Allergy

Pepys, J.

doi:10.1007/978-94-011-6191-6_7

Cited by 4 publications

(2 citation statements)

References 115 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[1][2][3] Due to the large size of the aggregates and their intrinsic heterogeneity, studies of protein aggregates present a great challenge to the structural biologist. While X-ray crystallography struggles with the poor solubility and complexity of amyloid assemblies, and cryo-electron microscopy fails to provide images at atomic resolution, NMR spectroscopy remains the method of choice in the investigation of amyloidoses.…”

mentioning

confidence: 99%

Characterization of Amyloid Fibrils of Human β‐2‐Microglobulin by High‐Resolution Magic‐Angle Spinning NMR

2010

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

Characterization of Amyloid Fibrils of Human β‐2‐Microglobulin by High‐Resolution Magic‐Angle Spinning NMR

2010

View full text Add to dashboard Cite

show abstract

“…In vivo, SAP is a normal circulating plasma protein but it is also found associated with the extracellular matrix [1,2] and all forms of amyloid deposits, including those associated with Alzheimer's disease [3][4][5]. Therefore, it presents possibilities for both liquid phase and solid phase interactions with potential ligands.…”

Section: Introductionmentioning

confidence: 99%

Binding of Complement Proteins C1q and C4bp to Serum Amyloid P Component (SAP) in Solid Contra Liquid Phase

et al. 1996

View full text Add to dashboard Cite

Serum amyloid P component (SAP), a member of the conserved pentraxin family of plasma proteins, binds calcium dependently to its ligands. The authors investigated SAPs interaction with the complement proteins C4b binding protein (C4bp) and C1q by ELISA, immunoelectrophoresis and electron microscopy. Binding of these proteins to SAP was demonstrated when SAP was immobilized using F(ab')2 anti-SAP, but not when SAP reacted with these proteins in liquid phase; thus the binding to human SAP was markedly phase state dependent. Presaturation of solid phase SAP with heparin, which binds SAP with high affinity, did not interfere with the subsequent binding of C4bp or C1q to SAP. In contrast, collagen I and IV showed partial competition with the binding of C1q to SAP. Using fresh serum, immobilized native SAP bound C4bp whereas binding of C1q/C1 could not be demonstrated. Altogether the results indicate that firm binding of C1q and C4bp to SAP requires that SAP is presented on a solid phase, that C1q and C4bp react with sites distinct from the heparin binding site, and that C1q and collagen I share binding sites on SAP. Immobilized native SAP, aggregated SAP and SAP-heparansulphate complexes induced no detectable complement activation.

show abstract

IgE Antibodies to Bacteria in Patients with Bronchial Asthma

Pauwels¹,

Verschraegen²,

Straeten³

1980

Allergy

View full text Add to dashboard Cite

Bacterial respiratory infections, especially with Haemophilus influenzae and Streptococcus pneumoniae, are frequently associated with an increase of airways obstruction in patients with bronchial asthma. The possible involvement of immediate hypersensitivity in this phenomenon was studied. IgE antibodies to Haemophilus influenzae (HI) and Streptococcus pneumoniae (SPn) were investigated in the serum of 190 adult patients with bronchial asthma. The IgE antibodies were measured using a solid phase radioimmunoassay method. Living bacteria were used as solid phase. A correction of the non-specific binding of IgE was necessary. IgE antibodies to one or both bacteria were present in 55 of the 190 patients (29%). Eighteen patients were sensitive to HI, 33 to SPn and four to both bacteria. Significantly more IgE antibodies to bacteria were found in patients with demonstrable IgE antibodies to various inhalant allergens. However, the IgE antibodies to one or both bacteria were also present in 22% of patients with no other demonstrable IgE mediated hypersensitivity. The total serum IgE level in patients with IgE antibodies to bacteria was not significantly higher than in patients without hypersensitivity to these bacteria. From these data we concluded that immediate hypersensitivity to bacteria may play a role in the infectious exacerbations of bronchial asthma.

show abstract

Allergy

Cited by 4 publications

References 115 publications

Characterization of Amyloid Fibrils of Human β‐2‐Microglobulin by High‐Resolution Magic‐Angle Spinning NMR

Characterization of Amyloid Fibrils of Human β‐2‐Microglobulin by High‐Resolution Magic‐Angle Spinning NMR

Binding of Complement Proteins C1q and C4bp to Serum Amyloid P Component (SAP) in Solid Contra Liquid Phase

IgE Antibodies to Bacteria in Patients with Bronchial Asthma

Contact Info

Product

Resources

About