Alleviating acute alcoholic liver injury in mice with Bacillus subtilis co-expressing alcohol dehydrogenase and acetaldehyde dehydrogenase

Lü, Jing; Lyu, Yunbin; Li, Mingtong; Sun, Jing; Huang, Zhenghua; Lü, Fang; Lu, Zhaoxin

doi:10.1016/j.jff.2018.09.006

Cited by 25 publications

(3 citation statements)

References 42 publications

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“…ALDH2 in the mitochondria of liver cells is a crucial alcohol-metabolizing enzyme with substantial activity, capable of eliminating acetaldehyde, an intermediate product in alcohol metabolism associated with cancer and inflammation. Ethanol is extensively oxidized to acetaldehyde by ADH4 after ingestion, and then ALDH2 metabolizes acetaldehyde to acetic acid [39]. Meanwhile, the CYP2E1 metabolic pathway of ethanol amplifies the generation of ROS, such as hydroxyethyl radicals, superoxide anion, and hydroxyl radicals, contributing to oxidative stress [40].…”

Section: Discussionmentioning

confidence: 99%

High Fischer Ratio Oligopeptides of Gluten Alleviate Alcohol-Induced Liver Damage by Regulating Lipid Metabolism and Oxidative Stress in Rats

Zhao,

Hou,

Chen

et al. 2024

Foods

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High Fischer ratio oligopeptides (HFOs) exhibit diverse biological activities, including anti-inflammatory and antioxidant properties. HFOs from gluten origin were prepared through fermentation and enzymatic hydrolysis and then characterized using free amino acid analysis and scanning electron microscopy (SEM). Following intervention, the levels of serum total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and hepatic malondialdehyde (MDA) in the rats significantly decreased (p < 0.05). Simultaneously, there was an increasing trend in superoxide dismutase (SOD) levels, and glutathione (GSH) levels were significantly elevated (p < 0.05). The mRNA expression levels of alcohol metabolism-related genes (ADH4, ALDH2, and CYP2E1) exhibited a significant increase (p < 0.05). Histological examination revealed a reduction in liver damage. The findings indicate that high Fischer ratio oligopeptides, prepared through enzymatic and fermentation methods, significantly improve lipid levels, ameliorate lipid metabolism disorders, and mitigate oxidative stress, and exhibit a discernible alleviating effect on alcoholic liver injury in rats.

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Section: Discussionmentioning

confidence: 99%

High Fischer Ratio Oligopeptides of Gluten Alleviate Alcohol-Induced Liver Damage by Regulating Lipid Metabolism and Oxidative Stress in Rats

Zhao,

Hou,

Chen

et al. 2024

Foods

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“…8,9 Bacillus subtilis and Lactococcus lactis coexpressing alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase have been selected to alleviate acute alcoholic liver injury in mice. 10,11 The engineered bacteria, while useful, rely on the expression system and are vulnerable to the GI environment. Therefore, there is high demand for an advanced delivery system to exert the potential of bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…Until now, food, herbal medicines, and enzymes were selected as chasers to alleviate adverse effects after alcohol withdrawal. − However, the above administrations cannot reduce alcohol absorption at all in the gastrointestinal (GI) tract before entering the blood, and the dealcoholization effect is extremely limited. Bioconversion is an ideal strategy for utilizing alcohol directly in the GI tract. , Bacillus subtilis and Lactococcus lactis co-expressing alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase have been selected to alleviate acute alcoholic liver injury in mice. , The engineered bacteria, while useful, rely on the expression system and are vulnerable to the GI environment. Therefore, there is high demand for an advanced delivery system to exert the potential of bacteria.…”

Section: Introductionmentioning

confidence: 99%

Bacteria-Loaded Gastro-Retention Oral Delivery System for Alcohol Abuse

Cheng

Xie

et al. 2023

ACS Biomater. Sci. Eng.

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Alcohol abuse is harmful to human health, and many strategies have been developed to retard this harm through protecting liver or activating relative enzymes. In this study, a new strategy of decreasing the alcohol absorption directly depending on the dealcoholization by the bacteria in the upper gastrointestinal (GI) tract was reported. To realize this, a bacteria-loaded gastroretention oral delivery system with pore structure was constructed through emulsification/internal gelation, which could relieve acute alcohol intoxication in mice successfully. It was found that this bacteria-loaded system kept the above 30% suspension ratio in the simulated gastric fluid for 4 min, displayed good protection effect for the bacteria, and decreased the alcohol concentration from 50 to 30% below within 24 h in vitro. The in vivo imaging results demonstrated that it remained in the upper GI tract until 24 h and reduced 41.9% alcohol absorption. The mice with oral administration of the bacteria-loaded system were found with normal gait, smooth coat, and less liver damage. Although the intestinal flora distribution was influenced slightly during the oral administration, it could restore to normal levels only one day after stopping oral administration quickly, suggesting good biosafety. In conclusion, these results revealed that the bacteria-loaded gastro-retention oral delivery system might intake alcohol molecules rapidly and has huge potential in the treatment of alcohol abuse.

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An orally delivered bacteria‐based coacervate antidote for alcohol detoxification

Qiao

Zeng

et al. 2023

Biomaterials

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Alleviating acute alcoholic liver injury in mice with Bacillus subtilis co-expressing alcohol dehydrogenase and acetaldehyde dehydrogenase

Cited by 25 publications

References 42 publications

High Fischer Ratio Oligopeptides of Gluten Alleviate Alcohol-Induced Liver Damage by Regulating Lipid Metabolism and Oxidative Stress in Rats

High Fischer Ratio Oligopeptides of Gluten Alleviate Alcohol-Induced Liver Damage by Regulating Lipid Metabolism and Oxidative Stress in Rats

Bacteria-Loaded Gastro-Retention Oral Delivery System for Alcohol Abuse

An orally delivered bacteria‐based coacervate antidote for alcohol detoxification

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