Allelopathic potential of root endophytic fungal metabolites of Casuarina equisetifolia

Huang, Rui; Jin, Shaowu; Wang, X.; Xu, Zhao-Long; Li, H. M.; Li, L.

doi:10.26651/allelo.j./2018-45-2-1188

Cited by 5 publications

(6 citation statements)

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“…Lin et al [17] isolated 65 strains of different endophytic fungi from the roots, stems, and leaves of C. equisetifolia. In addition, five strains of fungi and four strains of endophytic fungi had been isolated from rhizosphere soil and root of C. equisetifolia, respectively [18,19]. These previous studies are limited to the isolation and identification of culturable endophytes and the determination of their biological activities, so they may not be sufficiently accurate and/or are able to analyze only a limited set of microbial species in samples from complex environments and, thus, do not allow a comprehensive understanding of the effects of planting years on rhizospheric and endophytic fungi communities.…”

Section: Introductionmentioning

confidence: 99%

Structural variability and niche differentiation of the rhizosphere and endosphere fungal microbiome of Casuarina equisetifolia at different ages

et al. 2020

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Background Casuarina equisetifolia is one of the most important artificially planted protective forests along the coast in southern China for windbreaks, soil erosion, and sand dune stabilization. Self-renewing of C. equisetifolia is very limited, which might be caused by low soil nutrient levels and reduced microbial activity. Methods Use of high-throughput sequencing of the 18S rDNA to investigate the microbial communities from the rhizosphere and root endosphere of C. equisetifolia in young-aged, intermediate-aged, and mature-aged forests. Results Our results indicate that the diversity of rhizosphere fungal microbiomes in field-grown C. equisetifolia is much lower than that of the endosphere microbiomes. Bioinformatic analysis showed that rhizocompartments produce the strongest differentiation of rhizosphere and endosphere communities. Notably, the distribution of rhizosphere fungi communities was significantly influenced by the environmental factors, not by forest ages. Conclusions The presented study suggests that the rhizocompartments and environmental factors, rather than forest ages, determine the diversities of fungal community.

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Section: Introductionmentioning

confidence: 99%

Structural variability and niche differentiation of the rhizosphere and endosphere fungal microbiome of Casuarina equisetifolia at different ages

et al. 2020

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“…Meanwhile, low concentrations of 2,4-DTBP (60 mM) can aggregate rhizosphere soil bacteria, e.g., Bacillus cereus strain CP1, Pseudomonas sp. and Enterobacter hormaechei strain AMS-38 (Huang et al, 2018;Lin et al, 2022). Cui et al (2022) also found that microbial biomass was stimulated with the addition of 0.5 mM 2,4-DTBP and inhibited with the addition of 2.0 mM 2,4-DTBP.…”

Section: Discussionmentioning

confidence: 90%

Is allelochemical synthesis in Casuarina equisetifolia plantation related to litter microorganisms?

Zuo

Zhang

et al. 2022

Front. Plant Sci.

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Productivity decline of Casuarina equisetifolia plantation and difficulty in natural regeneration remains a serious problem because of allelopathy. Previous studies have confirmed that 2,4-di-tert-butylphenol (2,4-DTBP) are the major allelochemicals of the C. equisetifolia litter exudates. The production of these allelochemicals may derive from decomposition of litter or from the litter endophyte and microorganisms adhering to litter surfaces. In the present study, we aimed to evaluate the correlation between allelochemicals in litter and endophytic and epiphytic fungi and bacteria from litter. A total of 100 fungi and 116 bacteria were isolated from the interior and surface of litter of different forest ages (young, half-mature, and mature plantation). Results showed that the fermentation broth of fungal genera Mycosphaerella sp. and Pestalotiopsis sp., and bacterial genera Bacillus amyloliquefaciens, Burkholderia-Paraburkholderia, and Pantoea ananatis had the strongest allelopathic effect on C. equisetifolia seeds. Allelochemicals, such as 2,4-DTBP and its analogs were identified in the fermentation broths of these microorganisms using GC/MS analysis. These results indicate that endophytic and epiphytic fungi and bacteria in litters are involved in the synthesis of allelochemicals of C. equisetifolia. To further determine the abundance of the allelopathic fungi and bacteria, Illumina MiSeq high-throughput sequencing was performed. The results showed that bacterial genera with strong allelopathic potential were mainly distributed in the young and half-mature plantation with low abundance, while the abundance of fungal genera Mycosphaerella sp. and Pestalotiopsis sp. were higher in the young and mature plantations. In particular, the abundance of Mycosphaerella sp. in the young and mature plantations were 501.20% and 192.63% higher than in the half-mature plantation, respectively. Overall, our study demonstrates that the litter fungi with higher abundance in the young and mature plantation were involved in the synthesis of the allelochemical 2,4-DTBP of C. equisetifolia. This finding may be important for understanding the relationship between autotoxicity and microorganism and clarifying the natural regeneration problem of C. equisetifolia.

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“…We refer to previous methods for analyzing the soil chemistry of leaves, roots and soils ( Huang, 2019 ). The organic carbon, total nitrogen, total phosphorus, and total potassium contents were determined by chromic acid oxidation, Kjeldahl determination of nitrogen, Mo–Sb colorimetric method, and flame photometry, respectively.…”

Section: Methodsmentioning

confidence: 99%

Ecological niche selection shapes the assembly and diversity of microbial communities in Casuarina equisetifolia L.

Lin

Wang

et al. 2022

Front. Plant Sci.

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The plant microbiome profoundly affects many aspects of host performance; however, the ecological processes by which plant hosts govern microbiome assembly, function, and dispersal remain largely unknown. Here, we investigated the bacterial and fungal communities in multiple compartment niches (bulk soil, rhizosphere soil, root endosphere, phylloplane, and leaf endosphere) of Casuarina equisetifolia L. at three developmental stages in Hainan Province, China. We found that microbiome assemblages along the soil–plant continuum were shaped by the compartment niches. Bacterial diversity and richness decreased from the soils to roots to leaves, with the highest network complexity found in the roots and the lowest found in the phylloplane. However, fungal diversity gradually increased from the soils to roots to phyllosphere, whereas fungal richness decreased from the soils to roots but increased from the roots to phyllosphere; the greatest network complexity was found in bulk soils and the lowest was found in the roots. Different biomarker taxa occurred in the different ecological niches. Bacterial and fungal communities exhibited distinct ecological functions; the former played important roles in maintaining plant growth and providing nutrients, whereas the latter predominantly decomposed organic matter. The bacterial community of C. equisetifolia mostly originated from bulk soil, whereas the fungal community was mainly derived from rhizosphere soil and air. Leaf endophytes were positively correlated with organic carbon, and root and soil microorganisms were positively correlated with total nitrogen, total phosphorus, and total potassium. Our findings provide empirical evidence for plant–microbiome interactions and contribute to future research on non-crop management and the manipulation of non-crop microbiomes.

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Allelopathic potential of root endophytic fungal metabolites of Casuarina equisetifolia

Cited by 5 publications

References 0 publications

Structural variability and niche differentiation of the rhizosphere and endosphere fungal microbiome of Casuarina equisetifolia at different ages

Structural variability and niche differentiation of the rhizosphere and endosphere fungal microbiome of Casuarina equisetifolia at different ages

Is allelochemical synthesis in Casuarina equisetifolia plantation related to litter microorganisms?

Ecological niche selection shapes the assembly and diversity of microbial communities in Casuarina equisetifolia L.

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