Alleviation of cold damage by exogenous application of melatonin in vegetatively propagated tea plant ( Camellia sinensis (L.) O. Kuntze)

Li, Jiahao; Arkorful, Emmanuel; Cheng, Siyuan; Zhou, Qi; Li, Huan; Chen, Xuan; Sun, Kang; Li, Xinghui

doi:10.1016/j.scienta.2018.04.068

Cited by 54 publications

(25 citation statements)

References 63 publications

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“…There were different concentrations applied to different plant species and organs. Our previous study indicated that 100 μM melatonin was the most effective concentration for tea leaves [40]. In the present study, we analyzed the effects of 100 μM melatonin on tea plants under abiotic stress.…”

Section: Discussionmentioning

confidence: 95%

Exogenous Melatonin Enhances Cold, Salt and Drought Stress Tolerance by Improving Antioxidant Defense in Tea Plant (Camellia sinensis (L.) O. Kuntze)

et al. 2019

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Melatonin is a biological hormone that plays crucial roles in stress tolerance. In this study, we investigated the effect of exogenous melatonin on abiotic stress in the tea plant. Under cold, salt and drought stress, increasing malondialdehyde levels and decreasing maximum photochemical efficiency of PSII were observed in tea leaves. Meanwhile, the levels of reactive oxygen species (ROS) increased significantly under abiotic stress. Interestingly, pretreatment with melatonin on leaves alleviated ROS burst, decreased malondialdehyde levels and maintain high photosynthetic efficiency. Moreover, 100 μM melatonin-pretreated tea plants showed high levels of glutathione and ascorbic acid and increased the activities of superoxide dismutase, peroxidase, catalase and ascorbate peroxidase under abiotic stress. Notably, melatonin treatments can positively up-regulate the genes (CsSOD, CsPOD, CsCAT and CsAPX) expression of antioxidant enzyme biosynthesis. Taken together, our results confirmed that melatonin protects tea plants against abiotic stress-induced damages through detoxifying ROS and regulating antioxidant systems.

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Section: Discussionmentioning

confidence: 95%

Exogenous Melatonin Enhances Cold, Salt and Drought Stress Tolerance by Improving Antioxidant Defense in Tea Plant (Camellia sinensis (L.) O. Kuntze)

et al. 2019

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“…For the POD activity analysis, 0.1 g sample was ground using liquid nitrogen in 0.9 cm 3 of 0.9 % (m/v) NaCl, then extract was centrifuged at 12 000 g for 10 min. The supernatant was used to determine the POD activity by the imaging reader (Cytation3, BioTek, Winooski, VT, USA) in Nanjing Jiancheng Bioengineering Institute, China (Li et al 2018). One unit (U) was defined as the change of absorbance at 470 nm of 0.01 per min.…”

Section: Assay Of Pod Activitymentioning

confidence: 99%

Isolation and expression profiles of class III PRX gene family under drought stress in Camellia sinensis

Li¹,

Wang²,

Chen³

et al. 2020

Biologia plant.

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The class III PRX family is a class of heme-containing oxidases and plays important roles in response to abiotic stress in plants. The responses to abiotic stresses could be regulated by phytohormones like abscisic acid (ABA) and methyl jasmonate (MeJA). In this research, 11 CsPRXs genes in tea plant (Camellia sinensis) were cloned and analyzed. Based on the similarity of the sequences, they were classified into 5 sub-groups. According to the results of reverse transcription PCR, CsPRX55 presented the highest expression in roots compared to stems and leaves of both tea cultivars LJ43 and Baiye 1. Besides, the expressions of CsPRX12 and CsPRX73 were highest in roots, while CsPRX4, CsPRX47, and CsPRX72 were highest in stems of 'Baiye 1'. But most of CsPRXs showed the highest expressions in leaves of 'LJ43'. CsPRXs appeared different expression patterns under drought stress of tea plants which were pre-treated with ABA or MeJA for three days. In 'LJ43' and 'Baiye 1', there were 3 CsPRXs and 7 CsPRXs up-regulated by exogenous ABA and MeJA, respectively. However, CsPRX4 and CsPRX16 were down-regulated in 'LJ43' treated with ABA and MeJA. It suggested that CsPRXs possessed diverse functions in response to hormones and abiotic stress. In 'LJ43', the activity of peroxidase (POD) was increased when pre-treated by ABA and MeJA, and the highest activity appeared after 24 and 12 h, respectively. In 'Baiye 1', the activity of POD was also increased, however, when pre-treated by MeJA, the peak-time of POD activity was at 24 h. But the change had no obvious rule after ABA treatment. Exogenous ABA or MeJA may play a role in protecting tea plants suffered from drought stress via regulating some CsPRXs expressions and increasing POD activity.

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“…Melatonin has also been shown to scavenge ROS efficiently in vivo using transgenic plants, leading to oxidative stress resistance ( Park et al, 2013 ). Exogenous melatonin increases the activity of superoxide dismutase (SOD; EC 1.15.1.1), peroxidase (POD; EC 1.11.1.7), catalase (CAT; EC 1.11.1.6), and ascorbate peroxidase (APX; EC 1.11.1.11), thus enhancing the antioxidant capacity and reducing the effects of cold and water stress on tea trees ( Li et al, 2018a ) and cucumber seedlings ( Zhang et al, 2013 ). Ascorbic acid (AsA) and glutathione (GSH) are important reducing substances in plants, where they provide defense against membrane lipid peroxidation.…”

Section: Introductionmentioning

confidence: 99%

Exogenous melatonin improves salt stress adaptation of cotton seedlings by regulating active oxygen metabolism

Jiang

Liu

et al. 2020

PeerJ

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Melatonin is a small-molecule indole hormone that plays an important role in participating in biotic and abiotic stress resistance. Melatonin has been confirmed to promote the normal development of plants under adversity stress by mediating physiological regulation mechanisms. However, the mechanisms by which exogenous melatonin mediates salt tolerance via regulation of antioxidant activity and osmosis in cotton seedlings remain largely unknown. In this study, the regulatory effects of melatonin on reactive oxygen species (ROS), the antioxidant system, and osmotic modulators of cotton seedlings were determined under 0–500 µM melatonin treatments with salt stress induced by 150 mM NaCl treatment. Cotton seedlings under salt stress exhibited an inhibition of growth, excessive hydrogen peroxide (H2O2), superoxide anion (O2−), and malondialdehyde (MDA) accumulations in leaves, increased activity levels of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and elevated ascorbic acid (AsA) and glutathione (GSH) content in leaves. However, the content of osmotic regulators (i.e., soluble sugars and proteins) in leaves was reduced under salt stress. This indicates high levels of ROS were produced, and the cell membrane was damaged. Additionally, osmotic regulatory substance content was reduced, resulting in osmotic stress, which seriously affected cotton seedling growth under salt stress. However, exogenous melatonin at different concentrations reduced the contents of H2O2, O2−, and MDA in cotton leaves, increased the activity of antioxidant enzymes and the content of reductive substances (i.e., AsA and GSH), and promoted the accumulation of osmotic regulatory substances in leaves under salt stress. These results suggest that melatonin can inhibit ROS production in cotton seedlings, improve the activity of the antioxidant enzyme system, raise the content of osmotic regulation substances, reduce the level of membrane lipid peroxidation, and protect the integrity of the lipid membrane under salt stress, which reduces damage caused by salt stress to seedlings and effectively enhances inhibition of salt stress on cotton seedling growth. These results indicate that 200 µM melatonin treatment has the best effect on the growth and salt tolerance of cotton seedlings.

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Alleviation of cold damage by exogenous application of melatonin in vegetatively propagated tea plant ( Camellia sinensis (L.) O. Kuntze)

Cited by 54 publications

References 63 publications

Exogenous Melatonin Enhances Cold, Salt and Drought Stress Tolerance by Improving Antioxidant Defense in Tea Plant (Camellia sinensis (L.) O. Kuntze)

Exogenous Melatonin Enhances Cold, Salt and Drought Stress Tolerance by Improving Antioxidant Defense in Tea Plant (Camellia sinensis (L.) O. Kuntze)

Isolation and expression profiles of class III PRX gene family under drought stress in Camellia sinensis

Exogenous melatonin improves salt stress adaptation of cotton seedlings by regulating active oxygen metabolism

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