Allicin enhances chemotherapeutic response and ameliorates tamoxifen-induced liver injury in experimental animals

Abstract

Context: Tamoxifen (TAM) is widely used for treatment of hormone-dependent breast cancer; however, it may be accompanied with hepatic injury. Allicin is the most abundant thiosulfinate molecule from garlic with the potential to provide beneficial effects on various diseases. Objective: To elucidate the effect of commercially available allicin on both antitumor activity and liver injury of TAM. Materials and methods: The cytotoxicity of TAM and/or allicin was evaluated in vitro using cultured Ehrlich ascites carcinoma (EAC) cells and in vivo against murine tumor (solid) model of EAC. TAM induced liver injury in rats by intraperitoneally (i.p.) injection at a dose of 45 mg/kg, for 7 successive days.Results: TAM at a dose of 3 mM (IC 50 ) significantly decreased percent survival of EAC to 52%. TAM combination with allicin (5 or 10 mM) showed a significant cytotoxic effect compared with the TAM-treated group as manifested by a decrease in percent survival of EAC to 35% and 29%, respectively. Allicin (10 mg/kg, orally) enhanced the efficacy of TAM (1 mg/kg, i.p.) in mice as manifested by a significant increase in solid tumor growth inhibition by 82% compared with 70% in the TAM group. In rats, TAM intoxication resulted in a significant decline in SOD, GSH, and total protein with significant elevation in TBARS, ALT and AST, ALP, LDH, total bilirubin, gGT, and TNF-a levels. These changes are abrogated by allicin treatment. Discussion and conclusion: The results suggest the beneficial role of allicin as an adjuvant to TAM in cancer treatment by alleviating liver injury.

Study theeffect ofTamoxifenon Lipid profilein Male Albino Rats

“…In view of the results of the current study there is a significant increase in the level of serum Lipids when treated with TAM and elevation increased as the dose multiplied increased by the level of (30, 40, 50)mg/kg compared to the control group and this is consistent with [10][11][12][13].…”

Toxicity of anticancer drugs and its prevention with special reference to role of garlic constituents

“…However, SAMC was shown to markedly suppress the cisplatin-induced elevation in the level of inflammation cytokines including TNF-, IL-1, TGF1, COX-2 and iNOS clearly indicating that SAMC exerted an anti-inflammatory effect in cisplatin-induced renal injury as well as hepatotoxicity in rat model (Xiao et al, 2013;Zhu et al, 2017). Suddek, 2014Zheng et al, 1997Miron et al, 2003;Zhang et al, 2010;Chu et al, 2012;Wang et al, 2012;Lee et al, 2013 Suddek, 2014Zheng et al, 1997Miron et al, 2003;Zhang et al, 2010;Chu et al, 2012;Wang et al, 2012;Lee et al, 2013 162.26 Diallyl thiosulfinate (Allicin) 2. Siegers et al,1999 161.22 Alliin 1.…”

“…A study of Suddek (2014) revealed that the pre-treatment with allicin potentiates the antitumor effect of tamoxifen and protects animals against hepatic injury by preventing oxidative stress and lipid peroxidation, enhancing antioxidant enzyme activities and inhibiting hepatic inflammation.…”

Allium sativum

“…Mice treated with 250 μg of ajoene had only 4.9 % the number of tumours per mouse compared with the control group at 18 weeks. Tamoxifen (TAM) combination with allicin (5 or 10 μM) showed a signifi cant cytotoxic effect compared with the TAM-treated group as manifested by a decrease in the percent survival of Ehrlich ascites carcinoma to 35 % and 29 %, respectively (Suddek 2014 ). Allicin (10 mg/kg, orally) enhanced the effi cacy of TAM (1 mg/kg, i.p.)…”