1989
DOI: 10.1159/000234973
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Allergenic Fragments of Ryegrass <i>(Lolium perenne)</i> Pollen Allergen <i>Lol </i><i>p</i> IV

Abstract: To facilitate studies on establishing the nature of structure/function relationships of allergens, ryegrass pollen allergen, Lol p IV, was cleaved into smaller fragments by cyanogen bromide (CNBr) and the resulting peptides were further digested with trypsin. The resulting peptides were then fractionated by high performance liquid chromatography (HPLC) on a C-18 reverse phase column. The allergenic activity of the HPLC fractions was evaluated in terms of their ability to inhibit the binding of 125l-… Show more

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Cited by 11 publications
(4 citation statements)
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“…Most monoclonal antibodies to pollen allergens reported to date have a degree of cross-reactivity, some react to other grasses [1][2][3][4][5], and others even react to non-grasses [6][7][8][9][10][11][12].…”
Section: Introductionmentioning
confidence: 99%
“…Most monoclonal antibodies to pollen allergens reported to date have a degree of cross-reactivity, some react to other grasses [1][2][3][4][5], and others even react to non-grasses [6][7][8][9][10][11][12].…”
Section: Introductionmentioning
confidence: 99%
“…In addition, other reagents such as cynogen bromide and 2-nitro-5-thiocyanobcnzoic acid had also been applied for the studies of B cell epitopes with the use o f polyclonal antibodies [19,20], Peptide chains are cleaved by cyanogen bromide at the methionine, and cleaved by 2-nitro-5-thiocyanobenzoic acid at the cysteine. In the amino acid composition of Cyn d 1, there are 3-5 methionine residues, 7 cysteine residues, and 24-31 lysine residues [6,7].…”
Section: Discussionmentioning
confidence: 99%
“…with MoAb (218,221] withMoAb [211) with MoAb [237,238] with MoAb, IgE [243] T-cell epitopes yes [228,229] yes [2341 yes [234] Reombinant production yes 1195] yes [202] yes [209,212] yes [219] yes [230] yes [230,232] yes [240] trypsin inhibito (BMAI-1) [259] a-amylase / fypsin ir>hibitor (261] profilin [264] pectase lyase (266] polyalactunsnase [270]…”
Section: ]mentioning
confidence: 99%