Alleviating chronic kidney disease progression through modulating the critical genus of gut microbiota in a cisplatin-induced Lanyu pig model

Lee, Ya-Jane; Li, Kuanyi; Wang, Pin-Jhu; Huang, Hung‐Chang; Chen, Ming‐Ju

doi:10.1016/j.jfda.2019.10.001

Cited by 22 publications

(19 citation statements)

References 51 publications

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“…In addition, the relative abundance of three members of the phylum Firmicutes (Clostridium_sensu_stricto_1, Ruminococcaceae_UCG-014, and Clostridium_sensu_stricto_6) and norank_o_Mollicutes_RF9 exhibited significantly lower levels (P < 0.01, P < 0.05, respectively) at 21 dpi in the PCV3inoculated piglets compared to that in sham-inoculated piglets, which reduced the ratio of Firmicutes to Bacteroidetes phyla and finally weakened the capacity to obtain energy from diet, thereby, leading to weight loss (Ley et al, 2006;Henning et al, 2019;Jiang et al, 2019;Li et al, 2019). Moreover, Blantia, Coprococcus, and unclassified_f_Lachnospiraceae displayed positive anti-inflammatory effects, resulting in the weakening of inflammatory responses (Jenq et al, 2015;Di Iorio et al, 2019;Lee et al, 2020) and exhibiting a marked increase in PCV3-inoculated piglets at 28 dpi compared to those in shaminoculated piglets (P < 0.01, P < 0.05). These results explain the downregulation of inflammatory cytokines, such as TNF-α or IL-6, in the later stage of PCV3 infection (Jiang et al, 2019).…”

Section: Discussionmentioning

confidence: 96%

Dynamic Alterations of Gut Microbiota in Porcine Circovirus Type 3-Infected Piglets

et al. 2020

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Porcine circovirus type 3 (PCV3) is a novel porcine circovirus species associated with several diseases such as porcine dermatitis and nephropathy syndrome (PDNS)-like clinical signs, reproductive failure, cardiac pathologies, and multisystemic inflammation in piglets and sows. Currently, many studies have focused on the interaction between microbiota composition and disease progression. However, dynamic changes in the composition of the gut microbiota following PCV3 infection are still unknown. In this study, alterations in gut microbiota in PCV3-inoculated and sham-inoculated piglets were analyzed at various time points [7, 14, 21, and 28 days post-inoculation (dpi)] using the Illumina MiSeq platform. Using principal coordinate analysis, obvious structural segregations were observed in bacterial diversity and richness between PCV3-and sham-inoculated piglets, as well as at the four different time points. The abundance of gut microbiota exhibited a remarkable time-related decrease in Clostridium_sensu_stricto_1 in PCV3-inoculated piglets. In addition, significant differences were observed in functional classification based on cluster of orthologous groups assignment, between PCV3-and sham-inoculated piglets. Our findings demonstrated that PCV3 infection caused dynamic changes in the gut microbiota community. Therefore, regulating gut microbiota community may be an effective approach for preventing PCV3 infection.

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Section: Discussionmentioning

confidence: 96%

Dynamic Alterations of Gut Microbiota in Porcine Circovirus Type 3-Infected Piglets

et al. 2020

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“…Fecal acetate, propionate, and butyrate were determined with gas chromatography. Fecal samples were extracted using methyl ether with 4-methyl-n-valeric acid (Sigma) as an internal standard according to the method described previously [27]. The extracts were dissolved in 1 M of phosphoric acid before being injected into the gas chromatograph (GC-2014; Shimadzu Corp., Kyoto, Japan) fitted with a glass capillary column (0.25 mm × 30 m, Stabilwax-DA, Restek Corp., Bellefonte, PA, USA) and a flame ionization detector.…”

Section: Short-chain Fatty Acid Measurementmentioning

confidence: 99%

Administration of Lactobacillus reuteri Combined with Clostridium butyricum Attenuates Cisplatin-Induced Renal Damage by Gut Microbiota Reconstitution, Increasing Butyric Acid Production, and Suppressing Renal Inflammation

et al. 2021

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Cisplatin-induced nephrotoxicity is associated with gut microbiota disturbance. The present study aimed to investigate whether supplementation of Lactobacillus reuteri and Clostridium butyricum (LCs) had a protective effect on cisplatin-induced nephrotoxicity through reconstruction of gut microbiota. Wistar rats were given different treatments: control, cisplatin (Cis), cisplatin + C. butyricum and L. reuteri (Cis+LCs), and C. butyricum and L. reuteri (LCs). We observed that cisplatin-treated rats supplemented with LCs exhibited significantly decreased renal inflammation (KIM-1, F4/80, and MPO), oxidative stress, fibrosis (collagen IV, fibronectin, and a-SMA), apoptosis, concentration of blood endotoxin and indoxyl sulfate, and increased fecal butyric acid production compared with those without supplementation. In addition, LCs improved the cisplatin-induced microbiome dysbiosis by maintaining a healthy gut microbiota structure and diversity; depleting Escherichia-Shigella and the Enterobacteriaceae family; and enriching probiotic Bifidobacterium, Ruminococcaceae, Ruminiclostridium_9, and Oscillibacter. Moreover, the LCs intervention alleviated the cisplatin-induced intestinal epithelial barrier impairment. This study indicated LCs probiotic serves as a mediator of the gut–kidney axis in cisplatin-induced nephrotoxicity to restore the intestinal microbiota composition, thereby suppressing uremic toxin production and enhancing butyrate production. Furthermore, the renoprotective effect of LCs is partially mediated by increasing the anti-inflammatory effects and maintaining the integrity of the intestinal barrier.

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“…Typically, Lactobacillus salivarius BP121 suppresses the generation of uremic toxins and regulates AMPK- and TLR4-dependent TJ assembly, thus protecting against cisplatin-induced AKI by downregulating renal inflammatory mediators and decreasing the oxidative stress [ 71 ]. Lactobacillus mix addition also decreases the production of proinflammatory cytokines (TNF-α and IL-6) and prevents renal proximal tubular cell apoptosis in a cisplatin-induced renal injury in pig model [ 72 ]. Moreover, Bifidobacteria is another widely used probiotic along with Lactobacilli in kidney diseases [ 73 , 74 ].…”

Section: Therapies Targeting the Intestinal Microbiota In Akimentioning

confidence: 99%

Intestinal microbiota dysbiosis in acute kidney injury: novel insights into mechanisms and promising therapeutic strategies

et al. 2022

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In recent years, the clinical impact of intestinal microbiota–kidney interaction has been emerging. Experimental evidence highlighted a bidirectional evolutionary correlation between intestinal microbiota and kidney diseases. Nonetheless, acute kidney injury (AKI) is still a global public health concern associated with high morbidity, mortality, healthcare costs, and limited efficient therapy. Several studies on the intestinal microbiome have improved the knowledge and treatment of AKI. Therefore, the present review outlines the concept of the gut–kidney axis and data about intestinal microbiota dysbiosis in AKI to improve the understanding of the mechanisms of the intestinal microbiome on the modification of kidney function and response to kidney injury. We also introduced the future directions and research areas, emphasizing the intervention approaches and recent research advances of intestinal microbiota dysbiosis during AKI, thereby providing a new perspective for future clinical trials.

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Alleviating chronic kidney disease progression through modulating the critical genus of gut microbiota in a cisplatin-induced Lanyu pig model

Cited by 22 publications

References 51 publications

Dynamic Alterations of Gut Microbiota in Porcine Circovirus Type 3-Infected Piglets

Dynamic Alterations of Gut Microbiota in Porcine Circovirus Type 3-Infected Piglets

Administration of Lactobacillus reuteri Combined with Clostridium butyricum Attenuates Cisplatin-Induced Renal Damage by Gut Microbiota Reconstitution, Increasing Butyric Acid Production, and Suppressing Renal Inflammation

Intestinal microbiota dysbiosis in acute kidney injury: novel insights into mechanisms and promising therapeutic strategies

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