Allergenome characterization of the mosquito Aedes aegypti

Cantillo, José Fernando; Puerta, Leonardo; Puchalska, Patrycja; Lafosse-Marin, Sylvie; Subiza, José Luis; Fernández-Caldas, Enrique

doi:10.1111/all.13150

Cited by 31 publications

(31 citation statements)

References 44 publications

(57 reference statements)

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“…This supports the shift to recombinant salivary components for the study of cat flea hypersensitivity. In any case, proteomic characterization of cat flea extract yielded interesting information for other respiratory allergic diseases that could by induced by insect inhalation [30-33]. The C. felis extract was found to be rich in muscle-skeletal proteins such as tropomyosin, ankirins, and troponins, which are usually highly conserved in invertebrates and have been reported as allergenic in several sources of epidemiological importance such as house dust mites, cockroaches, and mosquitoes.…”

Section: Discussionmentioning

confidence: 99%

Cellular and Humoral Responses to Cte f 2, a Cat Flea Allergen, in Children with Papular Urticaria

Sabogal

Lozano

Mercado

et al. 2019

Int Arch Allergy Immunol

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Background: Papular urticaria (PU) is a common insect bite skin hypersensitivity in tropical countries. In order to gain insight into its causal allergens, we aimed to evaluate cellular and humoral immune responses to the recombinant salivary antigen Cte f 2 from the cat flea Ctenocephalides felis. Method: Sixty patients with PU and 27 healthy controls were included in this study. Specific IgE, IgG, IgG1, and IgG4 against Cte f 2 and C. felis extract were determined by ELISA. The T-cell response was analyzed using a carboxyfluorescein succinimidyl ester (CFSE)-based dilution assay and Th1/Th2/Th17 cytokine measurements. In addition, a proteomic analysis of IgG and IgE reactive spots of C. felis extract was performed. Results: The frequency of IgE sensitization to Cte f 2 was similar between patients (36.7%) and controls (40.7%). The specific IgE, IgG1, and IgG4 responses to Cte f 2 and C. felis extract were not significantly different between patients and controls. Among the 3 conditions (i.e., Cte f 2, C. felis extract, and only medium) Cte f 2 was the strongest inducer of CD3+CD4+ proliferation in the patients; however, the mean response was not significantly different from those in controls (Cte f 2: 4.5 vs. 2.5%; p = 0.46). No salivary proteins were identified in C. felis, and most of the spots were identified as muscle-skeletal components (tropomyosin, actin, myosin, and ankirin). Conclusions: Cte f 2 induces IgE and IgG production as well as T-cell proliferation in children living in a geographical area where PU induced by a flea bite is common. The use of C. felis extract is not recommended for the study of bite-induced hypersensitivity disease since salivary antigens are not well represented.

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Section: Discussionmentioning

confidence: 99%

Cellular and Humoral Responses to Cte f 2, a Cat Flea Allergen, in Children with Papular Urticaria

Sabogal

Lozano

Mercado

et al. 2019

Int Arch Allergy Immunol

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“…6 HDM and mosquitoes are evolutionarily related as part of the Phylum Arthropoda. 8,9 We previously demonstrated that natural and recombinant A. aegypti tropomyosins cross-react with tropomyosin from HDM. 8,9 We previously demonstrated that natural and recombinant A. aegypti tropomyosins cross-react with tropomyosin from HDM.…”

mentioning

confidence: 99%

“…6 HDM and mosquitoes are evolutionarily related as part of the Phylum Arthropoda. The mosquito species Aedes aegypti is also a source of several allergens [6][7][8][9] and contains Aed a 10 (tropomyosin). 8,9 We previously demonstrated that natural and recombinant A. aegypti tropomyosins cross-react with tropomyosin from HDM.…”

mentioning

confidence: 99%

Tropomyosins in mosquito and house dust mite cross‐react at the humoral and cellular level

Cantillo

Puerta

Fernández-Caldas

et al. 2018

Clin Experimental Allergy

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“…Allergic reactions to mosquito bite are underestimated due to the current lack of reliable diagnostic tools. Both “species-specific” and cross-reactive allergenic molecules have recently been described mainly in the saliva of yellow fever mosquito ( Aedes aegypti ), Asian tiger mosquito ( Aedes albopictus ), Aedes vexans , and Culex quinquefasciatus [2-4]. Few studies have been conducted on Aedes communis (Ac) [5, 6], and no Ac molecule has been registered to date in the WHO/IUIS database [7].…”

mentioning

confidence: 99%

“…1), suggesting the lack of some relevant allergenic molecules in extracts for in vitro diagnosis [2, 9]. IgE specific for Aedes and Tabanus were also significantly associated (OR: 9 [CI: 1.3–61.1], p < 0.02), as well as MUXF3 reactivity (OR: 2.9 [CI: 1.1–8.5], p = 0.03), suggestive of the presence of cross-reactive glycans (CCD) in mosquito extracts [10].…”

mentioning

confidence: 99%

Aedes communis Reactivity Is Associated with Bee Venom Hypersensitivity: An in vitro and in vivo Study

Scala

Pirrotta

Uasuf

et al. 2018

Int Arch Allergy Immunol

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Mosquito bite is usually followed by a local reaction, but severe or systemic reaction may, in rare cases, occur. Allergic reactions to Aedes communis (Ac) may be underestimated due to the lack of reliable diagnostic tools. In this multicenter study, 205 individuals reporting large local reactions to Ac were enrolled and studied for cutaneous or IgE reactivity to Ac, Blattella germanica, Penaeus monodon, and Dermatophagoides pteronyssinus. Extract and molecular IgE reactivity to bees, wasps, hornets, and yellow jacket venoms were also studied in 119 patients with a clinical history of adverse reaction to Hymenoptera. Immunoblot (IB) analysis and immunoCAP IgE inhibition experiments were carried out in selected sera. Ac sensitization was recorded in 96 (46.8%) patients on SPT. Strict relationship between Ac and D. pteronyssinus, B. germanica, P. monodon, or Apis mellifera reactivity on SPT was observed. Ac IgE recognition was seen in 60/131 (45.8%) patients, 49 (81.6%) of them SPT positive, and 5/14 IB reactors. Ac IgE sensitization was associated with Tabanus spp, A. mellifera, Vespula vulgaris, and Polistes dominula reactivity. A strict relationship between Ac IgE reactivity and Api m 1, Api m 2, Api m 3, Api m 5, and Api m 10 was recorded. IgE reactivity to AC was inhibited in 9/15 cases after serum absorption with the A. mellifera extract. Both SPT and IgE Ac reactivity is observed in about half of patients with a history of large local reactions to mosquito bites. The significant relationship between Ac sensitization and either extract or single bee venom components is suggestive of a “bee-mosquito syndrome” occurrence.

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Allergenome characterization of the mosquito Aedes aegypti

Abstract: The newly identified allergens may play a role in the pathophysiology of mosquito allergy in the tropics, and some of them might be important arthropod-related proteins involved in cross-reactivity between A. aegypti and other allergenic arthropods.

Cited by 31 publications

References 44 publications

Cellular and Humoral Responses to Cte f 2, a Cat Flea Allergen, in Children with Papular Urticaria

Cellular and Humoral Responses to Cte f 2, a Cat Flea Allergen, in Children with Papular Urticaria

Tropomyosins in mosquito and house dust mite cross‐react at the humoral and cellular level

<b><i>Aedes communis</i></b> Reactivity Is Associated with Bee Venom Hypersensitivity: An in vitro and in vivo Study

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