kg) to ovalbumin sensitized BALB/c male mice (12 weeks old) 30 min before the antigen challenging, and after 24 h the cell analysis from the bronchoalveolar lavage fluid (BALF) was performed. The lung tissue was used for evaluation by western blot (COX-2, 5-LO, NF-κB and STAT3 expressions) and histological analysis. RESULTS: Treatment with the more potent H4R antagonist LINS01007 significantly decreased the total cell count and eosinophils in BALF at lower doses when compared to LINS01005. The expression of COX-2, 5-LO, NF-κB and STAT3 in lung tissue was significantly reduced after treatment with LINS01007. Morphophysiological changes such as mucus and collagen production and airway wall thickening were significantly reduced after treatment with LINS01007. CONCLUSION: These results show important down regulatory effect of novel H4R antagonist (LINS01007) on allergic lung inflammation.

show abstract

Transdermal Delivery of Chinese Medicine

Yin¹,

Dong²,

Dong³

et al. 2021

Novel Drug Delivery Systems for Chinese Medicines

View full text Add to dashboard Cite

The mediatory role of Majie cataplasm on inflammation of allergic asthma through transcription factors related to Th1 and Th2

Cited by 10 publications

References 48 publications

Majie Cataplasm Promotes Th1 Response to Fight against Asthmatic Th2 Inflammation through NKs

Majie Cataplasm Promotes Th1 Response to Fight against Asthmatic Th2 Inflammation through NKs

The Novel H4R Antagonist 1-[(5-Chloro-2,3-Dihydro-1-Benzofuran-2-Yl)Methyl]-4-Methyl-Piperazine (LINS01007) Attenuates Several Symptoms in Murine Allergic Asthma

Transdermal Delivery of Chinese Medicine

Contact Info

Product

Resources

About