Alleviation of Synovial Inflammation of Juanbi-Tang on Collagen-Induced Arthritis and TNF-Tg Mice Model

Abstract

Rheumatoid arthritis (RA) is a chronic autoimmune disease that is primarily characterized by synovial inflammation. In this study, we found that a traditional Chinese decoction, Juanbi-Tang (JBT), JBT attenuated the symptoms of collageninduced arthritis (CIA) mice and in tumor necrosis factor transgenic (TNF-Tg) mice by attenuating the arthritis index and hind paw thickness. According to histopathological staining of ankle sections, JBT significantly decreased the area of inflammation and reduced bone destruction of ankle joints in both these two types of mice. Moreover, decreased tartaric acid phosphatase-positive osteoclasts were observed in the JBT group compared with those found in the control group. We also revealed that JBT suppressed monocytes and T cells as well as the production of CCL2, CCR6, and CXCR3 ligands. We next used high-performance liquid chromatography to investigate the components and pharmacological properties of this classical herbal medicine in traditional Chinese medicine. Based on network pharmacology, we performed computational prediction simulation of the potential targets of JBT, which indicated the NF-kappa B pathway as its target, which was confirmed in vitro. JBT suppressed the production of pro-inflammatory cytokines including interleukin-6 (IL-6) and IL-8, and inhibited the expression of matrix metalloproteinase 1 in fibroblast-like synoviocytes derived from RA patients (MH7A cells). Furthermore, JBT also suppressed the phosphorylation of p38, JNK, and p65 in TNF-a-treated MH7A cells. In summary, this study proved that JBT could inhibit synovial inflammation and bone destruction, possibly by blocking the phosphorylation of NF-kappa B pathway-mediated production of proinflammatory effectors.

Assessing the Efficacy of Chinese Herbal Medicine for Dialysis-related Myofascial Pain in the Fistula Arm: Study Protocol for a Pilot Randomized Trial

“…Signi cant reductions in the serum levels of MMP-2 and MMP-9 indicate that JBT has a role to play in preventing osteoarthritis [41]. Similar effects of JBT on synovial in ammation and bone destruction through inhibiting the pro-in ammatory cytokines have been recently reported by Wang et al [42]. In addition, a study by Zhao et al has demonstrated that JBT ameliorates bone destruction and reduces bone loss induced by rheumatoid arthritis [43].…”

“…At present, the ndings of most animal studies have con rmed the bene ts of JBT in improving arthritis and synovitis [41][42][43]. Signi cant reductions in the serum levels of MMP-2 and MMP-9 indicate that JBT has a role to play in preventing osteoarthritis [41].…”

Assessing the efficacy and safety of Juan Bi Tang for dialysis-related myofascial pain in the fistula arm: Study protocol for a randomized cross-over trial

“…Significant reductions in the serum levels of MMP-2 and MMP-9 indicate that JBT has a role to play in preventing osteoarthritis (45). Similar effects of JBT on synovial inflammation and bone destruction through inhibiting the proinflammatory cytokines have recently been reported by Wang et al (46). In addition, a study by Zhao et al has demonstrated that JBT ameliorates bone destruction and reduces bone loss induced by rheumatoid arthritis (47).…”

“…At present, the findings of most animal studies have confirmed the benefits of JBT in improving arthritis and synovitis (45)(46)(47). Significant reductions in the serum levels of MMP-2 and MMP-9 indicate that JBT has a role to play in preventing osteoarthritis (45).…”

Low-Dose Interleukin-2 Altered Gut Microbiota and Ameliorated Collagen-Induced Arthritis

“…The arthritis index (AI) 35 , 36 for disease severity was determined based on the method in a previous report. The mice were examined three times per week.…”