Allelic state and effects of VRN genes on soft wheat in in vivo and in vitro systems

Aksentyeva, O O; Shulik, Victoriia V.

doi:10.15421/011627

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…If the integrity of plant organism is likely compromised, the functional stability of VRN gene system, i. e. the need for vernalization, is kept. This statement is confi rmed by our previously fi ndings [4] and the reported data [22], according to which the winter wheat cultivars showed the change of recessive state to dominant VRN-B1 gene (the main repressor of its transition to generative development) as occurred during vernalization. In addition, we have shown that under in vitro conditions in the wheat isogenic проблеми кріобіології і кріомедицини problems of cryobiology and cryomedicine том/volume 32, №/issue 3, 2022 сліджуваних сортів порівняно з контролем ризогенез відбувався більш інтенсивно тільки на 45 добу.…”

Section: ефективність первинного калюсогенезу сортів пшениці м'якої о...supporting

confidence: 75%

“…The exposure of wheat and barley ears, potato and tobacco buds at 7-14°C was shown to augment the embryoid number [8]. The impact of low проблеми кріобіології і кріомедицини problems of cryobiology and cryomedicine том/volume 32, №/issue 3, 2022 ню кількості ембріоїдів [4]. Вплив зниженої температури на процеси морфогенезу в калюсних культурах досліджено недостатньо.…”

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Cardioprotective Effect of Low Molecular Weight (Below 5 kDa) Fraction of Cattle Cord Blood Cryohemolysate

Shcheniavsky¹,

Kovalenko²,

Gulevsky³

2022

Probl Cryobiol Cryomed

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In this report, we have studied a cardioprotective effect of low molecular weight (below 5 kDa) fraction derived from cattle cord blood in a rat model of infarction-like cardiovasopathy. The myocardial infarction development was initiated in the animals, maintained for two months on a diet with excessive intake of fat, calcium and sodium salts, by making hypoxia-hypercapnia in them. The use of this model allowed inducing the infarction-like cardiovasopathy, comparable by many respects with the clinical picture of myocardial infarction, which was confirmed by electrocardiography, enzyme-linked immunosorbent assay and histological analysis. Preliminary administration of low molecular weight (below 5 kDa) fraction of cattle cord blood, as well as Actovegin did not prevent the heart attack, but increased the endurance of rats to hypoxia-hypercapnia and reduced the damage rate to myocardium. A preventive administration of low molecular weight fraction of cord blood was proven to mitigate the risk of hypoxia-induced damages to myocardium.

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Section: ефективність первинного калюсогенезу сортів пшениці м'якої о...supporting

confidence: 75%

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Cardioprotective Effect of Low Molecular Weight (Below 5 kDa) Fraction of Cattle Cord Blood Cryohemolysate

Shcheniavsky¹,

Kovalenko²,

Gulevsky³

2022

Probl Cryobiol Cryomed

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“…It was established that winter or spring type of development of diploid wheat and barley was determined by allelic variation in the Vrn-A1 and Vrn-B1 genes, and in polyploid wheat species by allelic variation of Vrn-A1 (Fu et al, 2009). In our research, the variability of the allelic state of Vrn-B1 gene was also shown in the cultivation of primary and transplant callus culture of soft hexaploid wheat (Avksentyeva, Shulik, 2016).…”

Section: Discussionmentioning

confidence: 51%

Research into the influence of contrasting trophic conditions of vernalization on the allelic state of Vrn genes and development rates of Triticum aestivum L.

Avksentiieva¹,

Shulik²,

Taran³

2018

biologija

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The influence of contrasting trophic conditions of vernalization on the allelic state of the Vrn genes and development rates of two winter wheat varieties, Mironovskaya 808 and Olvia, was investigated. Vernalization was carried out during 45 days at the temperature 4 ± 1°С under different conditions of trophic support. Whole seeds with endosperm and isolated buds, with added water and 3% solution of sucrose, were vernalized. The allelic state of the Vrn genes was identified by PCR using allele-specific primers on the sprouts at different phases of vernalization – 15, 30, and 45 days. Phenological observations were carried out by determining of the transition to the generative development of wheat plants, cultivated from the vernalized sprouts. According to the results obtained, it was established that the recessive state of the genes Vrn-A1, Vrn-B1 and Vrn-D1 was unchanged in all variants of the two winter wheat varieties on the 15th and 30th days of vernalization. After 45 days of vernalization, the recessive and dominant alleles in sprouts, vernalized under normal trophic conditions and with added 3% solution of sucrose, were detected at the Vrn-B1. All variants of wheat plants grown from vernalized sprouts under contrasting trophic conditions were transferred to the generative phase, but at different times. It could indicate an epigenetic regulation vernalization process. It was established that different trophic conditions during vernalization affected the changes of the allelic state of the Vrn-B1 gene and determined the transition to the generative development of winter wheat.

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Morphogenetic Responses of Triticum aestivum L. Calus Culture During Long-Term Effect of Low Positive Temperatures

Avksentievа

Zhmurko

Чумакова

2022

Probl Cryobiol Cryomed

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The paper presents the findings on the impact of cold exposure at 4°C on chlorophylogenesis, hemogenesis and rhizogenesis in callus culture of winter wheat cultivars. The patterns of morphogenesis in the soft winter wheat in vitro culture during exposure to positive low temperature of various durations (15, 30 and 45 days) have been established. The callus culture of 2–3 passages of cv. Doridna, Statna and Astet was used. The results of experiments showed the preliminary vernalization of calluses for 30 and 45 days to strongly stimulate the chlorophylogenesis and hemogenesis, thereby increasing the frequency of obtaining the regenerant plants during further cultivation. The efficiency of these processes depended on the original cultivar genotype and cold treatment duration. The vernalization, associated with the VRN genes expression, manifested in morphogenesis change, was assumed to occur in the soft winter wheat in vitro culture.

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Allelic state and effects of VRN genes on soft wheat in in vivo and in vitro systems

Cited by 4 publications

References 21 publications

Cardioprotective Effect of Low Molecular Weight (Below 5 kDa) Fraction of Cattle Cord Blood Cryohemolysate

Cardioprotective Effect of Low Molecular Weight (Below 5 kDa) Fraction of Cattle Cord Blood Cryohemolysate

Research into the influence of contrasting trophic conditions of vernalization on the allelic state of Vrn genes and development rates of Triticum aestivum L.

Morphogenetic Responses of Triticum aestivum L. Calus Culture During Long-Term Effect of Low Positive Temperatures

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