Allergic risk assessment of genetically modified cucumber mosaic virus resistant pepper

Abstract

Genetically modified (GM) pepper H15 containing the gene for cucumber mosaic virus (CMV) coat protein (CP) and its control line non-GM pepper P2377 were investigated for their allergic risk. Amino acid sequence of the inserted gene product CMV-CP was compared with those of known allergens. No known allergen had greater than 35% amino acid sequence homology over an 80 amino acid window or more than 8 consecutive identical amino acids. Protein patterns of GM and non-GM pepper extracts were evaluated by SDS-PAGE, which showed similar distribution of protein bands for both GM and non-GM pepper. Antigen-antibody reactions were compared between GM and its non-transgenic parental control. ELISA and immunoblot analysis of sera from allergic patients showed some IgE reactivity; however, no differences were observed between GM pepper H15 and P2377. We therefore conclude that CMV-CP is less likely to be an allergen; the protein composition and allergenicity of the GM pepper H15 is not different from that of P2377 and safe as a commercial host.

Changes in Germination Rate and Growth of CMV-Resistant GM Pepper and Non-GM Peppers (Capsicum annuum L.) as Influenced by Overwintering Condition and Fruit Drying Method

“…본 시험에서 일반 고추와 CMV 내성 GM 고추의 월동시험에서 발아 및 입모율 등의 차이가 없었는데, 이는 'H-15', 'P-2377'을 이용하여 실시한 농업적 특성 평가 (Cho et al, 2009), 알레르기 시험 (Son, 2015)…”

“…개발된 GM 작물을 재배용으로 이용하기 위해서는 안전성 평가를 통해 위해성 심사를 통과한 후 품종등록이 되어야 한다. 따라서 본 연구는 형질전환작물의 안전성평가와 품종등록 (Lee et al, 2008;RDA, 2009) 조건에 맞추기 위하여 바이러스 내성 GM 고추의 환경 적응성 시험 (Cho et al, 2009;Ryu et al, 2009;Son, 2015) (Gao, 2010).…”