Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice

Garbani, Mattia; Xia, Wei; Rhyner, Claudio; Prati, Moira; Scheynius, Annika; Malissen, Bernard; Engqvist, Håkan; Maurer, Marcus; Crameri, Reto; Terhorst‐Molawi, Dorothea

doi:10.1111/all.13041

Cited by 14 publications

(8 citation statements)

References 20 publications

(24 reference statements)

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“…A plethora of animal models for specific allergen immunotherapy tried optimizing the application mode and route of allergen, by entrapment in tolerogenic PLG particles [46 ▪▪ ], or application concomitantly with CpG [45], probiotics or Vitamin D3 (Table 1) [37–39,41,42 ▪ ,43 ▪▪ ,44,45,46 ▪▪ ,49 ▪ ,51]. In particular, epicutaneous application has achieved promising results without adjuvants [43 ▪▪ ].…”

Section: Resultsmentioning

confidence: 99%

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Outstanding animal studies in allergy II. From atopic barrier and microbiome to allergen-specific immunotherapy

Jensen‐Jarolim

Pali‐Schöll

Roth‐Walter

2017

Current Opinion in Allergy &Amp; Clinical Immunology

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Purpose of reviewAnimal studies published within the past 18 months were assessed, focusing on innate and specific immunomodulation, providing knowledge of high translational relevance for human atopic and allergic diseases.Recent findingsAllergic companion animals represent alternative models, but most studies were done in mice. Atopic dermatitis mouse models were refined by the utilization of cytokines like IL-23 and relevant skin allergens or enzymes. A novel IL-6 reporter mouse allows biomonitoring of inflammation. Both skin pH and the (transferable) microflora have a pivotal role in modulating the skin barrier. The microflora of the gastrointestinal mucosa maintains tolerance to dietary compounds and can be disturbed by antiacid drugs. A key mouse study evidenced that dust from Amish households, but not from Hutterites protected mice against asthma. In studies on subcutaneous and sublingual allergen-specific immunotherapy, much focus was given on delivery and adjuvants, using poly-lacto-co-glycolic particles, CpGs, probiotics or Vitamin D3. The epicutaneous and intralymphatic routes showed promising results in mice and horses in terms of prophylactic and therapeutic allergy treatment.SummaryIn atopic dermatitis, food allergies and asthma, environmental factors, together with the resident microflora and barrier status, decide on sensitization versus tolerance. Also allergen-specific immunotherapy operates with immunomodulatory principles.

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Section: Resultsmentioning

confidence: 99%

“…Strontium-doped hydroxyapatite porous spheres have been used in subcutaneous immunotherapy trials in mice to reduce side-effects, resulting in reduced symptoms after nasal challenge despite the higher percentage of eosinophils found in the bronchial lavage samples [41]. …”

Section: Immunomodulation By Allergen-specific Immunotherapymentioning

confidence: 99%

Outstanding animal studies in allergy II. From atopic barrier and microbiome to allergen-specific immunotherapy

Jensen‐Jarolim

Pali‐Schöll

Roth‐Walter

2017

Current Opinion in Allergy &Amp; Clinical Immunology

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“…Hydroxyapatite has also been investigated as a novel adjuvant and provided greater increases in IgE when bound with ovalbumin compared with soluble ovalbumin, but other exciting prospects come from work to develop advanced antibody vaccines for infectious diseases and cancer, which has stimulated the development of new adjuvants or delivery systems. Some of these may be relevant for AIT.…”

Section: Advances In Allergen Immunotherapymentioning

confidence: 99%

“…Similarly, these nanoparticles have been shown to enhance IL10, IgG1 and CD8 responses, and increase Th2 immunity, whereas allergists would be looking for enhanced IgG4 and reduced Th2 immunity. Although liposomes and hydrogel formulation of drugs for inflammatory skin disease have been approved for use, to date, uses in AIT have only been investigated in mice . This means there are no safety data for this nanoparticle plus allergen combination.…”

Section: Advances In Allergen Immunotherapymentioning

confidence: 99%

Perspectives in allergen immunotherapy: 2017 and beyond

Pfaar

Бонини

Cardona

et al. 2018

Allergy

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The Future of the Allergists and Specific Immunotherapy (FASIT) workshop provides a regular platform for global experts from academia, allergy clinics, regulatory authorities and industry to review developments in the field of allergen immunotherapy (AIT). The most recent meeting, held in February 2017, had two main themes: advances in AIT and hot topics in AIT from the regulatory point of view. The first theme covered opportunities for personalized AIT, advances in adjuvants and delivery systems, and the development of new molecules and future vaccines for AIT. Key topics in the second part of the meeting were the effects of the enactment of European Directive 2001/83 on the availability of allergens for therapy and diagnosis across the EU, the challenges of conducting Phase 3 studies in the field, the future role of allergen exposure chambers in AIT studies and specific considerations in performing AIT studies in the paediatric population. Finally, the group highlighted the forthcoming EAACI guidelines and their particular importance for the standardization of practice in the treatment of allergies. This review presents a comprehensive insight into those panel discussions and highlights unmet needs and also possible solutions to them for the future.

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“…Many studies are currently dedicated to maintain the appropriate balance between mechanical strength and controlled degradation ability of CPC. In this context, Sr 2+ , Zn 2+ and Cu 2+ substitutions in CPC have been found to alter the resorption rate; however, with limited success to achieve the desired mechanical features (Bandyopadhyay, Bernard, Xue, & Bose, ; Garbani et al, ; Knabe et al, ; Šupová, ). Moreover, the attempt to incorporate Ti 4+ , Al 3+ and Zr 4+ in CPC also fails to provide appropriate mechanical strength, which is mainly contributed by the limited occupancy of these ions in either HAP or β ‐TCP lattice alongside their ability to crystallize as secondary phase in the form of individual oxides namely TiO 2 , Al 2 O 3 and ZrO 2 (Goldberg et al, ; Kumar & Kannan, ; Seeley, Bandyopadhyay, & Bose, ; Vladescu et al, ).…”

Section: Introductionmentioning

confidence: 99%

Investigation on the structural, mechanical and in vitro biocompatibility features of CaZr₄(PO₄)₆ influenced by Zn²⁺ substitutions

et al. 2019

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The present study explores the possibility of Zn2+ substituted calcium zirconium phosphate [CaZr4(PO4)6] as a potential replacement for the existing materials in load bearing orthopedic applications. Pure CaZr4(PO4)6) and wide range of Zn2+ substitutions in CaZr4(PO4)6 have been synthesized through citrate assisted sol–gel technique. The characterization results confirmed the extraordinary structural stability displayed by CaZr4(PO4)6 until 1,550°C. Further, the flexibility of CaZr4(PO4)6 lattice to accommodate 40 mol% of Zn2+ has been determined. The microstructures of CaZr4(PO4)6 and Zn2+ substituted CaZr4(PO4)6 demonstrated irregular sized grains and cracks alongside the negligence to obtain definite grain boundaries. This has been reflected in the moderate mechanical properties of the investigated specimen; nevertheless, Zn2+ substituted CaZr4(PO4)6 displayed enhanced mechanical stability. Further, in vitro tests signified the remarkable biocompatibility and alkaline phosphatase activity of Zn2+ substituted CaZr4(PO4)6.

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Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice

Abstract: We conclude that SHAS may constitute a suitable carrier and adjuvant for ASIT with great potential due to its unique protein-binding properties.

Cited by 14 publications

References 20 publications

Outstanding animal studies in allergy II. From atopic barrier and microbiome to allergen-specific immunotherapy

Outstanding animal studies in allergy II. From atopic barrier and microbiome to allergen-specific immunotherapy

Perspectives in allergen immunotherapy: 2017 and beyond

Investigation on the structural, mechanical and in vitro biocompatibility features of CaZr₄(PO₄)₆ influenced by Zn²⁺ substitutions

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Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice

Abstract: We conclude that SHAS may constitute a suitable carrier and adjuvant for ASIT with great potential due to its unique protein-binding properties.

Cited by 14 publications

References 20 publications

Outstanding animal studies in allergy II. From atopic barrier and microbiome to allergen-specific immunotherapy

Outstanding animal studies in allergy II. From atopic barrier and microbiome to allergen-specific immunotherapy

Perspectives in allergen immunotherapy: 2017 and beyond

Investigation on the structural, mechanical and in vitro biocompatibility features of CaZr4(PO4)6 influenced by Zn2+ substitutions

Contact Info

Product

Resources

About

Investigation on the structural, mechanical and in vitro biocompatibility features of CaZr₄(PO₄)₆ influenced by Zn²⁺ substitutions