Allergenic food extracts. I. Methods of preparation

Studies of Hypersensitivity to Fish

“…Many other foods and inhalants probably have a similarly complex antigenic composition (7,25,32). The study shows the need for better standardization of allergen extracts, as has been pointed out several times in the allergologic literature (6,14,21,33,34,35,36). If standardized extracts are not used neither skin test reactions, passive transfer reactions nor in vitro studies can be considered suitable for comparing.…”

Food allergen extracts to diagnose food-induced allergic diseases

“…Although the scale and available technologies for extract preparation have changed markedly since the process was first described, many of the manufacturing steps remain unchanged. 33,34 In general, the following procedures apply to the preparation of food extracts: grinding, defatting, extraction, clarification, sterilization, and product testing (Table 2). 23,33 After careful selection and preservation of the allergen source material, foods may undergo preliminary processing, such as grinding or blending, which increases the surface area of the material before extraction.…”

Studies of Hypersensitivity to Fish

“…Allergenic extracts have been prepared by several different me thods (31,32).Individual factors that may vary from one laboratory to another include degree of subdivision of the material, the use or not of defattening procedures, composition and ionic strength as well as pH of the extracting fluids, technique of filtration, dialysis, clarification and sterilization. When composite protein material is extracted, the factors mentioned influence the cpiality of the final extract (6,16,31,32).…”

“…When composite protein material is extracted, the factors mentioned influence the cpiality of the final extract (6,16,31,32). It has not been attempted in the present study to evaluate these factors critically.…”

“…Even when the groups of patients and the testing techniques seem commensurable, there may be divergences in the results obtained caused by differences in materials used for testing (30,31). The value of many investigations in this field is limited by the use of ill-defined allergen extracts.…”